Diş Hekimliği Fakültesi / Faculty of Dentistry

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Dişeti ve dental folikül dokularında mezenkimal kök hücre araştırması ve plastisitelerinin karşılaştırılması
(Başkent Üniversitesi Diş Hekimliği Fakültesi, 2008) Eroğlu, Tamer; Uçkan, Sinan
Mezenkimal kök hücreler, rejeneratif potansiyelleri, immünsupresif özelikleri ve destek doku olusturma potansiyelleri nedeniyle hücresel tedavi için ilgi çekmektedir. Kemik iliği, göbek kordonu, çevre kanı, amniotik sıvı, periost, yağ dokusu, sinovial membran ve kas gibi birçok kaynaktan elde edilebildiği gibi maksillofasiyal bölgede de MKH izolasyonu ile ilgili çalısmalar bulunmaktadır. Ağız ortamında en kolay ulasılan bölgelerden biri olan ve sürekli yenilenmekte olan disetinin MKH’ler için potansiyel bir kaynak olabileceğiyle ilgili bir çalısmaya literatürde rastlanmamıstır. Ayrıca dental folikül dokusundan MSC izolasyonu ile ilgili sınırlı sayıda çalısma mevcuttur. Çalısmada, çekim endikasyonu konulmus gömülü 20 yas disi olan 6 hastada insizyon esnasında çıkarılan disetinden ve dental folikül dokusundan alınan doku örneklerinden hücre kültürü yapılmıs ve tüm örneklerden adezyon özelliği gösteren MKH’lerin izolasyonu yapılmıs ve kültürde çoğaltılmıstır. Akım sitometri yöntemi ile hücrelerin immünfenotipleri tanımlanmıs ve adiposit, osteosit, kondrosit ve nöronal hücrelere farklılasabilme potansiyelleri arastırılmıstır. Her iki dokudan da gelistirilen MKH’lerin CD105, CD 73, CD 90 gibi stromal antijenleri yüksek oranda (%60-98) tasıdığı gösterilmistir. Hücreler kültürde 8. pasaja kadar ilerletilmis ve analizler 2, 5 ve 8. pasajlarda yapılmıstır. Pasajlar arasında yüzey antijen ekspresyonları yönünden farklılık saptanmamıstır. Kültürde çesitli uyaranlar kullanılarak yapılan farklılasma deneylerinde diseti ve folikülden elde edilen hücrelerin adipojenik ve osteojenik farklılasma kapasitelerinin bulunduğu gösterilmis, kondrojenik farklılasma elde edilmemistir. Ayrıca, uygun uyaranlar ile nöronal hücre morfolojisine değisme olduğu gözlenmistir. Bu çalısmalarda diseti ve dental folikül dokularından elde edilen MKH lere ait farklılık gözlenmemistir. Fibroblastlar ile birçok ortak özellik tasıyan MKH’lerin fibroblasttan ayrımı için, kültürde hücre yoğunluğunun, adezyon özelliklerinin, yüzey antijen ekspresyonlarının ve farklılasma özelliklerinin detaylı karsılastırması yapılmıstır. Baslangıç hücre yoğunluğu, erken veya geç adezyon göstermeleri ve yüzey antijenleri yönünden fibroblast ve MKH’lerin ayırt edilmesine katkıda bulunacak bir farklılık saptanmamıstır. Hücrelerin kök/projenitör özelliğini göstermesi açısından en önemli özellik olan çok yönlü farklılasma kapasiteleri test edildiğinde (8. pasaja kadar), ileri pasajlarda da farklılasma kapasitesinin korunmasının hücrelerin kök/projenitör özellikleri ile uyumlu olduğu, böylelikle genellikle 3.pasajdan sonra farklılasma özelliği bulunmayan fibroblastlardan ayırt edilebileceği düsünüldü. Elde edilen sonuçlar, diseti dokusunun noninvaziv metodla elde edilebilmesi ve defekt bölgesine yakın olması ile bilinen ağız içi kaynaklara ek bir MKH kaynağı olabileceğini düsündürmüstür. Mesenchymal stem cells draw attention for cell based therapy with their regenerative, and tissue supporting potential and immunosuppresive caharacteristics. MSCs can be isolated from several tissues such as bone marrow, umbilical cord, peripheral blood, amniotic fluid, periosteum, fat, synovial membrane and muscle. Like in different tissues, suitable MSC resources at the maxillofacial region have been investigated and related reports are published in the literature. No studies have been published in the literature about the gingival tissue which is the most easiest approachable site in the oral cavity with a high regeneration potential. Furthermore, isolation of MSCs from dental follicle tissues have been described in a few number of studies. In this study, impacted third