Tıp Fakültesi / Faculty of Medicine
Permanent URI for this collectionhttps://hdl.handle.net/11727/1403
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Item Dexamethasone Effects on Vascular Flow and Organ Injury in Septic Mice(2014) Aytac, Huseyin Ozgur; Iskit, Alper B.; Sayek, Iskender; https://orcid.org/0000-0002-3583-9282; 24582065; AAJ-7913-2021Background: To demonstrate the effects of low-dose dexamethasone treatment on mesenteric artery blood flow, oxidative injury, vascular reactivity, and survival in Swiss albino mice with intra-abdominal polymicrobial sepsis accomplished by cecal ligation and puncture (CLP). Methods: Mice were allocated to CLP + saline, CLP + dexamethasone, sham + saline, and sham + dexamethasone subgroups to evaluate blood flow, organ injury, and vascular response to consecutive phenylephrine administrations at 24, 48, and 72 h. Survival rates were also evaluated in a different group of mice. Dexamethasone (1 mg/kg/d) and saline (4 mL/kg/d) were administered intraperitoneally to mice 2 h after CLP or sham procedure, whichever appropriate, and repeated once a day until evaluation time at 48 and 72 h. Relaparotomy was performed at the concerned time and mesenteric blood flow was measured, and liver, lung, and peritoneum samples were obtained. Alteration in mesenteric blood flow response to intravenous phenylephrine injections was recorded at the related time intervals in different mice groups. Survival group was followed up by 7-d administration of dexamethasone or saline for 18 d. Results: The significant fall in mesenteric blood flow after CLP ameliorated with dexamethasone treatment at 48 and 72 h. Dexamethasone also diminished the malonyl dialdehyde level, which is an indicator of organ injury raised after CLP, at 24 h in liver, lung, and peritoneum samples. Dexamethasone therapy has significantly enhanced the vascular response to phenylephrine injections at all doses; however, no change was observed in survival rates. Conclusions: Low-dose dexamethasone has beneficial effects on mesenteric blood flow and organ injury in experimental sepsis models. (C) 2014 Elsevier Inc. All rights reserved.Item The Protective Effect of Adrenocorticotropic Hormone Treatment Against Noise-Induced Hearing Loss(2018) Mutlu, Ahmet; Ocal, Fatma Ceyda Akin; Erbek, Seyra; Ozluoglu, Levent; 0000-0001-9022-921X; 0000-0002-8453-6069; 0000-0002-2150-0237; 29747961; AAI-2097-2019; AAJ-2445-2021; AAI-8020-2021Objective: NIHL is a common problem, and steroids are the most effective treatment option. In this study, we aimed to evaluate the protective effects of the synthetic adrenocorticotropic hormone (ACTH) analogues, which induce endogenous steroid secretion, against noise-induced hearing loss (NIHL) and to compare their effectiveness with that of steroid treatment. Methods: Twenty-four male Sprague Dawley albino rats were divided into four subgroups as follows: group 1 (n = 6) control, group 2 (n = 6) saline, group 3 (n = 6) dexamethasone (2 mg/kg/ day intramuscularly [IM]), group 4 (n = 6) ACTH analogue (0,4 mg/kg/day IM), respectively. Three groups (groups 2-4) were exposed to white noise (105 dB SPL, 12 h). All the rats were evaluated for hearing thresholds of 10 kHz, 20 kHz, and 32 kHz via acoustic brainstem responses (ABR) measurement. After the basal threshold measurements, measurements were repeated immediately after the noise and on day 7 and day 21. Results: Both steroid and ACTH analogue groups showed significantly better hearing outcomes than the saline group on day 7 (p < 0.001) and day 21 (p < 0.001) after the noise exposure. No superior treatment effect was demonstrated in either the steroid or ACTH analogue group. None of the related intervention groups reached the basal hearing thresholds. Conclusion: Steroids were effective drugs for the treatment of NIHL. ACTH analogues also demonstrated promising therapeutic effects for NIHL. Further studies to establish ACTH analogues as an alternative NIHL treatment option to steroids are needed. (C) 2018 Elsevier B.V. All rights reserved.