Tıp Fakültesi / Faculty of Medicine

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    Serum Angiostatin Levels in Patients with Behcet's Disease: Does Angiogenesis Play A Role in The Pathogenesis of Behcet's Disease?
    (2014) Keskin, D.; Keskin, G.; Inal, A.; Ozisik, L.; https://orcid.org/0000-0002-0690-2529; 25012748; J-8056-2012
    Angiogenesis plays an important role in the pathogenesis of inflammatory diseases, but the possible role of angiogenesis in Behcet's disease (BD) has not yet been studied. The aim of this study was to determine angiostatin levels in patients with BD and the role of angiogenesis in the pathogenesis of the disease. Thirty-seven patients with BD (mean age: 28.6 +/- 5.4 years, mean disease duration: 9.3 +/- 3.7 years) and 18 healthy controls were enrolled to the study. Twenty-four patients were in active and 13 patients were in inactive stage of the disease. The mean serum angiostatin level of patients with BD was 113.9 +/- 653.2 and 60.7 +/- 20.1 ng/ml in healthy controls. The mean serum angiostatin level was 142.7 +/- 43.1 ng/ml in active and 86.9 +/- 15.5 ng/ml in inactive patients with BD. Serum angiostatin levels were significantly high in patients with BD compared with healthy controls (P<0.001) and it was significantly high in active patients compared with inactive patients with BD (P<0.001). In inactive patients with BD, serum angiostatin concentrations were found to be higher compared with healthy controls (P<0.01). In active BD patients, the mean serum angiostatin level was correlated with the deep vein thrombosis (r=0.482, P=0.05), uveitis (r=0.582, P=0.01), and arthritis (r=0.492, P=0.05). According to these results; elevated serum angiostatin levels in patients with BD suggest the possible role of angiogenesis in the pathogenesis of the disease and its high levels in inactive Behcet's patients is related with the continuous activation of the disease even in the subclinical period.
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    Angiogenesis Inhibition Impairs Testicular Morphology in Experimental Left Varicocele Rat Model
    (2014) Gokhan-Kose, M.; Erdem, S. R.; Peskircioglu, C. L.; Caylak, B.; https://orcid.org/0000-0002-2491-0178; https://orcid.org/0000-0002-7537-2170; 24702910; AHI-3170-2022; AAJ-2370-2021
    Introduction: It has been reported that varicocele might promote angiogenesis. However, it is not clearly identified how angiogenesis affect testicular morphology or spermatogenic activity. The objective of the study is to investigate the effect of spironolactone, as an angiogenesis inhibitor, on the ipsilateral testis morphology in left varicocele-induced rats. Materials and methods: Twenty four adult (12-14 mo), male Wistar albino rats were randomly assigned to four groups (n=6, for each): 1. Control group, 2. Sham-operated group, 3. Experimental left varicocele group and, 4. Spironolactone (20 mg/kg/day)-treated experimental left varicocele group. Histopathological findings in rat testis were investigated. Results: Microvessel density increased in varicocele group and spironolactone inhibited angiogenesis neither by antimineralocorticoid, nor by antiandrogenic effect. However, spermatogenesis impaired in spironolactone treated varicocele group. Conclusion: Angiogenesis seems to be a protective process in varicocele. Spironolactone treatment, probably by inhibiting angiogenesis, impairs testicular morphology. (C) 2013 AEU. Published by Elsevier Espana, S.L. All rights reserved.
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    The Effect of Penile Urethral Fat Graft Application on Urethral Angiogenesis
    (2015) Cakmak, M.; Yazici, I.; Boybeyi, O.; Ayva, S.; Aslan, M. K.; Senyucel, M. F.; Soyer, T.; 0000-0002-2280-8778; 0000-0003-1505-6042; 25964198; AAQ-4836-2020; G-6569-2011; I-2260-2019; AAK-1967-2021
    Autologous fat grafts are rich in adipose-derived stem cells, providing optimal soft-tissue replacement and significant quantities of angiogenic growth factor. Although fat grafts (FG) are used in several clinical conditions, the use of FG in urethral repairs and the effects of FG to urethral repairs have not yet been reported. Objective An experimental study was performed to evaluate the effect of FG on urethral angiogenesis and tissue growth factor (GF) levels. Study design Sixteen Wistar albino, adult, male rats were allocated into two groups: the control group (CG) (n = 8) and the experiment group (EG) (n = 8). After anesthetization of all rats, 3-mm vertical incisions were made on the urethras, and then sutured with interrupted 5/0 vicryl sutures. The operations were performed under a stereo dissecting microscope under magnification (x20). In the CG, no additional procedure was performed. In the EG after the same surgical procedure, 1 mm(3) FG was removed from the inguinal region by sharp dissection with a knife. The grafts were trimmed to 1 x 1 mm dimensions on millimeter paper. The FGs were placed on the repaired urethras. The skin was then closed. Samples from urethral and penile skin were taken 21 days after surgery in both groups. Density and intensity of staining with vascular-endothelial GF (VEGF), VEGF-receptor, and endothelial-GF receptor (EGFR) in the endothelial and mesenchymal cells of the penile urethral vessels were immunohistochemically evaluated. Data obtained from immunohistochemical evaluations were analyzed with SPSS 15.0. The P-values lower than 0.05 were considered as significant. Results Density of VEGF staining was significantly decreased in the vascular endothelium of the EG compared to the CG (P < 0.05). Density of the EGFR staining was significantly decreased in the vascular endothelium of the EG compared to the CG (P < 0.05) (Table). Intensity of VEGF, VEGF-R and EGFR staining was not significantly different between the two groups. There were no significant differences between groups regarding to VEGFR staining and mesenchymal examination. Discussion Decreased density was found in the VEGF staining in the vascular endothelium. This could be explained by the day that the tissues were harvested or because autologous fat grafts might cause decreased growth factor levels, which is contrary to the literature data. Conclusion Fat grafting has an immunohistochemical effect on the growth factor levels that are related to angiogenesis after urethral repair. It is difficult to make a firm conclusion about the role of fat grafting on urethral healing. Therefore, future studies are needed to see if FG can be used as an alternative to other procedures in order to avoid complications.