Tıp Fakültesi / Faculty of Medicine
Permanent URI for this collectionhttps://hdl.handle.net/11727/1403
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Item Effect of Rat Urine on Ileum Muscle Layer of Bladder Reconstruction: An Experimental Study(2018) Ersoz, Dilsah; Kirnap, Mahir; Ozdemir, B. Handan; Bacanli, Didem; Haberal, Mehmet; https://orcid.org/0000-0002-7528-3557; https://orcid.org/0000-0002-3462-7632; AAH-9198-2019; X-8540-2019; AAJ-8097-2021Item Bridging Technique of Bile Duct Anastomosis Using An Expanded Polytetrafluoroethylene (Eptfe) Graft in A Porcine Model(2016) Haberal, Mehmet; Ozdemir, Handan; Bacanli, Didem; Ozcay, Necdet; Ersoy, Zeynep; Torgay, Adnan; https://orcid.org/0000-0002-3462-7632; https://orcid.org/0000-0003-0767-1088; AAJ-8097-2021; AAF-3066-2021Item Prevention of Vascular Stricture By Using An Expanded Polytetrafluoroethylene (Eptfe) Graft in A Porcine Model: Preliminary Study(2016) Haberal, Mehmet; Ozdemir, Handan; Ozcay, Necdet; Bacanli, Didem; Ersoy, Zeynep; Torgay, Adnan; https://orcid.org/0000-0002-3462-7632; https://orcid.org/0000-0003-0767-1088; https://orcid.org/0000-0002-6829-3300; AAJ-8097-2021; AAF-3066-2021; AAJ-5221-2021Item Technique of Ileobladder and Kidney Transplant in Rats and Pigs(2018) Haberal, Mehmet; Kirnap, Mahir; Gokce, Oruc N.; Bacanli, Didem; Ersoy, Zeynep; Bayzakov, Mirbek; Torgay, Adnan; Ozdemir, Handan; Erdem, Remzi; 0000-0003-0767-1088; 0000-0002-9678-7818; 0000-0002-7528-3557; 0000-0002-6829-3300; 0000-0002-3462-7632; 0000-0002-7537-2170; 29409436; AAF-3066-2021; AAH-9198-2019; AAQ-8259-2021; X-8540-2019; AAJ-5221-2021; AAJ-8097-2021Objectives: Kidney transplant is the best choice for treatment of patients with advanced chronic renal disease. However, small, poorly compliant, and unstable bladders can result in major problems for patients. Here, we aimed to develop and evaluate a new ileobladder model. Materials and Methods: Fifteen rats (250-300 g) and 5 pigs (similar to 100 kg) were cared for according to institutional and published guidelines. After general anesthesia, laparotomy was done through midline incision. Ileal loops were prepared for ileobladder. After cystectomy (0.5 cm above the trigone in rats, 1 cm above the trigone in pigs), anastomoses were done between antimesenteric sides of ileal loops and bladder remnant with 6/0 Prolene suture. Three other pigs received simultaneous renal transplant. Results: One rat died on day 1 postsurgery from multiorgan hemorrhage. Two rats survived for 5 days, 3 rats for 7 days, and 3 rats for 11 days; 6 rats were killed for pathologic evaluation after 3 months. One pig survived for 22 days and 1 for 9 days. Of the 3 pigs that received a simultaneous renal transplant, 2 pigs were alive and doing well 80 and 72 days after surgery with normal urinary discharge (1 pig was killed for pathologic evaluation after 3 days). When ileobladder was opened, complete recovery of the anastomosis line was observed. Pathologic examination of the anastomosis sites reported a normal healing process with moderate inflammation and the muscular wall of the intestine showed hypertrophia that nearly reached the size of the bladder muscularis propria. Conclusions: Although we had some complications because no draining procedure was used, in terms of technique, our new ileobladder model is promising for providing functional bladder volume. A larger scale series in the clinical setting is planned. This technique can be useful for small bladders and bladder physiology disorders.Item Preliminary Experimental Model for "Ileobladder" and Renal Transplant in Rats and Pigs(2017) Haberal, Mehmet; Kirnap, Mahir; Gokce, Oruc Numan; Bacanli, Didem; Ersoy, Zeynep; Bayzakov, Mirbek; Torgay, Adnan; Ozdemir, Handan; 0000-0002-3462-7632; 0000-0002-6829-3300; 