Fen Edebiyat Fakültesi / Faculty of Letters and Science

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    Residual Protein Analysis By SDS-PAGE In Clinically Manufactured BM-MSC Products
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-05-11) Kilic, Pelin; Karabudak, Sema; Cosar, Begum; Savran, Busra Nigar; Yalcin, Merve
    Residual substances that are considered hazardous to the recipient must be removed from final cellular therapeutic products manufactured for clinical purposes. In doing so, quality rules determined by competent authorities (CAs) for the clinical use of tissue- and cell-based products can be met. In our study, we carried out residual substance analyses, and purity determination studies of trypsin and trypsin inhibitor in clinically manufactured bone marrow-derived mesenchymal stromal/stem cell products, using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. Despite being a semiquantitative method, SDS-PAGE has several benefits over other methods for protein analysis, such as simplicity, convenience of use, and affordability. Due to its convenience and adaptability, SDS-PAGE is still a commonly used method in many laboratories, despite its limits in dynamic range and quantitative precision. Our goal in this work was to show that SDS-PAGE may be used effectively for protein measurement, especially where practicality and affordability are the major factors. The results of our study suggest a validated method to guide tissue and cell manufacturing sites for making use of an agreeable, accessible, and cost-effective method for residual substance analyses in clinically manufactured cellular therapies.
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    What Drives Mating Effort: Fear of Singlehood, Relationship Status, and Self-Esteem
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-04-27) Apostolou, Menelaos; Tekes, Burcu; Kagialis, Antonios
    Purpose Attracting and maintaining a romantic partner requires considerable effort. In the current study, we aimed to test the hypothesis that fear of singlehood is a primary factor driving individuals to allocate scarce resources in the mating domain.Methods We conducted a close-ended survey with a sample of 990 Greek and Turkish-speaking participants.Results We found that that a stronger fear of singlehood was associated with higher mating effort. Moreover, voluntarily single participants experienced lower fear of singlehood compared to other categories of singles and individuals in intimate relationships. Additionally, higher self-esteem was associated with lower fear of singlehood. We also identified a significant indirect effect of relationship status and self-esteem on mating effort through fear of singlehood. Specifically, involuntarily single individuals tended to exert more mating effort due to heightened fear of singlehood compared to those in other relationship status categories. Furthermore, higher self-esteem was associated with reduced mating effort, as it was associated with lower fear of singlehood. These findings held true across both the Greek and Turkish samples.Conclusion Relationship status and self-esteem play a role in mating effort through fear of singlehood.
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    Biochemical And Molecular Analyses Of Insecticide Resistance In Greenhouse Populations Of Bemisia Tabaci (Hemiptera: Aleyrodidae) In Türkiye
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-04-04) Erdogan, Cem; Toprak, Umut; Gurkan, M. Oktay
    The sweet potato/cotton whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is one of the most destructive vegetable pests in greenhouses in Turkiye. While the preferred method of control primarily relies on insecticides such as neonicotinoids, organophosphates and pyrethroids, their intensive use has caused whiteflies to rapidly become resistant. Samples were collected from 13 Turkish B. tabaci greenhouse populations. Bioassays from the Marmara Region identified as high as a 53-fold increase in resistance to chlorpyrifos-ethyl, a 303-fold increase to imidacloprid and a 282-fold increase to acetamiprid. Bioassays in the Central Anatolia Region reported a 76-fold increase in resistance to deltamethrin and-15-fold increase to thiamethoxam. Most of the populations showed cross-resistance for neonicotinoids. All collected B. tabaci populations were determined to be MEAM 1 (also referred to as biotype B) for diagnostic band E0.14. The E0.14 esterase variant that helps to diagnose biotype B insects was found in all individuals from field populations. Almost all populations showed higher non-specific esterase, glutathione-S-transferase (GST) and monooxygenase activity when compared to susceptible SUD-S population. AChE insensitivity assays for individual B. tabaci suggest a target-site modification as a mechanism of resistance to chlorpyrifos-ethyl. This is the first report of AChE variants identified based on their sensitivities to chlorpyrifos ethyl-oxon and pirimicarb in Turkish B. tabaci populations. Fixed ace mutations in the target-site of organophosphates and pyrethroids were identified in six populations. Resistance to organophosphates and neonicotinoids were at least partially related to both ace mutations and insensitive AChE and monooxygenase activities, respectively. The results will help develop effective resistance management programs of B. tabaci in Turkiye.
