Tıp Fakültesi / Faculty of Medicine

Permanent URI for this collectionhttps://hdl.handle.net/11727/1403

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    Effects of Intracameral Drugs and Dyes on Corneal Endothelial Cell Apoptosis in a Rat Model: An In Vivo and In Vitro Analysis
    (2022) Bayar, Sezin Akca; Ozturker, Zeynep Kayaarasi; Akova, Yonca Aydin; Bilezikci, Banu; Karabay, Gulten; 0000-0001-5109-755X; 36578186; AAJ-2406-2021
    Objectives: To evaluate the effects of intracameral drugs and dyes on rat corneal endothelial apoptosis and cell morphology. Materials and Methods: The right eyes of 72 rats were injected intracamerally with 1% lidocaine, 0.01% adrenaline, triamcinolone acetonide (TA) 4 mg/mL, 1% trypan blue (TB), 0.5% indocyanine green (ICG), and fortified balanced salt solution as control. Corneal samples were taken 1 day and 1 week post-injection. Corneal endothelial apoptosis was assessed by the TUNEL technique, and the ratio of apoptotic cells in each group was compared with the control. Corneal endothelial cell morphology was evaluated in each specimen by transmission electron microscopy. Results: The mean apoptotic endothelial cell ratio was significantly higher at 1 day and 1 week after intracameral adrenaline injection when compared to controls (p=0.03 and 0.021, respectively). TB caused a significantly higher apoptotic cell ratio when compared to controls at 1 week after injection (p=0.043). Lidocaine caused a higher apoptotic cell ratio compared to TA and ICG at 1 week, although not statistically significant (p=0.058, 0.09, 0.69, respectively). In all experimental specimens, transmission electron microscopy showed morphological changes associated with apoptosis. Conclusion: This study showed that intracameral adrenaline, TB, and lidocaine injections may have toxic effects on corneal tissue, as indicated by ultrastructural and histopathological alterations. Therefore, these agents should be used with caution in intraocular surgery.
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    Editorial: Cell Death and Targeted Cancer Therapies
    (2022) Bagci-Onder, Tugba; Kutuk, Ozgur; Chonghaile, Triona Ni; Knippschild, Uwe; 000829492900001
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    Protective Effect of Spirulina on Cisplatin-Induced Ototoxicity: A Functional and Histopathological Study
    (2022) Tahir, Emel; Buyuklu, Adnan Fuat; Ocal, Fatma Ceyda Akin; Gurgen, Seren Gulsen; Sarsmaz, Hayrunnisa Yesil
    Objective: The purpose of this study was to evaluate the protective effect of an antioxidant and anti-inflammatory agent, "spirulina," against cisplatin-induced ototoxicity in rats. Methods: Twenty-eight adult Sprague-Dawley rats were divided into 4 groups. Before drug administration, distortion product otoacoustic emission and auditory brainstem response tests were performed. Group 1 (n =7) received 1 mg of intraperitoneal saline. Group 2 (n=7) received a single dose of intraperitoneal cisplatin at 15 mg/kg/day. Group 3 (n=7) received oral spirulina at 1000 mg/kg/day for 10 days. Group 4 (n=7) received a single i.p. dose of cisplatin at 15 mg/kg/day, followed by 10 days of oral spirulina at 1000 mg/kg/day. The final distortion product otoacoustic emission and auditory brainstem response measurements were provided 10 days after the initial drug administration. Cochleas were removed, the histochemical examination was performed by caspase-3, caspase-9, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling methods. Results: Initially, there were no significant differences in distortion product otoacoustic emission and auditory brainstem response measurements between groups. Following cisplatin treatment, the mean difference in signal to noise ratio values was lower in the cisplatin + spirulina group compared to the cisplatin-only group. The increase in auditory brainstem response thresholds was more significant in the cisplatin-only group than in the cisplatin + spirulina group. Posttreatment auditory brainstem response latencies were prolonged in cisplatin and cisplatin + spirulina groups; however, a significant difference was obtained between these 2 groups. The cisplatin + spirulina group had a lower density of apoptotic cells than the cisplatin-only group. Conclusion: Spirulina has no adverse effects on cochlear functions and may provide some protection against cisplatin-induced ototoxicity.