Fakülteler / Faculties
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Item Comparison of The Plaque Regrowth Inhibition Effects of Oil Pulling Therapy with Sesame Oil or Coconut Oil Using 4-Day Plaque Regrowth Study Model: A Randomized Crossover Clinical Trial(2023) Sezgin, Yasemin; Memis Ozgul, Betul; Maras, Muhammed Enes; Alptekin, Nilgun Ozlem; 34124840; AAC-5931-2020; AAZ-1977-2021Objectives The aim of this study was to compare the plaque-inhibiting effects of oil pulling therapy with sesame oil or coconut oil using 4-day plaque regrowth study model. Methods This clinical observer-masked, randomized, crossover designed study involved 24 participants. The participants received professional prophylaxis in the preparatory period and after that subjects started to use the allocated mouthrinse (coconut oil or sesame oil). On day 5, periodontal clinical parameters including plaque index (PI), gingival index (GI), stain index (SI) and bleeding on probing (BOP) were recorded. Subjects underwent a 14-day wash out period and then used the other mouthrinse for 4 days. Results Oil pulling therapy with coconut oil or sesame oil exhibited similar plaque regrowth inhibition (PI = 1.60 +/- 0.28 and 1.49 +/- 0.22, for oil pulling with coconut oil and sesame oil, respectively) and tooth staining (SI = 0.20 +/- 0.11 and 0.21 +/- 0.09, for oil pulling with coconut oil and sesame oil, respectively.) In addition, GI and BOP were similar in both groups (GI = 0.61 +/- 0.19 and 0.69 +/- 0.16; BOP = 0.09 +/- 0.24 and 0.03 +/- 0.03 for oil pulling with coconut oil and sesame oil, respectively). Conclusions Oil pulling therapy with coconut or sesame oil showed similar results in terms of plaque regrowth inhibition and tooth staining. According to the present results, both coconut oil and sesame oil can be used for oil pulling therapy with the aim of plaque regrowth inhibition.Item Interleukin-1 Receptor Antagonist Gene Polymorphism, Adverse Pregnancy Outcome and Periodontitis in Turkish Women(2015) Kayar, Nezahat Arzu; Alptekin, Nilgun Ozlem; Erdal, Mehmet Emin; 0000-0002-6191-2930; 0000-0003-4104-6462; 0000-0002-2325-2900; 26445016; F-9241-2015; G-1816-2014; AAA-7336-2021Objective: The aim of this study was to determine associations between interleukin (IL)-1A (+4845), IL-1B (+3954), and IL-1 receptor antagonist (RN) variable number tandem repeat polymorphisms and adverse pregnancy outcomes and periodontitis in a Turkish women. Design: A total of 156 patients, including 64 women with normal birth outcome (NB) and 92 women with preterm/low birth weight outcome (PLBW) were included in this case-control study. Within 24 h after labor, maternal demographic characteristics and clinical periodontal parameters were recorded. The distribution and genotype frequencies of IL-1 were analyzed with polymerase chain reaction-restriction fragment length polymorphism assay. Statistical analyses were carried out for clinical periodontal parameters, genotype frequencies, and to identify explanatory variables for PLBW. Results: PLBW was associated with maternal age (p < 0.05), irregular prenatal care (p < 0.001), previous PLBW (p < 0.05), and antibiotic use during pregnancy (p < 0.05). Measurements of probing depth and clinical attachment level (CAL) were significantly higher in the PLBW group than in the NB group (p < 0.001). PLBW was associated with IL-1RN allele 2 (p < 0.001). Moreover, stepwise logistic regression analysis showed that CAL (OR 1.39, 95% CI: 1.04-1.85) and IL-1RN polymorphism (OR 7.92, 95% CI: 2.76-22.79), previous PLBW (OR 5.01, 95% CI: 1.08-23.17), age (OR 1.22, 95% CI: 1.04-1.44) were predictors found to increase the risk of PLBW (p < 0.05). There was a negative association between PLBW and regular prenatal care, total number of births, use an antibiotic during pregnancy period (p < 0.05). Conclusion: Our study showed that, IL-1RN allele 2, periodontal disease characterized with clinical attachment loss, previous PLBW and age could be an important risk factors for PLBW. (C) 2015 Elsevier Ltd. All rights reserved.Item Frequency of MCP-1 (rs1024611) and CCR2 (rs1799864) and Its Effect on Gene Expression Level in Patients with Agp(2017) Gunpinar, Sadiye; Alptekin, Nilgun Ozlem; Ucar, V. Betul; Acar, Hasan; 0000-0003-4104-6462; 28458180; G-1816-2014Objective: To identify the genetic risk markers of aggressive periodontitis (AgP), researchers focus on genetic components that regulate the immune response. Therefore the purpose of this study was to investigate genetic impact of monocyte chemoattractant protein (MCP)-1-2518 A/G and CC chemokine receptor 2 (CCR2) 190 G/A gene polymorphisms on AgP susceptibility and the effect of this polymorphism on MCP-1 gene expression in patients with AgP. Material