Diş Hekimliği Fakültesi / Faculty of Dentistry
Permanent URI for this collectionhttps://hdl.handle.net/11727/2120
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Item Effect of Chitosan and EDTA Solutions on Bond Strength of Two Different Calcium Silicate Based Materials(2023) Koc, Cemre; Gulsahi, Kamran; Kahya, Naime DidemObjective: The aim of the present study was to evaluate the potential of using chitosan-based chelating agents to improve the bond strength of mineral trioxide aggregate (MTA) Angelus (Londrina, PR, Brazil) or MTA Repair high plasticity (Londrina, PR, Brazil). Materials and Methods: A total of 60 dentine discs were obtained from 15 freshly extracted human maxillary central incisors. Two canal -like holes were drilled and the disks were divided into four groups, as following; group 1: chitosan solution with acetic acid; group 2: chitosan solution with lactic acid; group 3: 17% ethylenediaminetetraacetic acid; and group 4: distilled water. Discs were subjected to the tested chelating solution for smear layer removal for 3 min. The two holes of the same dentine discs were each randomly filled with one of the tested materials. The push -out test was performed and data were analyzed using 2 -way analysis of variance test with a 5% significance level. Results: No significant differences were observed for the type of tested material (p=0.153) and the interaction between tested material and solution (p=0.922); however, there was a significant difference among chelating agents (p=0.001). Conclusion: Both materials showed similar bond strength regardless of the a chelating agent was used or not. All chelating agents significantly decreased the push -out strength of both materials, except for the chitosan solution prepared with acetic acid.Item The Effect of Temperature and Contact Time of Sodium Hypochlorite on Human Roots Infected with Enterococcus Faecalis and Candida Albicans(2014) Gulsahi, Kamran; Tirali, R. Ebru; Cehreli, S. Burcak; Karahan, Zeynep Ceren; Uzunoglu, Emel; Sabuncuoglu, Bizden; https://orcid.org/0000-0003-3510-7265; https://orcid.org/0000-0001-6487-3984; 23011474; AAX-5565-2021; AAD-2907-2020; AAD-6138-2021The aim of this study was to evaluate the effectiveness of 2.5 % NaOCl at different temperature and time intervals on Enterococcus faecalis and Candida albicans-infected human roots. A total of 112 root cylinders prepared from extracted single-rooted humans were infected by E. faecalis (Group A, n = 56) or C. albicans (Group B, n = 56); 3 root cylinders served as negative controls. Both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with NaOCl at two different temperatures (25 or 37 A degrees C). Microorganism growth was controlled at the 24th and 48th hours. Statistical analysis was performed using the Chi-square test. While NaOCl at 25 A degrees C for 5 min was the most effective irrigation regimen to eliminate E. faecalis (p < 0.001), NaOCl at 37 A degrees C for 5 min exhibited significantly superior antifungal properties (p < 0.05). At the same contact times, difference in the temperature of NaOCl did not affect the growth of either E. faecalis or C. albicans. As a result, the irrigation time of NaOCl was more effective than the temperature to eliminate E. faecalis, while pre-heating of NaOCl to 37 A degrees C increased its effectiveness on C. albicans at 5 min contact time.Item Effect of Adhesive Systems on Microleakage in Endodontically Treated Teeth Restored with Glass Fiber Posts(2014) Ozdemir, Erdem; Gulsahi, Kamran; Korkmaz, Yonca; Erkut, Selim; Tuncer, Abidin Hakan; https://orcid.org/0000-0003-3510-7265; AAX-5565-2021The effect of adhesive systems on microleakage in endodontically treated teeth restored with glass fiber posts was studied. Forty carious and crack-free premolars were prepared using a stepback technique and obturated with gutta-percha. The endodontically treated roots were randomly divided into four groups (n = 10). Glass fiber posts cemented following adhesive luting agents; group 1: All Bond 3/Duo Link (ALB/DL), group 2: One-Step Plus/Duo-Link (OSP/DL), group 3: Panavia F 2.0/Panavia ED Primer II (PAN/ED), and group 4: Rely X Unicem (RU). All specimens were thermal cycled and stored in basic fuchsin solution for one week. The roots were sectioned horizontally into three sections: apical, middle, and coronal. The occlusal direction of each section was digitally photographed under a stereomicroscope. Dye penetration area was calculated as the basic fuchsin infiltrated surface divided by total dentin. The data were statistically analyzed using the Mann-Whitney U and Wilcoxon Signed Ranks Tests (alpha = 0.05). The mean area measurements were ranged between 0 and 3.7357. According to the Mann-Whitney U test, there was no