Tıp Fakültesi / Faculty of Medicine

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Author: search.filters.author.Demirbilek, MugeHas files: No

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    In Vitro Evaluation of E. coli And S. epidermidis Adhesion on Dressing Materials Used in Hypospadias Repair
    (2014) Soyer, Tutku; Bostanoglu, Ebru; Aslan, Mustafa Kemal; Boybeyi, Ozlem; Demirbilek, Muge; Gocmen, J. Sedef; JWP-3001-2024
    Background An in vitro study was performed to evaluate the adherence of Escherichia coli and Staphylococcus epidermidis to different dressing materials. Methods Hydrophyl gauze swab (GS), sterile flax swab (FS), and their nitrofurazone (Furacin (R)) absorbed forms (GSF and FSF), sterile paraffin-covered gauze with chlorhexidine acetate (0.5 %) (Bactigrass (R), BG), and semipermeable transparent film gauze (Tegaderm (R), TG) were used in the study. A 1 cm(2) of each material was placed in culture plates, and E. coli ATCC 25922 and S. epidermidis ATCC 12228 were suspended in each plate. After 24, 48 h, and 1 week of incubation period, colony counts were calculated in colony-forming units (CFU) per square centimeter unit. Bacterial adhesion was compared between groups, and each dressing material was also compared for different incubation periods (24, 48 h, and 1 week). Results The colony counts were significantly decreased in GSF and FSF groups (Kruskal-Wallis test, p < 0.05). There was no difference for E. coli adhesion in GS, FS, BG, and TG groups (p > 0.05). The significant difference detected for S. epidermidis adhesion in the first 24 to 48 h could not be detected at the end of a 1-week period. There was no statistical difference in groups when compared for 24-, 48-h, and 1-week periods of incubations (Wilcoxon signed-rank test, p > 0.05). In BG group, E. coli adhesion was increased, and S. epidermidis adhesion was decreased without a significant difference at the end of a 1-week period (p > 0.05). Conclusions Nitrofurazone absorbed sterile gauze materials have less bacterial adhesion. In vitro adhesion of E. coli and S. epidermidis did not differ between the 24-h and 1-week period. E. coli adhesion to BG can be increased if the duration of dressing prolonged up to a 1-week period. Level of Evidence: Not ratable.
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    Efficacy of Multipurpose Contact Lens Solutions Against ESBL-Positive Escherichia coli, MRSA, and Candida albicans Clinical Isolates
    (2014) Demirbilek, Muge; Evren, Ebru; 24756120; JWP-3001-2024
    Objectives: The antimicrobial effects of multipurpose contact lens solutions (MPSs) have been evaluated according to ISO 14729 standards. The aim of this study was to assess the efficacy of commercially available MPSs against extended-spectrum beta lactamases (ESBL)-producing Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), and Candida albicans clinical isolates. Methods: Three commercially available contact lens solutions (Opti-Free Expresss, ReNu MultiPlus, and Solo Care Aqua) were tested against 18 ESBL-positive E. coli clinical strains, 20 MRSA clinical strains, and 20 C. albicans clinical strains. The stand-alone assays for bacteria and fungi were performed according to ISO 14729 criteria, and all samples were evaluated after 2, 4, and 24 hours of incubation. The numbers of viable microorganisms were evaluated by the plate-counting method. Results: All MPSs demonstrated at least 3 log reduction in colony-forming units (CFU) for all bacterial isolates and 1 log reduction in CFU for all yeast isolates, which meets ISO 14729 standards. Although no statistically significant differences were obtained among MPSs for bacterial isolates, variable responses were observed against clinical isolates: 5% povidone-iodine was more effective compared with Solo Care Aqua for C. albicans clinical strains (P<0.05); and all solutions were effective after an incubation time of only 2 hrs. The MPSs showed good activity against S. aureus, E. coli, and C. albicans. Conclusions: Although effective log reductions were provided with all MPSs, the reduction was variable depending on the strains tested. Multipurpose contact lens solutions should be tested under ISO 14729 standards for both standard and clinical strains.