0000-0003-0767-1088; 0000-0002-6829-3300; 0000-0002-7528-3557; AAJ-8097-2021; AAH-9198-2019; AAJ-5221-2021; AAF-3066-2021; AAJ-5221-2021; X-8540-2019Item Effect of Rat Urine on Ileum Muscle Layer of Bladder Reconstruction: An Experimental Study(2018) Ersoz, Dilsah; Kirnap, Mahir; Ozdemir, B. Handan; Bacanli, Didem; Haberal, Mehmet; 0000-0002-7528-3557; 0000-0002-3462-7632; AAH-9198-2019; X-8540-2019; AAJ-8097-2021Item Antiproliferative and anti-apoptotic effect of astaxanthin in an oxygen-induced retinopathy mouse model(2019) Kucukoduk, Ali; Helvacıoglu, Fatma; Haberal, Nihan; Dagdeviren, Atilla; Bacanli, Didem; Yilmaz, Gursel; Akkoyun, Imren; 0000-0001-8990-8282; 30851776; P-2877-2014Objective: To evaluate the impact of intravitreal (IV) and intraperitoneal (IP) astaxanthin (AST) injections on neovascular development (ND), retinal morphology, and apoptotic activity in a C57BL/6J mouse model with hyperoxia-induced retinopathy (HIR). Design: C57BL/6J mouse model. Methods: Two negative control groups (n = 6 each; one of which received IV sterile dimethyl sulfoxide [DMSO]) of C57BL/6J-type mice were exposed to room air. The HIR groups included 36 C57BL/6J-type mice exposed to 75% +/- 2% oxygen from postnatal day (PD) 7 to PD 12. On PD 12, these mice were randomized into 6 groups (n = 6 each): 2 HIR control groups (one of which received IV-DMSO), 2 IV-AST groups (10 and 100 mu g/mL), and 2 IP-AST groups (0.5 and 5 mg/kg). We measured ND by counting neovascular tufts in cross sections and examined histological, ultrastructural changes via light and electron microscopy. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated nick end-labeling. Results: No ND was detected in the negative control groups. ND levels were not significantly different between high- and low-dose AST for either means of administration. However, ND levels were significantly lower in the AST groups, regardless of delivery, compared to the control groups. The means of delivery (IP versus IV) also yielded significant differences in ND. The incidence of mitochondrial dysmorphology and apoptosis were lower in groups receiving AST. Conclusions: AST seems to suppress ND and has anti-apoptotic activity in the HIR mouse model.Item EVALUATION OF NEW BASKENT UNIVERSITY PRESERVATION SOLUTION FOR LIVER, KIDNEY AND INTESTINE GRAFT DURING COLD ISCHEMIA: PRELIMINARY EXPERIMENTAL ANIMAL STUDY(2019) Haberal, Mehmet; Kirnap, Mahir; Erdem, Remzi; Ozdemir, B. Handan; Lux, K. Michael; Bacanli, Didem; AAH-9198-2019Item EFFECT OF NEW BASKENT UNIVERSITY ORGAN-PRESERVATION SOLUTION ON THE ACTIN CYTOSKELETON REARRANGEMENT AND APOPTOSIS OF RENAL TUBULAR CELLS: COMPARISON WITH OTHER PRESERVATION SOLUTIONS(2019) Haberal, Mehmet; Ozdemir, B. Handan; Kirnap, Mahir; Erdem, Remzi; Lux, K. Michael; Bacanli, Didem; AAH-9198-2019Item Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study(2019) Haberal, Mehmet; Kirnap, Mahir; Erdem, S.Remzi; Ozdemir, B.Handan; Lux, K. Michael; Bacanli, Didem; 31145052Objectives: Organ damage due to long cold ischemia time remains a hurdle in transplantation. In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions. Materials and Methods: BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid. In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation. In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other. Results: In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution. At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01). The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01). In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage. Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups. Conclusions: Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS. BUPS can be a costeffective perfusion solution in transplantation.