and methods: A total of 215 subjects, 108 AgP and 107 periodontally healthy (H) were recruited in this cross-sectional study (NCT02817568). Gene polymorphisms of MCP -1-2518 A/G and CCR2-190 G/A were analyzed by a standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. MCP-1 messenger (m) RNA expression was measured using quantitative real-time (RT)-PCR in peripheral blood leukocytes from 26 AgP and 16 H controls. Threshold cycles (C-t) values were obtained from the RT-PCR analysis based on SYBR Green detection and data was normalized via triangle C-t. Results: There were no differences between AgP and H groups with regard to MCP-1 and CCR2 genotype distribution and allele frequencies (p > 0.05). In contrast, the MCP-1 mRNA expression levels were higher in homozygous "AA" control subjects than having G(+) genotype and AA homozygous AgP patients. Conclusions: It can be concluded that MCP-1 and CCR2 polymorphisms are not associated with AgP in Turkish population. Although in AgP patients, there was AA genotype with MCP -1 mRNA expression it can be speculated that gene expression levels in peripheral blood may not reflect the cytokine/chemokine levels of local tissues.Item Frequency of MCP-1 (Rs1024611) and CCR2 (Rs1799864) Gene Polymorphisms, and Its Effect on Gene Expression Level in Patients with Agp(2017) Gunpinar, Sadiye; Alptekin, Nilgun Ozlem; Ucar, V. Betul; Acar, Hasan; https://orcid.org/0000-0003-4104-6462; 28458180; G-1816-2014Objective: To identify the genetic risk markers of aggressive periodontitis (AgP), researchers focus on genetic components that regulate the immune response. Therefore the purpose of this study was to investigate genetic impact of monocyte chemoattractant protein (MCP)-1-2518 A/G and CC chemokine receptor 2 (CCR2) 190 G/A gene polymorphisms on AgP susceptibility and the effect of this polymorphism on MCP-1 gene expression in patients with AgP. Material and methods: A total of 215 subjects, 108 AgP and 107 periodontally healthy (H) were recruited in this cross-sectional study (NCT02817568). Gene polymorphisms of MCP -1-2518 A/G and CCR2-190 G/A were analyzed by a standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. MCP-1 messenger (m) RNA expression was measured using quantitative real-time (RT)-PCR in peripheral blood leukocytes from 26 AgP and 16 H controls. Threshold cycles (C-t) values were obtained from the RT-PCR analysis based on SYBR Green detection and data was normalized via triangle C-t. Results: There were no differences between AgP and H groups with regard to MCP-1 and CCR2 genotype distribution and allele frequencies (p > 0.05). In contrast, the MCP-1 mRNA expression levels were higher in homozygous "AA" control subjects than having G(+) genotype and AA homozygous AgP patients. Conclusions: It can be concluded that MCP-1 and CCR2 polymorphisms are not associated with AgP in Turkish population. Although in AgP patients, there was AA genotype with MCP -1 mRNA expression it can be speculated that gene expression levels in peripheral blood may not reflect the cytokine/chemokine levels of local tissues.Item Peri-Implant Crevicular Fluid And Serum Levels Of Soluble ST2 In Peri-Implant Diseases: A Pilot Study(2023) Ozgur, Engin; Topcu, Deniz Ilhan; Bayraktar, Nilufer; Alptekin, Nilgun Ozlem; https://orcid.org/0000-0002-7911-198X; https://orcid.org/0000-0002-1219-6368; https://orcid.org/0000-0002-7886-3688; https://orcid.org/0000-0003-4104-6462; 36504319; E-3717-2019; Y-8758-2018; G-1816-2014Background and ObjectiveSoluble ST2 (sST2) is a current biomarker of cardiovascular disease. It is used to predict susceptibility to cardiovascular diseases and to analyze their prognosis. Serum sST2 level increases in inflammatory diseases such as periodontitis. However, the level of sST2 in peri-implant diseases and crevicular fluid has not been investigated yet. Thus, the aim of this cross-sectional study is to analyze the level of sST2 in peri-implant health and diseases. MethodsSixty-nine participants were divided into 3 groups as peri-implant health (PH), peri-implant mucositis (PM), and peri-implantitis (P-I). Peri-implant crevicular fluid (PICF) and serum samples were collected from each participant. The levels of sST2 and IL-6 in PICF and sST2, IL-6, and CRP in serum were compared between the groups. Pocket depth (PD), modified bleeding index (mBI), modified plaque index (mPI), keratinized mucosa index (KTW), and gingival/mucosal recession (REC) were recorded as clinical parameters. Biomarkers in the serum and PICF were analyzed by ELISA kit. ResultsSixty-nine patients were included in the study. The differences in the following parameters were statistically significant between groups: age (p = .009), implant function time (p = .027), PD (p < .001), mBI (p < .001), mPI (p < .001), and KTW (p = .043). The PICF volume of P-I and PM groups were statistically higher than PH (p < .001). The amount of sST2 in P-I and PM groups were higher than PH (p = .043). Serum CRP was higher in the P-I group than in other groups (p = .034). There were no significant differences in serum sST2 (p = .247) and IL-6 (p = .110) levels between groups. ConclusionThe PICF levels of sST2 were significantly higher in PM and P-I groups compared to the healthy group. However, no significant difference was observed between the groups in terms of serum sST2 level.Item The Effects of Non-Surgical Periodontal Therapy on Neutrophil Elastase and Elastase Alpha-1 Proteinase Inhibitor Levels in GCF in Periodontitis Patients with or Without Acute Coronary Syndrome(2021) Kayar, Nezahat Arzu; Ustun, Kemal; Gozlu, Muammer; Haliloglu, Seyfullah; Alptekin, Nilgun Ozlem; 33687554Objective Periodontitis may contribute to vascular damage, resulting in the destabilization of atherosclerotic plaque leading to acute coronary syndrome (ACS). In this study, we explored the effect of non-surgical periodontal treatment (NSPT) on cardiovascular blood biomarkers and gingival crevicular fluid (GCF) neutrophil elastase (NE) and alpha 1-proteinase inhibitor (alpha-1PI) levels in periodontitis (P) participants with and without ACS. Materials and methods Medical and dental examinations were performed to diagnose ACS and periodontitis, respectively. Seventeen patients with diagnosis of ACS and periodontitis were included in this study, as a test group (group ACS). Twenty-six age and sex-matched control patients with periodontitis (group P) were otherwise systemically healthy. Both groups received NSPT. Plasma levels of cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL), C-reactive protein (CRP), GCF NE activity, GCF alpha(1)-PI levels, and GCF NE/alpha 1-PI rates were measured at baseline, at1(st) and 3(rd) months after NSPT. Results GCF NE activity/time (mu U/30s) decreased significantly at 3(rd) month compared to baseline values in the Group P after NSPT. First and 3(rd) months after NSPT, in the Group P GCF alpha(1)-PI activity/time (pg/30s) was significantly higher than the Group ACS. Moreover GCF NE/alpha(1)-PI rates decreased significantly compared to baseline values at 1(st) and 3(rd) months after NSPT in the group P. Conclusion NSPT yields decrease in NE/alpha(1)-PI rates. NE and its possible interactions with alpha(1)-PI may play a crucial role in both periodontitis and ACS. GCF alpha 1PI activity/time (U/30s) can be a potential biomarker in management of periodontitis associated with ACS.Item Evaluating Well-being Among Dental Students Using the Warwick-Edinburgh Mental Well-being Scale and the Relationship Between Mental Well-being and Socio-demographic Findings(2021) Cetin, Mehtap Bilgin; Sezgin, Yasemin; Akinci, Seray; Alptekin, Nilgun Ozlem; 0000-0003-4104-6462; G-1816-2014Objective: The present study aimed to measure mental well-being among dental students using the Warwick-Edinburgh Mental Well-being Scale (WEMWBS) and to investigate factors affecting well-being. Materials and Methods: Baskent University Faculty of Dentistry students were invited to participate in the study in 2019. The participants were asked to complete the WEMWBS and a questionnaire describing their demographic details, social background and lifestyle habits. Results: A total of 268 dental students (68% females) participated in the survey, with a response rate of 85%. The overall WEMWBS score of the study population was 48.18 +/- 9.6. Statistically significant differences in WEMWBS scores were detected between first- and fourth-year (p<0.001) students and between fourth- and fifth-year students (p<0.001). Male students (p<0.001) whose first choice was to attend the school of dentistry (p=0.024) and who pursued hobbies (p=0.001) or regular sports activities (p=0.001) had significantly higher WEMWBS scores than those who did not. Dental students' smoking status (p=0.630), living accommodation (p=0.71) or parents' education level did not affect WEMWBS scores significantly. Conclusion: The mental well-being of dental students was influenced by their year of study, gender, first choice of school and pursuit of hobbies or regular sports activities. Factors that may affect the mental well-being of students should be carefully considered, and strategies should be implemented to monitor and promote well-being during the students' academic career.Item Immune responses in women with periodontitis and preterm low birth weight: Levels of CD4+and CD8+T cells in gingival granulation tissue(2020) Kayar, Nezahat Arzu; Celik, Ilhami; Alptekin, Nilgun Ozlem; 0000-0003-4104-6462; 