Tıp Fakültesi / Faculty of Medicine
Permanent URI for this collectionhttps://hdl.handle.net/11727/1403
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Item Intraperitoneal Mesenchymal Stem Cell Administration Ameliorates Allergic Rhinitis in The Murine Model(2017) Isik, Sakine; Karaman, Meral; Adan, Aysun; Kiray, Muge; Bagriyanik, Husnu Alper; Sozmen, Sule Caglayan; Kozanoglu, Ilknur; Karaman, Ozkan; Baran, Yusuf; Uzuner, Nevin; https://orcid.org/0000-0002-5268-1210; 27380271; AAE-1241-2021Previous studies showed that bone marrow-derived mesenchymal stem cells (BMSCs) could ameliorate a variety of immune-mediated and inflammatory diseases due to their immunomodulatory and anti-inflammatory effects. In this study, we developed a mouse model of ovalbumin (OVA) induced allergic inflammation in the upper airways and evaluated the effects of the intraperitoneal administration of BMSCs on allergic inflammation. Twenty-five BALB/c mice were divided into five groups; group I (control group), group II (sensitized and challenged with OVA and treated with saline-placebo group), group III (sensitized and challenged with OVA and treated with 1 x 10(6) BMSCs), group IV (sensitized and challenged with OVA and treated with 2 x 10(6) BMSCs), and group V (sensitized and challenged with phosphate buffered saline (PBS) and treated with 1 x 10(6) BMSCs). Histopathological features (number of goblet cells, eosinophils and mast cells, basement membrane, epithelium thickness, and subepithelial smooth muscle thickness) of the upper and lower airways and BMSCs migration to nasal and lung tissue were evaluated using light and confocal microscopes. Levels of cytokines in the nasal lavage fluid and lung tissue supernatants were measured using enzyme-linked immunosorbent assay (ELISA). Confocal microscopic analysis showed that there was no significant amount of BMSCs in the nasal and lung tissues of group V. However, significant amount of BMSCs were observed in group III and IV. In OVA-induced AR groups (group II, III, and IV), histopathological findings of chronic asthma, such as elevated subepithelial smooth muscle thickness, epithelium thickness, and number of goblet and mast cells, were determined. Furthermore, the number of nasal goblet and eosinophil cells, histopathological findings of chronic asthma, and IL-4, IL-5, IL-13, and NO levels was significantly lower in both BMSCs-treated groups compared to the placebo group. Our findings indicated that histopathological findings of chronic asthma were also observed in mice upon AR induction. BMSCs migrated to the nasal and lung tissues following intraperitoneal delivery and ameliorated to the airway remodeling and airway inflammation both in the upper and lower airways via the inhibition of T helper (Th) 2 immune response in the murine model of AR.Item Which has an Influence on Mean Platelet Volume: Allergic Rhinitis or Asthma?(2022) Senol, Handan Duman; Ozdogru, Emine Ece; Sancakli, Ozlem; Dogru, Mahmut; Tuncel, TubaAim: Bronchial asthma and allergic rhinitis are mediated by similar allergic inflammatory mechanisms. Platelets play a role in allergic reactions which are inflammatory processes. The mean platelet volume (MPV) is a marker of platelet activation. This study aimed to investigate MPV value differences between children with allergic rhinitis during symptomatic or asymptomatic periods to determine whether MPV is a useful indicator of inflammation in allergic rhinitis. Materials and Methods: The records of those patients with allergic rhinitis were analyzed retrospectively. Patients over two years of age who had complete blood count results from both their asymptomatic and the symptomatic periods were included in this study. Clinical characteristics (age, age at diagnosis, symptoms, and comorbid allergic diseases) and laboratory data (thrombocyte count, MPV, white blood cell count, eosinophil count, and percentage, immunoglobulin E level, and skin prick test results) were recorded from the patient files and the hospital registry system. Results: MPV values during the symptomatic periods were statistically significantly higher than those from the asymptomatic period (p<0.001) in all patients. When the patients were grouped according to having asthma or not, MPV was found to be higher in the symptomatic period compared to the asymptomatic period in the group with asthma, but there was no difference between these two periods in the group without asthma (p=0.017, p=0.102 respectively). Additionally, MPV levels were significantly higher in the asthma group during both the symptomatic and the asymptomatic periods (p=0.04, p=0.013, respectively). Conclusion: This study suggests that MPV cannot be used as an inflammation indicator in the symptomatic period for patients with allergic rhinitis. Asthma influences MPV values. It is recommended to conduct more detailed and prospective studies to show MPV inflammation in AR.Item The Score for Allergic Rhinitis study in Turkey, 2020(2021) Eksi, ErkanObjective: This study aimed to determine how prevalent allergic rhinitis (AR) is in Turkey and to compare the current prevalence with the figures obtained 10 years earlier. Methods: This study included 9,017 participants. The minimum number of participants required from each center was determined via a stratified sampling technique according to regional