Tıp Fakültesi / Faculty of Medicine
Permanent URI for this collectionhttps://hdl.handle.net/11727/1403
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Item Effect of Acrylamide Treatment on Arginase Activities and Nitric Oxide Levels in Rat Liver and Kidney(2014) Ozturan-Ozer, Eda; Ucar, Gulberk; Helvacioglu, Fatma; Akaydin-Aldemir, Derya; Turkoglu, Suna; https://orcid.org/0000-0001-6543-4043; https://orcid.org/0000-0002-6026-0045; https://orcid.org/0000-0003-4805-1918; AAK-3000-2021; AAH-8887-2021; AAJ-2243-2021Aim: To evaluate the effects of acrylamide treatment on the activities of rat liver, kidney arginase activities and nitric oxide levels considering the possible induction of oxidative stress. Materials and methods: Serum aminotransferase activities, blood-urea nitrogen (BUN) and creatinine concentrations and tissue malondialdehyde (MDA), reduced glutathione (GSH), total nitrite concentrations and arginase activities were evaluated in groups. Histopathological analysis was performed. Results: Acrylamide treatment did not modulate liver and kidney serum markers. Hepatic MDA, GSH concentrations did not change whereas they were elevated in kidney tissues of high dose treated group (p<0.05). Arginase activity in grain liver tissue decreased (p<0.0001), but specific activity did not alter. Total nitrite concentrations increased in high dose treated group (p<0.05). In kidney, high dose of acrylamide treatment elevated activity and specific activity of arginase (p<0.05). No alteration was detected in total nitrite levels. Ultrastructural alterations were detected in epithelial cells of proximal tubules in kidney sections of the rats treated with high dose of aculamide. Conclusion: Liver seems to protect itself against acrylamide toxicity whereas, kidney can be considered as a probable target tissue for acrylamide-induced oxidative stress.Item Impact of Rho-Kinase Inhibitor Hydroxyfasudil in Protamine Sulphate Induced Cystitis Rat Bladder(2015) Akin, Yigit; Bozkurt, Aliseydi; Erol, Huseyin S.; Halici, Mesut; Celebi, Fikret; Kapakin, Kubra A. T.; Gulmez, Hakan; Ates, Mutlu; Coban, Abdulkadir; Nuhoglu, Baris; 0000-0001-5467-3743; 26663691; Y-1659-2019ObjectivesThe objective of the present study was to evaluate anti-inflammatory effects of hydroxyfasudil in a protamine sulfate (PS) induced cystitis rat model. Additionally, we investigated prevention of bladder overactivity (BO), and tissue damage in these experiments. MethodsAnimals were divided into four groups. In Groups 1 and 2, chemical induced cystitis model was created by administrating intravesical PS with PE50 catheter by the transurethral route. In Group 1, Rho-kinase inhibitor hydroxyfasudil was administered intaperitoneally, and in Group 2, subjects were administered a corresponding volume of saline in the same way. In Group 3, vehicle was administered intravesically and hydroxyfasudil was administrated intraperitoneally. Group 4 was a control Group, and the vehicle was administered intravesically and intraperitoneally. Micturition frequencies were recorded. Biochemical analyses were performed for oxidative stress, and pathological evaluations were investigated. In vitro contractions of bladder tissue strips were measured in tissue-bath. ResultsThere were significantly lower Lipid peroxidase levels and higher levels of Glutathione in Group 1 than Group 2 (P=0.016, P=0.001, respectively). There was generally more inflammation in Group 2 than the other groups as determined by microscopy. There were significantly higher frequencies of micturition, lower volume, and mean voided maximum urine output after PS administration in Groups 1 and 2. In vitro contraction responses of bladder strips to potassium chloride and acetylcholine were statistically higher in Group 2 than Groups 1 and 3. ConclusionsSignificant reduction of inflammation by affecting the anti-oxidant defense systems was provided by hydroxyfasudil. Decreased in vitro responses to contractions of bladder smooth muscle strips were obtained. Hydroxyfasudil may be a potential new therapeutic option for inflammation and BO, in rat bladder.Item Superoxide Dismutase and Catalase Genotypes in Pediatric Migraine Patients(2015) Saygi, Semra; Erol, Ilknur; Alehan, Fusun; Yalcin, Yaprak Yilmaz; Kubat, Gozde; Atac, Atac, Fatma Belgin; 0000-0002-3530-0463; 0000-0001-6868-2165; 0000-0002-9337-9106; 0000-0002-8522-5078; 25818327; AAK-4825-2021; ABG-9966-2020; ABB-4078-2020; AAB-1203-2021This study compared superoxide dismutase (SOD) and catalase (CAT) alleles in 97 consecutive children and adolescents with migraine to 96 healthy children and adolescents. Isolated genomic DNA was used as a template for SOD1 (35 A/C), SOD2 16 C/T, and CAT2 [(-262 C/T) and (-21 A/T)] allele genotyping. The SOD2 16 C/T genotype and C allele frequency differed significantly between controls and migraine (P = .047; P = .038). CAT -21 AA genotype and A allele frequency were