Başkent Üniversitesi Yayınları
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Item The Impact of Fat Rich Enteral Feeding on the Histopathological Changes of Liver in Third Degree Burns: An Experimental Study(Başkent Üniversitesi, 2022-12) Santiago J. Santelis; Ayse Ebru Abali; Gonca Ozgun; Binnaz Handan Ozdemir; Burak Ozkan; Mehmet HaberalABSTRACT OBJECTIVES: This study aimed to describe the histo-pathological changes in the liver secondary to severe burns and to investigate the effects of a fat-rich diet during the initial 7 days post burn in a rat model. MATERIALS & METHODS: We randomly assigned 21 Wistar-albino rats into 3 groups: sham group, burn + standard-diet group, and burn + fatty-diet group. A full-thickness burn wound was induced on the dorsa of the animals in both burn groups. Seven days after sham procedures/burn induction, hepatic tissues were examined under light microscopy; hepatocyte size, portal-triad size, and binuclear hepatocyte numbers were calculated. RESULTS: The mean number of binuclear hepatocytes was higher in burn + standard-diet group than in the sham group. Mean hepatocyte area and mean portal-triad area were narrower in the burn + standard-diet group compared with the sham group (P < .05). Mean hepatocyte area and mean portal-triad area in the burn + fatty-diet group were closer to those shown in the sham animals, and binuclear hepatocytes number in the burn + fatty diet group was slightly higher than in the burn + standard diet group(P > .05). CONCLUSIONS: Liver damage was mildly influenced by fat-rich enteral feeding in the 7 days postburn. Further studies must focus on dynamic feeding protocols designed according to the phases involved in the burn trauma instead of seeking a stable suitable protocol.Item Effects of a Fat-Rich Diet in the Pancreas of Rats During the Acute Phase of Burns(Başkent Üniversitesi, 2022-06) Neslihan Başçıl Tütüncü; Ayse Ebru Abali; Santiago J. Santelis; Özlem Turhan İyidir; B. Handan Ozdemir; Gonca Ozgun; Nilüfer Bayraktar; Meriç Çolak; Mehmet HaberalABSTRACT OBJECTIVES: Oversupply of nutrients overstimulates beta cells in standard conditions, and severe burn injuries increase the metabolic needs. In this study, we investigated the effects of fat-rich nutrients on the endocrine pancreas during the acute phase of severe burns.Item Impact of Severe Burns on Pancreatic Islets: An Experimental Model in Rats(Başkent Üniversitesi, 2022-03) Santiago J. Santelis; Ayse Ebru Abali; Gonca Ozgun; B. Handan Ozdemir; Neslihan Basci Tutuncu; Mehmet HaberalABSTRACT OBJECTIVES: Severe burn victims experience a systemic inflammatory response and a hypermetabolic response that can generate adverse effects on many distant organs and systems. Our aim in this study was to describe the histopathological changes in the pancreatic islets secondary to severe burns in an experimental animal model. MATERIALS & METHODS: Fourteen Wistar albino rats were randomly divided into 2 groups: the sham group and the burn group. A full-thickness burn model was designed to induce a burn of 25% total body surface area. Seven days after burn induction and sham procedure, pancreatectomy was performed. Pancreatic tissues were examined under light microscopy, and islet size and cellularity were calculated. RESULTS: The histopathologic examination was unremarkable, but the mean number of islets per pancreatic tissue was lower in the burn group than in the sham group. We observed a significant difference in the mean number of cells per one islet between the 2 groups, with the cell count higher in the burn group (P < .05). CONCLUSIONS: During the acute phase of burn injury in rats, we observed a decrease in the number of pancreatic islets with remarkable hypercellularity. Further studies are needed to determine the histological and cellular basis of these changes.