demographic characteristics as ascertained from the last census. For each region, both men and women were administered the score for allergic rhinitis (SFAR) questionnaire and a score for each participant was calculated based on the responses supplied. Results: A total of 9,017 individuals (55.3% men and 44.7% women) took part in this study. Of these, 94.4% were urban residents and 5.6% lived in a rural setting. Of the men, 38.5% self-reported as suffering from AR. The corresponding figure in women was 40.5%. The overall prevalence of AR, as deduced on the basis of the SFAR, was found to be 36.7%. Comparing the prevalence in different regions, we found that AR was the least prevalent in the Black Sea region with a frequency of 35.8%. The highest prevalence was in the Mediterranean region, where the prevalence was 37.7%. There was no statistical significance in the apparent differences in prevalence between different geographical regions. Despite this, however, there was a clear increase in the frequency of AR over the preceding decade. This increase was most pronounced in the South-Eastern Anatolian region, where the frequency rose from 21.0% to 36.9%. Conclusion: Our results indicate that there has been a marked increase in the prevalence of AR in every region in Turkey over the last 10 years. This could be related to living conditions in urban environments. Alterations in lifestyle, urban living, air pollution causing impairments in immune defense mechanisms, and other aspects of modern lifestyles may account for the increase in AR in Turkey.Item Immunological Mechanisms of Allergic Rhinitis(2021) Koycu, Alper; Yucel, Aysegul Atak; 0000-0003-1290-3509; AAF-3650-2021Allergic rhinitis is a specific immunoglobulin (Ig)E-mediated reaction developed against respiratory allergens. The main symptoms of allergic rhinitis; sneezing, runny nose, nasal congestion, itching in the nose, eyes and palate. Cells, cytokines, surface markers, transcription factors and other mediators have been revealed with the sequential removal of nasal biopsy and lavages in human and animal models exposed to allergens over the years. Respiratory allergen induces Th2 lymphocyte proliferation with a combination of cytokines including interleukin (IL)-3, IL-4, IL-5, IL-9, IL-10, and IL-13. These molecules promote allergen specific IgE, mast cells, basophils, eosinophils, adhesion molecules and chemokines. In this review, immunological mechanisms in allergic rhinitis re-examined by reviewing the current literature.Item Evaluation of the nasal septal body in pediatric patients(2021) Coban, Kubra; Ozer, Fulya; Akdogan, M. Volkan; 0000-0001-5381-6861; ABC-1809-2020Objective: The nasal septal body (NSB) is a fusiform shaped dynamic structure and the widest part of the anterior septum. It consists of a thickened septal cartilage, bone, glandular, and vasoactive structures covered by mucous membranes. Hence, it plays a role in nasal airflow and humidification. This study evaluated the size of this structure in pediatric patients with allergic rhinitis and other sinonasal pathologies. Methods: The study was conducted at the Baskent University Hospital otolaryngology department between 2015 and 2019. A total of 117 children were enrolled, including 96 patients and 21 controls. The study group was divided into 3 sub-groups: group 1, with sinonasal pathologies and allergic rhinitis; group 2, with sinonasal pathologies only; and group 3, with allergic rhinitis only. The widest horizontal part of the NSB was measured on paranasal coronal computed tomography sections. Results: The mean NSB width in the patient and control groups were 1.00 +/- 0.19 cm and 0.90 +/- 0.16 cm, respectively. The NSB was significantly larger in the patient groups compared to the controls (p=0.032). There was no significant difference among the study sub-groups in terms of NSB width (p=0.215). A significant positive correlation was found between age and NSB in the patient and control groups (r=0.366, p=0.000 and r=0.632, p=0.002, respectively). There was a significant correlation between age and NSB in group 1 and group 2 (r=0.354, p=0.015 and r=0.447, p=0.010, respectively). Conclusion: The nasal septal body is significantly larger in children with comorbid sinonasal pathologies and allergic rhinitis compared to the control group.Item Role of Single Nucleotide Polymorphisms of Mammaglobin-A Gene in Nasal Polyposis: A Case Control Study(2020) Oz, Isilay; Ozdas, Sibel; Bastimur, Sibel; Ozdas, Talih; Muz, Sami Engin; Atilla, Huntuk; Kurt, Kenan; Erbek, Selim; 0000-0003-4825-3499; 0000-0002-7380-4566; B-7604-2019; AAJ-1452-2021Objective: Nasal Polyposis (NP) is a chronic inflammatory disease and genetic factors play an important role in the pathophysiology. Mammaglobin-A (MGA) gene expression was significantly higher in patients with NP and chronic rhinosinusitis compared to normal mucosa. In the present study, we investigated the relationship between single nucleotide polymorphisms (SNPs) in the MGA gene and nasal polyposis in the Turkish population. Materials and Methods: A total of 87 patients diagnosed with NP and 60 healthy volunteers were enrolled in the study. Genotypes of MGA promoter SNPs c38C>G, c.21C>T, c55+186G>A and c.243+230A>T were determined by light SNP ASSAY after real time PCR analysis using genomic DNA samples obtained from the peripheral