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    The Influence of Gender Roles on Eating Attitudes: A Study Among Female College Students Abstract
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-10-12) Ozel, B.; Alici, Y. Hosgoren; Kocak, O. M.; Ceran, S.
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    The Influence of Gender Roles on Eating Attitudes: A Study Among Female College Students
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-10-12) Ozel, B.; Alici, Y. Hosgoren; Kocak, O. M.
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    Is There A Relationship Between The Severity Of Disease İn Major Depressive Disorder Patients And Dry Eye Disease?
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-04-05) Gokce, Gizem Dogan; Metin, Merve
    PurposeTo investigate dry eye disease (DED) in newly diagnosed patients with depressive disorder (MDD). MethodThis observational study included 48 MDD patients in Group 1 and 20 healthy controls in Group 2. Psychiatric and ophthalmic examinations, Beck Depression Inventory (BDI), Ocular Surface Disease Index (OSDI), Schirmer's test, tear breakup time (TBUT), Meibomian gland dysfunction (MGD), and ocular staining were conducted. The results were statistically compared. ResultsThe participants, comprising 32 men and 36 women, had a mean age of 31.08 +/- 11.7 years (18-64 years). Group 1 had a mean BDI score of 30.87 +/- 8.56, while Group 2 had a score of 1.3 +/- 1.3 (p < 0.001). In Group 1, 28 patients were diagnosed with DED, whereas in Group 2, six subjects were diagnosed with DED. The mean Schirmer's results in Group 1 and Group 2 were (mm/5 min) 10.87 +/- 2.44 and 12.70 +/- 2.3, respectively, and were significantly lower in Group 1 (p < 0.001). The mean OSDI scores in Group 1 (34.95 +/- 15.8) were significantly higher compared to Group 2 (3.2 +/- 3.1) (p < 0.001). There was no significant difference in mean TBUT between Group 1 (9.41 +/- 2.6 s) and Group 2 (9.8 +/- 0.61 s) (p > 0.05). Significant correlations were found between BDI scores and Schirmer's results as well as OSDI scores (p < 0.05, p = 0.02, respectively). No statistically significant correlations were found between BDI scores and TBUT or MGD (p > 0.05). ConclusionDED was found to be more prevalent in the MDD group. The severity of MDD and DED, as indicated by BDI, OSDI, and Schirmer's results, was found to be correlated. It was observed that patients with higher depression scores had more severe dry eye. As a result, we recommend performing ophthalmic examinations in newly diagnosed MDD patients.
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    The Detect Consensus Report on Attention Deficit/Hyperactivity Disorder and Its Management Among Turkish Children And Adolescents (Detect: consensus report on ADHD among Turkish youth)
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-04-07) Ogutlu, Hakan; Kutuk, Ozlem Meryem; Tufan, Ali Evren; Akay, Aynur Pekcanlar; Gundogdu, Ozlem Yildiz; Ercan, Eyup Sabri
    Attention Deficit/Hyperactivity Disorder (ADHD) is one of the most common and heritable neurodevelopmental disorders which may last through the life-span. A consensus report on diagnosis and management of ADHD among Turkish youth was prepared previously. However, the participants as well as the management options were rather limited and developments in the past decade necessitated a revision and update of the consensus. Therefore, this review aims to summarize the consensus among Child and Adolescent Psychiatrists from Turkiye on the nature and management of pediatric ADHD. For those aims, the etiology of ADHD, diagnostic and evaluation process, epidemiology, developmental presentations, differential diagnoses and comorbidities, course/outcome and pharmacological as well as non-pharmacological management options were reviewed and suggestions for clinical practice are presented. Since ADHD is a chronic disorder with wide-ranging effects on functionality that is frequently accompanied by other mental disorders, a multidimensional therapeutic approach is recommended. However, since the disorder has neurobiological basis, pharmacotherapy represents the mainstay of treatment. Additional therapies may include psychosocial therapy, behavioral therapy, school-based therapeutic approaches, and family education. This review provides recommendations for ADHD at the national and global levels. It contains information about ADHD that will contribute to and facilitate clinicians' decision-making processes. It is advisable to consider this guideline in clinical practice.
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    The Capacitance/Conductance And Surface State Intensity Characteristics Of The Al/(Cmat)/P-Si Structures
    (Başkent Üniversitesi Fen Edebiyat Fakültesi, 2024-02-01) Cetinkaya, H. G.; Bengi, S.; Sevgili, O.; Altindal, S.