31542704; G-1816-2014Objective: Preterm Low-Birth-Weight (PLBW) is frequently associated with periodontal disease. However, the mechanism is still unknown. The present study was performed to examine the possible link between periodontal infections and PLBW in post-partum women utilizing clinical parameters and CD4+ and CD8 + T lymphocytes ratio in gingival granulation tissue. Materials: The tissues used in this study consisted of 35 gingival granulation tissue biopsies from 35 mothers of healthy infants (HTBW), 35 biopsies of gingival granulation tissue from 35 mothers of PLBW within one month postpartum and gingival tissue biopsies from 7 control individual with no periodontal disease (HC). CD4 + and CD8 + T lymphocyte ratios in a unit area of the gingival granulation tissue were determined by hystometrically. Statistical analysis was performed by using Kruskal-Wallis and Mann-Whitney U tests. Results: CD8 + T lymphocytes were more prevalent in the PLBW group than in the HTBW and HC group (P < 0.05). The CD4 + /CD8 + ratio in the PLBW group was lower than those of the other groups (p < 0.05). There were no statistically significant differences in CD4 + T lymphocytes counts between all groups (P > 0.05). Conclusion: Within the limits of this study it can be concluded that CD8 + T lymphocytes in gingival tissue may play important roles in the pathogenesis of periodontitis and PLBW.Item Systemic treatment with alpha-tocopherol and/or sodium selenite decreases the progression of experimental periodontitis(2020) Bas, Nurgul; Kayar, Nezahat Arzu; Baba, Z. Fusun; Avunduk, Mustafa Cihat; Haliloglu, Seyfullah; Alptekin, Nilgun Ozlem; 32986166Objective To investigate the effects of sodium selenite (Se) and/or alpha-tocopherol (alpha T) applications on the alveolar bone loss (ABL), the number of gingival collagen fibers, inducible nitric oxide synthase (iNOS)+ and CD95+ cell numbers, and serum cytokine concentrations in experimental periodontitis in rats. Materials and methods Forty Sprague Dawley rats were divided into four groups of ten as follows: group A: Se group, group B: alpha T group, group C: Se and alpha T combined group, and group D: control group (intraperitoneal (IP) saline injection applied). Using the image analysis method in the connective tissue under the connective epithelium, the numbers of iNOS, CD95 positive cells, and collagen fibers were counted. ELISA kits were used to test the concentrations of serum interleukin (IL)-1 beta, IL-6, and IL-4. Results The combination of Se and alpha T (group C) suppressed ABL compared with the control group (group D) (P< 0.05). In group A (Se), the number of iNOS+ cells was smaller than in group D (P< 0.05). Conclusion Se has been concluded to inhibit inflammation of the gum due to iNOS. Se and alpha T can have a remarkable important role in preventing alveolar bone loss, and particularly in combination.Item Evaluating the Effects of a Topical Preparation with Dexpanthenol, Silbiol, Undecylenic Acid, and Lidocaine on Palatal Mucosa Wound Healing in a Rat Model(2019) Sezgin, Yasemin; Cetin, Mehtap Bilgin; Bulut, Sule; Alptekin, Nilgun Ozlem; Borcek, Pelin; 0000-0003-4104-6462; 30322831; G-1816-2014; AAC-5448-2020; AAC-5931-2020Background: Postoperative complications occur after periodontal plastic surgeries, but an ideal treatment to overcome them has not been found yet. Aims: To evaluate the effects of topically applied Oral-norm gel on the healing of excisional wounds. Study Design: Animal experiment. Methods: Excisional wounds with a diameter of 3 mm were made in the center of the palatal mucosa of 63 Sprague Dawley rats. Seven animals were sacrificed at time 0. The remaining rats were divided into two groups: a test group in which the topical Oral-norm gel was applied three times a day and a control group in which nothing was applied. Seven animals in each group were sacrificed at 3, 7, 14, and 21 days. Mean wound surface area was measured photographically, while wound healing and width were evaluated microscopically. Results: The mean wound surface area decreased significantly after 3 days in both groups (p<0.001). Between days 3 and 7, the mean wound surface area decreased from 6.62 (2.85) to 0.83 (1.62) mm 2 in the control group and 5.07 (0.88) to 1.42 (1.67) mm 2 in the test group. The wound width decreased significantly on day 7 in both groups (p<0.001), with no further changes by day 14. Both groups had a significant increase in inflammation and vascularization on day 3 (p<0.001), with a reduction thereafter. No significant differences in macroscopic and microscopic measurements were observed between the groups at any time point (p>0.05). Conclusion: The Oral-norm gel has no positive healing effects in the palatal mucosa of rats.