blood samples of all participants. Results: A total of 87 NP patients, 51 male and 36 female, with a mean age of 38.18 +/- 9.5 years were included in the study. No significant difference was determined at all positions c38C>G, c.21C>T, c55+186G>A and c.243+230A>T in nasal polyp patients compared to controls with and without allergic rhinitis (AR). Conclusion: MGA gene c38C>G, c.21C>T, c55 + 186G>A, and c.243 + 230A>T genotypes did not appear to be associated with susceptibility to NP with and without AR in our study population.Item Clinical Features of Patients with Allergic Rhinitis and Evaluation of Adenoid Hypertrophy as a Comorbidity(2019) Sancakli, Ozlem; Belverenli, H.Objective: The nasal cavity is anatomically and functionally communicates with eyes, paranasal sinuses, nasopharynx, middle ear and lower airways. Therefore, comorbid diseases such as asthma, chronic sinusitis, adenoid hypertrophy allergic conjunctivitis are common in patients with allergic rhinitis. In our study, we aimed to evaluate the frequency of comorbid diseases, clinical features of patients who referred to our outpatient clinic with allergic rhinitis. Method: The study included children aged between 3-17 years who were diagnosed with moderate to severe allergic rhinitis between September 2015 and March 2016 in our pediatric allergy outpatient clinic. Patient files were retrospectively reviewed; comorbid diseases, family history, eosinophil percentage, total IgE level and allergy test results and the number of drugs used were recorded. In the first month of the treatment, visual analog scale was used to score the symptoms. P< 0.05 was considered significant in the statistical analysis. Results: A total of 106 patients (33 female, 73 male) with respective median ages of 7.25 and 5.13 years [range: 3.0-17.0, years] were included in the study. In 84.9% of our patients, skin test was positive, and in 69.8% of the patients the symptoms persisted for more than 2 years. The most common comorbid diseases were asthma (52.8%) and adenoid hypertrophy (30.2%). In our study, the incidence of adenoid hypertrophy was higher and total IgE levels were lower in the skin test-negative group (p=0.019, p=0.027). Conclusion: In our study, the most common comorbid diseases in patients with allergic rhinitis were asthma and adenoid hypertrophy, and in the skin test-negative group adenoid hypertrophy was more frequent.Item Comparison of Acoustic and Stroboscopic Findings and Voice Handicap Index between Allergic Rhinitis Patients and Controls(2014) Koc, Eltaf Ozbal; Koc, Bulent; Erbek, Selim; 25667789Background: In our experience Allergic Rhinitis (AR) patients suffer from voice problems more than health subjects. Aims: To investigate the acoustic analysis of voice, stroscopic findings of larynx and Voice Handicap Index scores in allergic rhinitis patients compared with healthy controls. Study Design: Case-control study. Methods: Thirty adult patients diagnosed with perennial allergic rhinitis were compared with 30 age-and sex-matched healthy controls without allergy. All assessments were performed in the speech physiology laboratory and the testing sequence was as follows: 1. Voice Handicap Index (VHI) questionnaire, 2. Laryngovideostroboscopy, 3. Acoustic analyses. Results: No difference was observed between the allergic rhinitis and control groups regarding mean Maximum Phonation Time (MPT) values, Fo values, and stroboscopic assessment (p>0.05). On the other hand, mean VHI score (p=0.001) and s/z ratio (p=0.011) were significantly higher in the allergic rhinitis group than in controls. Conclusion: Our findings suggest that the presence of allergies could have effects on laryngeal dysfunction and voice-related quality of life.Item Effect of mometasone furoate nasal spray on the DNA of nasal mucosal cells(2018) Aydin, Erdinc; Akkas, Hakan; Turkoglu Babakurban, Seda; Yurtcu, Erkan; Yilmaz Ozbek, Ozlem; 0000-0001-5067-4044; 0000-0001-6864-7378; 0000-0003-4930-8164; 29714449; AAI-8856-2021; AAJ-2379-2021; AAA-2998-2021Background/aim: Allergic rhinitis (AR) is a respiratory disease caused by inflammation of the nasal mucosa. Intranasal corticosteroids (ICs) are an effective treatment for AR; however, their use has been associated with atrophy in nasal mucosae. Because DNA damage has been linked to several chronic diseases, we hypothesize that use of ICs could cause DNA damage in nasal mucosa cells, leading to mucosal atrophy and septal perforation. Materials and methods: Sixty patients with moderate or severe AR were divided randomly into two groups. Mometasone furoate (MF) and antihistamine tablets (desloratadine) were given to the study (IC) group. Physiologic saline and desloratadine were given to the control ((serum physiologic (SP)) group. Nasal irrigation fluid was taken from patients before study commencement and after 4 weeks of treatment. The comet assay was applied to detect DNA damage in nasal mucosa cells. Results: Nineteen patients were excluded, leaving a study population of 41 patients (IC group: 17 patients; SP group: 24 patients). Genotoxic damage was evaluated by comet assay. Conclusion: Treatment with MF spray for 4 weeks does not cause DNA breaks within cells in the nasal mucosa. These results could form the basis of clinical trials involving treatment with different ICs over longer treatment periods.