Tıp Fakültesi / Faculty of Medicine
Permanent URI for this collectionhttps://hdl.handle.net/11727/1403
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Item Evaluation of the COVID-19 Rapid Antigen Test(2023) Sanli, Ozlem Oguc; Kuscu, Ozlem Ozkan; Incekas, Caner; 0000-0001-7899-0233; 0000-0001-9019-423XIntroduction: Coronavirus disease, is an infectious disease caused by the SARS-CoV-2. The gold standard method to diagnose is the reverse transcriptase polymerase chain reaction test. Rapid antigen tests can also be used for diagnosis. This study aims to compare the results of these two methods.Materials and Methods: Between November 2021 and July 2022, the study included 1811 patients who visited the emergency depart-ment with coronavirus-related symptoms and signs. Respiratory samples from these patients were simultaneously evaluated using both reverse transcriptase polymerase chain reaction and rapid antigen tests. The reverse transcriptase polymerase chain reaction tests were conducted using the BioSpeedy SARS-CoV-2 reverse transcriptase polymerase chain reaction kit (Bioeksen-Turkiye), while the rapid antigen tests were performed using the RapidForTM SARS-CoV-2 Ag (Vitrosens-Germany).Results: The comparison of the reverse transcriptase polymerase chain reaction test and rapid antigen test results showed a 90.67% sensitivity, 98.28% specificity, 91.27% positive predictive value, 98.15% negative predictive value, and 97.02% (1757/1811) accuracy. Qualitative results of both tests exhibited a very good agreement (Kappa= 0.892, p< 0.001). According to the reverse transcriptase polymerase chain reaction test, the sensitivity of the rapid antigen test was found to be 100% in 28 samples with a cycle threshold <17, 100% in 78 samples with a cycle threshold <20, 98.33% in 120 samples with a cycle threshold <22, and 96.28% in 215 samples with a cycle threshold <25.Conclusion: When the results of the study are evaluated, it is seen that the use of the rapid antigen test for screening purposes and confirmation of patients with negative test results by Reverse transcriptase polymerase chain reaction will provide advantages in terms of both time and cost. Due to the low sensitivity and high positive predictive value of the vitrosens rapid antigen test, we think that this test can be used in the first stage to accelerate the diagnosis of patients with high viral load, who are more likely to be infectious, to prevent transmission and to start their treatment quickly.Item Investigation of Group A Rotavirus G10, G12 Genotypes Emerging in Patients with Acute Gastroenteritis in a Tertiary Care Hospital(2021) Kahraman, Hande; Kocak, Aylin Altay; Albakkour, Katren; Muftah, Hager; Dalgic, Buket; Caglar, Kayhan; Ahmed, Kamruddin; Bozdayi, Gulendam; 34666655Rotaviruses are the most common cause of viral gastroenteritis with the highest mortality and morbidity rates in children aged 0-5 years. The aim of this study was to determine the frequency of rotavirus infection in patients whose stool samples were sent to microbiology laboratory to investigate the etiology of diarrhea, to investigate the rotavirus genotypes that are common in our region and G10, G12 genotypes that have recently become common in the world. Fecal samples of 476 patients aged between 0-92 years who applied between November 2016 and February 2018 were studied via immunochromatographic rapid test and enzyme-linked immunosorbent assay (ELISA) methods. ELISA positive samples were studied by nested reverse transcriptase chain reaction (RT-PCR) and genotyped by agarose gel electrophoresis. Rotavirus was found positive in 18.3% and 17% of stool samples by immunochromatographic test and ELISA, respectively. All ELISA positive samples were also detected as positive by RT-PCR. 18.5% of female patients and 15.7% of male patients were found to be positive and rotavirus positivity was not statistically significant between genders. The frequency of rotavirus in different age groups was 23.5% (6-12 years), 17.3% (13-24 months) and 16% (25-36 months). It was determined that rotavirus cases were most common in the spring. G1, G2, G3, G4, G9, G10, and G12 were detected in 37%, 7.4%, 16.1%, 6.2%, 9.9%, 2.5%, 26% of the samples, respectively. G12 was the most common genotype after G1. The most common G and P genotype combination was G1P[8] (17.2%). This was followed by G12P[8] (11.11%) and G3P[8] (11.11%). P[8] (53%) was found to be the dominant P genotype. In this study, it was observed that rotavirus, which is the cause of childhood diarrhea, can also be encountered in advanced ages and even new genotypes that infect humans worldwide may also be the causative agents. Therefore, we concluded that it is important to investigate new genotypes such as G10 and G12 in molecular epidemiological studies.Item Investigation of the Rotavirus Genotypes Isolated From Patients With Acute Gastroenteritis and the Increase of G9 Type(2021) Kocak, Aylin AltayIntroduction: Rotavirus (RV) is the most common cause of gastroenteritis in children and is one of the most common cause of mortality and morbidity in developing countries. The aim of this study was to determine the genotypes of RV rapid test antigen positive patients between 0-65 years old with acute gastroenteritis attended to a tertiary care hospital in Ankara. Materials and Methods: This study was conducted between January 2013 and April 2018 at Gazi University Faculty of Medicine, Department of Microbiology, Stool samples were collected from 87 (40 female, 47 male) patients aged between 0-65 years who had gastroenteritis were sent to microbiology laboratory. RV VP-7 amplification was performed using Beg9 and End9 primers and specific primers for G typing G1-G4 and G9. VP-4 amplification was performed using con-2 and con-3 primers. P types were determined by specific primers for P[4], P[6], P[8] and P[9]. Access Quick RT-PCR (Promega Corporation, Madison, WI) was used for VP4 and VP7 gene amplification, and PCR Mastermix (Promega, Madison, WI) was used for genotyping. Results: No statistically significant difference was found between the gender of patients with positivity of RV antigen. RV infection was most common in children aged 12-23 months. Antigen positivity was most common in winter and autumn. Genotypes G1 (25.80%), G2 (3.22%), G3 (4.30%), G4 (6.45%), G9 (60.21%) constituted G types. P4 (1.14%), P8 (93.10%), P6 (5.74%) constituted P types. The combination of G and P types was the most prevalent for G9P[8] (56.98%) and G1P[8] (22.58%). Conclusion: It has been observed that G9 and P[8] are common genotypes in cases with RV infection in Ankara as in the whole world. Similar to various studies in Turkey, genotype G9P[8] had the highest ratio in the present study. Therefore, these data should be considered in RV vaccine strategies.