significant difference among the groups and sections (p > 0.05). Intragroup comparisons showed a significant difference between coronal-apical and middle-coronal sections in Panavia group (p = 0.043) by Wilcoxon Signed Test. It was found that there was no significant difference among four groups and the apical, coronal, and middle sections. The only difference was found for the group of PAN/ED between coronal-apical and coronal-middle sections.Item Assessment of Endodontic Emergency Care in a COVID-19 Pandemic(2022) Sisli, Selen Nihal; Koc, Cemre; Sayin, Ceren Efe; Gulsahi, Kamran; https://orcid.org/0000-0003-3510-7265Objective: This study compared the aerosol-generating and non-aerosol -generating endodontic emergency procedures' success and assessed the outcome of endodontic treatments initiated before a pandemic but could not be completed in the targeted time. Materials and Methods: Emergency treatments were performed according to symptoms of teeth. Treatment success or failure was determined according to patients whether not re-referral with untimely pain. Short-term outcome and complications arising from teeth, which endodontic treatments were prolonged were also recorded. A chi-square test was used in the statistical analysis, and p<0.05 was considered significant. Results: The aerosol-generating procedure group's success rate was 86.2%, while it was 70.0% in the non-aerosol generating procedure group (p=0.050). The short-term survival rate of teeth was 83.7% in patients whose endodontic treatment had been prolonged. Conclusion: Considering the pros and cons, each emergency patient should be evaluated case-by-case.Item Pre-eruptive Intracoronal Resorption of Permanent Dentition: A New Classification and a Multidisciplinary Study(2023) Yuksel, Halil Tolga; Turkmenoglu, Aysegul; Celikkol, Berk; Evirgen, Sehrazat; Gulsahi, Kamran; Gulsahi, Ayse; https://orcid.org/0000-0003-3510-7265; 36317538The aims of this study were to develop a new classification for pre-eruptive intracoronal resorption(PIR) with different resorption areas and sizes and to compare the new classification scores among observers from different specialities and professional experience. The PIR was evaluated according to the new classification by two dentomaxillofacial radiologists and two endodontists. Information regarding the patient's age, gender, systemic diseases, the number of PIR, the affected tooth area and the size of the PIR was recorded. The new classification system showed that PIR six and seven defects were the most detected. Regarding jaw regions, the highest reliability was seen in the maxillary central teeth between observers I and IV, and maxillary premolar-molar teeth between observers II and III. This study revealed that PIR can be found in both root and crown dentine. Professional experience and specialty and awareness of the PIR defect may affect the diagnosis.Item Comparison of Fracture Resistance of Endodontically Treated Teeth Restored with FiberSite Postsystem and Glass Fiber, Combined with Different Root Canal Sealers(2021) Eren, Birgul; Ozasir, Tufan; Kandemir, Baris; Gulsahi, Kamran; https://orcid.org/0000-0001-6424-2250; https://orcid.org/0000-0001-8778-9005; https://orcid.org/0000-0003-3510-7265; 34725637This study is aimed at evaluating the effects of FiberSite and glass fiber postsystems on the fracture resistance of endodontically restored teeth, when combined with various root canal sealers. Forty human mandibular premolar teeth, each having a single root with anatomically comparable dimension and length, were selected. The teeth in each experimental group (n = 10) were instrumented, obturated, and restored with the following procedures: in group 1, AH Plus root canal sealer and a glass fiber post with a composite core; in group 2, AH Plus root canal sealer and a FiberSite postsystem; in group 3, Sure-Seal Root canal sealer and a glass fiber post with a composite core; and in group 4, Sure-Seal Root canal sealer and a FiberSite postsystem. The specimens were placed on a universal test machine. The fracture resistance of all specimens was tested using generic test equipment, and the value of the force in newton (N) during fracture was measured for each root. A statistical analysis was conducted through the Kruskal-Wallis test as well as the post hoc test. The tests showed a significant difference between groups 2 and 3, with group 3 producing load values that were significantly higher than group 2. In contrast, no significant differences were found to exist between the other groups. Regardless of postsystems, groups 3 and 4 showed higher mean fracture values (but no statistically significant differences) than groups 1 and 2.Item Change in Periapical Lesion and Adjacent Mucosal Thickening