significantly higher in both migraine with aura patients (P = .013; P = .004) and migraine without aura patients (P = .003; P = .001) compared to controls. To our knowledge, this is the first demonstration of differences in SOD and CAT genotypes between pediatric migraine patients and age-matched controls. Further studies on the functional implications of these genetic variants on neural antioxidant capacity and the use of antioxidant modulators for migraine treatment are warranted.Item Comparison of Ischemia Modified Albumin Levels with Total Oxidant, Total Antioxidant Status, Oxidative Stress Index in Carbon Monoxide Poisoning(2014) Durukan, Polat; Koyuncu, Murat; Salt, Omer; Kavalci, Cemil; Ozkan, Seda; Muhtaroglu, Sebahattin; Kavalci, Gulsum; Ozdemir, Caglar; Duzgun, Ali; Ikizceli, Ibrahim; https://orcid.org/0000-0003-2529-2946; AGG-1308-2022Aim: The most common cause of death in CO poisoning is ventricular arrhythmias due to tissue hypoxia. In this study we aimed to investigate the relationship between severity of poisoning and Total Oxidant Status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) and also change in the levels of ischemia modified albumin (IMA) and neutrophil gelatinase-associated lipocalin (NGAL) over time in the patients with CO poisoning. Material and methods: This study was performed at Erciyes University Faculty of Medicine, Department of Emergency Medicine. Fifty patients between the ages of 18-65 who were diagnosed CO poisoning in the emergency department were included in the study. As a control group 30 adult individuals with no history of any disease were included in the study. Ischemia modified album, NGAL, OSI, TOS and TAS levels were studied. Mann-Whitney U test was using to compare of control and patient group. The Wilcoxon test was used to compare the change in TAS, TOS, OSI, IMA, NGAL, COHb and lactate. p<0.05 was considered as statistically significant. Results: When the 0th hour levels of Lactate, TOS, OSI, and IMA and TAS of the patient group were compared to the control group, there was a significant difference between these groups (p <0.05). There was no significant difference in terms of the NGAL level (p> 0.05). When 0th, 3rd, 6th, 12 and 24th hrs TAS, TOS, OSI, IMA, NGAL and lactate levels compared with each other, there was no difference between them (p>0.05). Conclusion: The levels of IMA, TOS, TAS and OSI were detected high in CO poisoning, but it is not meaningful in evaluating the effectiveness of treatment.Item Obesity and Oxidative Stress in Patients with Different Periodontal Status: A Case-Control Study(2017) Atabay, V. E.; Lutfioglu, M.; Avci, B.; Sakallioglu, E. E.; Aydogdu, A.; https://orcid.org/0000-0002-1738-371X; 26932579; ABA-1100-2020Background and ObjectiveObesity has become an important global health concern as obesity-associated adiposity is supposedly related to systemic immunologic and inflammatory alterations. The aim of this study was to evaluate the effects of obesity on periodontally healthy and diseased tissue according to the changes in malondialdehyde (MDA), protein carbonyl (PC) and total antioxidant capacity (TAOC) levels in gingival crevicular fluid as biomarkers of oxidative stress (OS). Material and MethodsThe study sample comprised systemically healthy normal-weight (n = 45) and obese (n = 48) adults. Obesity was diagnosed according to body mass index, waist circumference and waist/hip ratio. Periodontal status was evaluated according to plaque index, gingival index, bleeding on probing, probing depth and clinical attachment level. Participants were distributed among six groups according to obesity and periodontal status, as follows: normal weight+periodontally healthy (NH); normal weight+gingivitis (NG); normal weight+generalized chronic periodontitis (NCP); obese+periodontally healthy (OH); obese+gingivitis (OG); and obese+generalized chronic periodontitis (OCP). MDA, PC and TAOC levels were measured using ELISA. ResultsThe MDA and PC levels in gingival crevicular fluid varied among groups, as follows: NCP > NG > NH (p < 0.01) and OCP > OG > OH (p < 0.01). Conversely, the levels of TAOC in gingival crevicular fluid varied as follows: NCP < NG < NH (p < 0.01) and OCP < OG < OH (p < 0.01). Paired comparisons conducted according to periodontal status showed MDA and PC levels to be higher, and TAOC levels to be lower, in the OCP group than in the NCP group, in the OG group than in the NG group and in the OH group than in the NH group. However, only the differences between the OCP and NCP groups were significant (p < 0.01). In both obese and normal-weight individuals, clinical assessments showed significant, positive correlations with MDA and PC levels and negative correlations with TAOC levels (p < 0.01). ConclusionObesity may influence periodontal tissue destruction and disease severity by increasing the level of oxidative stress in the presence of periodontal disease.