    To determine the Al/(CMAT)/p-Si structure's admittance analysis, capacitance/conductance versus frequency (C/G-V-f) data was obtained in the 3 kHz-3 MHz and -2/4 V ranges at room temperature. The powder form of CeMgAl11O19: Tb (CMAT) was thermally evaporated onto the front of p-Si wafer at 10-6 Torr as interfacial layer. From the Nicollian and Brews method, voltage-dependent spectra of Rs were derived for various frequencies. The parallel conductance and low-high frequency capacitance (CLF-CHF) techniques, respectively, were used to determine the voltage and frequency dependent spectra of Nss and their lifetime (tau). Surface states (Nss), which are identified by admittance measurements, emerge at the M/S interlayer because of high capacitance and conductance values at low frequencies. This can also be explained by the Nss's ability to track ac signals well at lower frequencies. The normalized parallel conductance versus frequency (Gp/omega-f) plot under various biases shows a peak because of Nss existence. x-ray diffractometer (XRD) was used for structural investigation and the average crystal size (D) of the nanocrystals (CMAT) was found to be less than 0.34 nm by using the Debye-Scherer's equation.
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    A Partial Epithelial-Mesenchymal Transition Signature For Highly Aggressive Colorectal Cancer Cells That Survive Under Nutrient Restriction
    (JOURNAL OF PATHOLOGY, 2024-01-24) Pastorino, Gil A.; Sheraj, Ilir; Oral, Goksu; Gulec Taskiran, Aliye Ezgi; Palmisano, Ralph; Schneider-Stock, Regine
    Partial epithelial-mesenchymal transition (p-EMT) has recently been identified as a hybrid state consisting of cells with both epithelial and mesenchymal characteristics and is associated with the migration, metastasis, and chemoresistance of cancer cells. Here, we describe the induction of p-EMT in starved colorectal cancer (CRC) cells and identify a p-EMT gene signature that can predict prognosis. Functional characterisation of starvation-induced p-EMT in HCT116, DLD1, and HT29 cells showed changes in proliferation, morphology, and drug sensitivity, supported by in vivo studies using the chorioallantoic membrane model. An EMT-specific quantitative polymerase chain reaction (qPCR) array was used to screen for deregulated genes, leading to the establishment of an in silico gene signature that was correlated with poor disease-free survival in CRC patients along with the CRC consensus molecular subtype CMS4. Among the significantly deregulated p-EMT genes, a triple-gene signature consisting of SERPINE1, SOX10, and epidermal growth factor receptor (EGFR) was identified. Starvation-induced p-EMT was characterised by increased migratory potential and chemoresistance, as well as E-cadherin processing and internalisation. Both gene signature and E-cadherin alterations could be reversed by the proteasomal inhibitor MG132. Spatially resolving EGFR expression with high-resolution immunofluorescence imaging identified a proliferation stop in starved CRC cells caused by EGFR internalisation. In conclusion, we have gained insight into a previously undiscovered EMT mechanism that may become relevant when tumour cells are under nutrient stress, as seen in early stages of metastasis. Targeting this process of tumour cell dissemination might help to prevent EMT and overcome drug resistance. (c) 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
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    Mass Migration Governance And Openness Toward Refugees: Comparing Germany And Turkey
    (INTERNATIONAL MIGRATION, 2024-01-22) Erden, Yigit; Ozcurumez, Saime
    This study seeks answers to the question: 'Under what conditions do communities with migration experience in refugee-receiving states become more open toward accepting more refugees and why?'. The research seeks answers to this question by examining the attitudes of individuals (N = 37) from Turkey who have been living in Germany for at least a year and who have sufficient familiarity with the characteristics of governance of mass migration in both countries. The findings suggest that the respondents are more pessimistic about the consequences of Turkey accepting more refugees in the future, while they are optimistic about the outcomes of the arrival of more refugees in Germany. This study posits that receiving communities' perceptions about the host state's regulatory and institutional capacity in managing mass migration and integration of refugees shape their attitudes toward the possibility of the influx of more refugees over time. The findings indicate that efficient mass migration governance in a receiving state is identified through four characteristics: (i) admission of educated refugees, (ii) proper refugee registration procedures, (iii) systematic integration processes, and (iv) effective monitoring and law enforcement mechanisms. The study concludes that local communities view public authorities as the key actors in managing the consequences of mass migration and establishing and sustaining good mass migration governance at the receiving state level is likely to facilitate positive attitudes towards accepting more refugees.