Dimensions One Year after Endodontic Treatment: Volumetric Cone-beam Computed Tomography Assessment(2017) Kamburoglu, Kivanc; Yilmaz, Funda; Gulsahi, Kamran; Gulen, Orhan; Gulsahi, Ayse; https://orcid.org/0000-0003-3510-7265; 28132708; AAX-5565-2021Introduction: Changes in periapical lesion dimensions along with mucosal thickening after endodontic treatment have not been studied yet. Therefo1e, the objectives of this study were (1) to obtain linear and volumetric measurements of lesion dimensions in maxillary first molars with periapical pathology and (2) to measure maxillary sinus mucosal thickening in the vicinity of periapical lesions before and 1 year after endodontic treatment by using cone-beam computed tomography (CBCT). Methods: Twenty-one maxillary first molar teeth of 21 patients (14 female and 7 male) with periapical lesion that had local mucosal thickening in the vicinity of the periapical lesion were endodontically treated. A total of 21 maxillary first molar roots (8 mesiobuccal roots, 6 distobuccal roots, and 7 palatal roots), each one from different patients, was included. Pretreatment and 1-year post-treatment CBCT images of each tooth were obtained by using Kodak CS 9300 3D CBCT unit. Width, height, surface area, and volume measurements of periapical lesions and mucosal thickening of the maxillary sinus mucosa in the vicinity of the periapical lesion were measured before and 1 year after endodontic treatment. General linear model (analysis of variance) was used for the comparisons between measurements, and significance was set at P <.05. Regression analysis was also used to test the correlation between different measurements. Results: We found statistically significant differences between mean pretreatment and mean post-treatment measurements conducted by using CBCT images (width, P =.002; height, P <.001; maximum mucosal thickening, P <.001; medium mucosal thickening, P <.001; minimum mucosal thickening, P <.001; surface area, P =.032; and volume, P =.034). Considering gender, age, and root type variables, no significant differences were found for all the measurements conducted (P >.05). There were 36%, 41%, 53%, 54%, 53%, 73%, and 75% mean reductions in lesion width, lesion height, maximum sinus mucosal thickness, medium sinus mucosal thickness, minimum sinus mucosal thickness, lesion surface area, and lesion volume, respectively, before and 1 year after endodontic treatment. Regression analysis of pretreatment lesion volume versus percentage of post-treatment lesion volume change revealed a low regression coefficient (R2 = 16.7%, P >.05), showing a weak linear relationship. Conclusions: CBCT assessment of changes in periapical lesion and mucosal thickening dimensions may reveal useful information regarding endodontic treatment success.Item Resistance to Fracture of Dental Roots Obturated with Different Materials(2015) Celikten, Berkan; Uzuntas, Ceren Feriha; Gulsahi, Kamran; 25756048The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n = 15 root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.Item A Comparison of Er:YAG Laser with Photon-Initiated Photoacoustic Streaming, Nd:YAG Laser, and Conventional Irrigation on the Eradication of Root Dentinal Tubule Infection by Enterococcus faecalis Biofilms: A Scanning Electron Microscopy Study(2017) Gulsahi, Kamran; Ungor, Mete; Ozkaya, Burcu Ozses; Gocmen, Julide Sedef; 0000-0003-3510-7265; 29279728; ABG-7526-2020; AAF-7291-2021; AAX-5565-2021This study evaluated the antimicrobial efficacy of Er:YAG laser activation with photon-initiated photoacoustic streaming (PIPS), Nd:YAG laser disinfection, and conventional irrigation on Enterococcus faecalis biofilms using scanning electron microscopy (SEM). Biofilms were grown on 110 root halves and divided into the following: Groups 1 and 2 (saline and 1% NaOCl with apical position of PIPS, resp.), Groups 3 and 4 (saline and 1% NaOCl with coronal position of PIPS, resp.), Groups 5 and 6 (Nd: YAG laser after saline and 1% NaOCl irrigation, resp.) and Groups 7, 8, and 9 (conventional irrigation with 1% NaOCl, 6% NaOCl, and saline, resp.). SEM images of the apical, middle, and coronal levels were examined using a scoring system. Score differences between Groups 1 and 2 were insignificant at all levels in the remaining biofilm. Group 4 had significantly greater bacterial elimination than Group 3 at all levels. Differences in Nd: YAG laser irradiation between Groups 5 and 6 were insignificant. Groups 7 and 8 were insignificantly different, except at the middle level. Saline group had a higher percentage of biofilms than the others. In this study, PIPS activation with NaOCl eliminates more E. faecalis biofilms in all root canals regardless of the position of the fiber tip.