Başkent Üniversitesi Yayınları
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Item Transplantation of Insulin-Producing Clusters Derived From Adult Bone Marrow Stem Cells to Treat Diabetes in Rats(Başkent Üniversitesi, 2008-09) Gabr, Mahmoud M.; Ghoneim, Mohamed A.; Refaie, Ayman F.; Zakaria, Mahmoud M.; Sobh, Mohamed M.Objectives: Recent findings suggest that bone marrow stem cells can differentiate into numerous cell types. This would provide a potentially unlimited source of isletlike cells for transplantation and a promising therapy for diabetes mellitus. Here, we studied the differentiation ability of adult bone marrow hematopoietic-rich stem cells to form glucose-regulating insulin-producing cells. Their ability to treat chemically induced diabetes in rats was then tested. Materials and Methods: Hematopoietic-rich stem cells were obtained from the long bones of rats and cultured in a serum-free medium containing 1% dimethyl sulfoxide for 3 days. The cells were cultured for 7 days in a glucose-rich medium supplemented with pancreatic extract. Thereafter, cultures were done in a medium (low concentration of glucose and 5% fetal bovine serum) supplemented with nicotinamide and exendin-4 for 7 more days. Results: At day 17 of culture, the cells formed isletlike clusters. These were distinctly stained crimson red by diphenylthiocarbazone and expressed insulin and endocrine-specific transcription genes. Insulin was secreted in a dose-response manner as a function of increasing glucose concentrations. When transplanted in the testes of diabetic rats, the differentiated cells could normalize blood glucose levels for 3 months in 80% of the treated rats. The therapeutic benefits were reversed after orchidectomy. Conclusions: Hematopoietic-rich stem cells may include pancreatic progenitor cells capable of differentiating into functioning endocrine hormone-producing cells. This finding suggests a possible means of treating diabetes mellitus.Item Tip 2 Diyabetli Yetişkin Bireylerde Diyet ile Yağ Asitleri Alımının Beslenme Durumu ile İlişkisinin Belirlenmesi(Başkent Üniversitesi, 2021-12-30) Cankar,Bilge; Saka,MendaneAmaç: Bu çalışma tip 2 diyabetli bireylerde diyet yağ asitleri alımının diyabetik parametreler ile beslenme durumu üzerindeki etkisini belirlemek amacıyla yapılmıştır. Gereç ve Yöntem: Çalışma Ocak-Mart 2020 tarihleri arasında Gaziantep Üniversitesi Şahinbey Araştırma ve Uygulama Hastanesi Endokrinoloji polikliniğine gelen, Tip 2 diyabet tanısı almış 20-65 yaş arası 48 birey üzerinde yürütülmüştür. Araştırmaya katılan Tip 2 diyabetli bireylere genel özellikleri, sağlık durumları ve beslenme alışkanlıkları, fiziksel aktivite durumları ile ilgili tanımlayıcı bilgileri içeren 38 adet çoktan seçmeli sorulardan oluşan anket formu uygulanmıştır. Bireylerin enerji, makro ve mikro besin ögeleri ile diyetle aldıkları yağ asitlerinin çeşit ve miktarını belirlemek için 3 günlük besin tüketim kaydı alınmıştır. Bulgular: Çalışmaya katılan tip 2 diyabetli bireylerin doymuş yağ asidi tüketim miktarları ile HbA1c düzeyleri arasında pozitif ilişki saptanmıştır (p=0.03). Yaş ve cinsiyeti dahil ederek yapılan model 1’de doymuş yağ asitleri tüketim miktarının HbA1c düzeyleri üzerinde anlamlı bir fark oluşturmadığı belirlenirken, yaş ve cinsiyete ek olarak bireylerin yaşadığı yer, eğitim durumu, mesleği, medeni hali, tip 2 diyabet süresi, tip 2 diyabet dışındaki kronik hastalıkları, aile diyabet öyküsü, fiziksel aktivite durumu ve BKİ değerlerinin eklenmesiyle elde edilen model 2’de doymuş yağ asidi tüketim miktarı ile HbA1c düzeyleri arasında pozitif ilişki saptanmıştır (p=0.016). Model 1’de bireylerin çoklu doymamış yağ asitleri tüketim miktarı ile HbA1c düzeyleri arasında anlamlı bir ilişki saptanmazken (p=547), model 2’de negatif ilişki tespit edilmiştir (p=0.006). Sonuç: Bu çalışma, diyetle alınan yağ miktarı ile birlikte yağ asit örüntüsünün de tip 2 diyabet yönetimini etkilediği konusunda aydınlatıcı olabilir.Item Clinical Presentation and Outcomes of Burn Patients With Diabetes: A 5-Year Single-Center Experience(Başkent Üniversitesi, 2022-09) Santiago J. Santelis; Cem Aydogan; Ayse Ebru Abali; Hasan Turkoglu; Mehmet HaberalABSTRACT OBJECTIVES: Diabetes mellitus is one of the most important independent risk factor for poor outcomes in patients with burn injuries. In this study, we aimed to determine the demographics characteristics, clinical presentation, and outcomes of burn patients with preexisting diabetes mellitus seen over the previous 5 years at a single burn center. MATERIALS AND METHODS: We conducted a retrospective study that included all burn patients ≥18 years of age with a diagnosis of diabetes who were admitted to our unit over the previous 5 years (2018 to 2022). We collected demographics and relevant clinical data from medical records. Patients were evaluated by age subgroups and time interval between occurrence of injury and admission to a medical center. RESULTS: Our study included 52 patients, with male-to-female ratio of 0.86:1 and mean age of 62.7 ± 12.4 years (range, 33-85 y). Scalding was the most common cause of the burn injury. A total of 32.7% of the patients had burns affecting their hands, either as part of a more extensive burn or as an isolated injury. The median extent of burns was 1.0% total body surface area (range, 0.05%-10%). We found an association between age and burn etiology and age and burn site. A high number of patients with injuries in the lower extremity sought medical care after day 1 but before day 4 postinjury. Delayed admission was found to be an important factor causing an increase in complication frequency. CONCLUSIONS: There are many factors associated with age that affect the incidence and outcomes of burn injuries. Risk factors leading to burns are preventable, and the physical and psychological consequences of people who survive burn injuries can be life-threatening and often devastating. The best way to treat a burn is to prevent it from happening in the first place.