Fen Edebiyat Fakültesi / Faculty of Letters and Science

Permanent URI for this collectionhttps://hdl.handle.net/11727/1396

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End date: 2019Subject: search.filters.subject.GENE

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    Fission Yeast srm1 is Involved in Stress Response and Cell Cycle
    (2017) Gevrekci, Aslihan Ors; 28345120
    Polyamines are well-conserved, multifunctional polycations that contribute to a number of processes in the cells such as cell cycle, apoptosis, stress response, and gene expression. Therefore, polyamine levels should be kept under strict regulation by specific polyamine transporters and polyamine synthases. In this study, the aim is to experimentally characterize a predicted spermidine synthase gene srm1, which was identified upon sequence similarity, in fission yeast Schizosaccharomyces pombe. In an attempt to understand the role of this gene in cell cycle and stress response, deletion mutant of srm1 was generated and analyzed in terms of cell cycle regulation and environmental stress response. The results showed that srm1 Delta cells had elongated cell size and were sensitive to osmotic stress, while they showed no sensitivity to DNA-damaging agents. To the best of our knowledge, this is the first experimental characterization of srm1 gene and its role in cell cycle progression and stress response.
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    FUNCTIONAL CHARACTERIZATION OF SPERMINE FAMILY TRANSPORTER caf5(+) IN Schizosaccharomyces pombe (Lindner)
    (2019) Ors Gevrekci, Aslihan
    Polyamines are well conserved polycationic molecules that are known to interact with nucleic acids and contribute to multiple functions including cell cycle and stress response. The transport of polyamines in and out of the cell is driven by polyamine transporters that play a significant role in polyamine homeostasis. Schizosaccharomyces pombe (Lindner) caf5(+) gene codes for a spermine family transporter that is yet to be characterized functionally. This study aims to understand the contribution of caf5(+) on different processes previously associated with polyamines, by reverse genetics. Deletion mutants of caf5(+), which are viable in normal conditions, were scanned for multiple cellular processes. The results showed that caf5(+) deletion caused shorter cell length and slightly faster growth rate at the optimum conditions. caf5. cells also showed sensitivity to high doses of UV irradiation, while no sensitivity was observed against osmotic stress or another DNA damaging agent hydroxyurea. The mutants could successfully go through different phases of mitosis and meiosis as observed by DNA and septum staining. In summary, caf5(+) gene is involved in normal growth and cell cycle progression, as well as stress response upon UV irradiation.