molars of 6 patients were extracted in a routine surgical procedure and gingival/ dental follicle tissue samples was obtained during the incision and extraction period. Along all samples, MSCs with an adhesion property have been isolated and expanded in culture. Immunophenotyping was perfomed by flow cytometry and adipocyte, osteocyte, chondrocyte, and neuronal differentiation potential of these stem cells were tested and the characteristics of gingival and follicle stem cells were compared. It has been demonstrated that MSCs obtained from both tissues have a high frequency of specific stromal antigens (%60-98) such as CD105, CD 73, CD 90. Cells have been expanded through passage 8 and characterized at 2. 5. 8. passages. No differance have been determined about surface antigen expression speciality between the passages. Differantiation assays with various stimulants in the culture points out that dental follicle and gingiva derived cells have adipogenic and osteogenic differantiation capacity however there was no evidence of chondrogenic differantiation. Furthermore, with suitable stimulants alteration to neuronal cell morphology was observed. In this study there was no difference between the differantiation potentials of MSCs derived from gingival and dental follicle tissue. Because of their similarities, the detailed comparison of plating density, adhesion properties, surface antigen expressions and differantiation potentials have been performed to distinguish MSCs from fibroblasts. Initial plating density, early or late adhesion properties and surface antigen expressions did not contribute to distinguish MSCs from fibroblasts. Multilineage differantiation potential through the late passages is one of the most important criteria to distinguish MSCs from fibroblasts. The differantiation potential of gingiva and dental follicle derived cells through passage 8 denoted the MSC/progenitor specialities of these cells. Besides the differantiation potential of fibroblasts usually ends at the passage 3. In this study MSCs continuing differantiation through passage 8 was the most important criteria to separate these cells from fibroblasts which has a differantiation potential through passage 3. Obtained results suggest that the gingival tissue is considered as an alternative source of stem cells to the other intraoral stem cell sources with it is in close proximity to the recipient site and can be obtained easily by a non- invasiv method.
Alt çene tam ve kısmi dişsizlik vakalarında diş, implant ve diş-implant destekli hareketli protez uygulamalarında protetik yapılar ve çevre dokulardaki stres, gerinim ve yer değiştirmenin 3 boyutlu sonlu elemanlar stres analiz yöntemi ile değerlendirilmesi
(Başkent Üniversitesi Diş Hekimliği Fakültesi, 2012) Dağlık, Deniz; Çağlar, Alper
Bu çalışmanın amacı; mandibular diş, implant ve diş-implant destekli hareketli protezlerde, kortikal ve trabeküler kemiğin, protezin, dentinin, implantın, periodontal ligament ve tutucu elemanların biyomekanik davranışını, üç boyutlu sonlu elemanlar stres analizi ile değerlendirmektir. Üç boyutlu dişsiz mandibula modelinde dört farklı tasarım oluşturulmuştur. 1. tasarımda, kanin dişler bölgesinde iki adet implant, 2. tasarımda iki kanin diş, 3. tasarımda iki kanin diş ve ikinci molarlar bölgesinde iki adet implant ve 4. tasarımda kanin ve ikinci molar dişler bölgesinde ikişer adet implant locator tutucularla birlikte alt çene hareketli protez desteği olarak kullanılmıştır. Yükleme, sol kanin, premolar ve molar dişlerin cusp tepelerinden vertikal olarak toplam 100 N olarak uygulanmıştır. Sonlu elemanlar stres analizinde 3. ve 4. tasarımlar, 1. ve 2. tasarımlarla kıyaslandığında daha az yer değiştirme ve mukozada daha düşük temas basıncı ve basma stresleri göstermiştir. 4. tasarımda implant çevresi kortikal kemikte, locatorda, locator matrisinde ve housing parçada yükleme yapılmayan taraf posterior implantında en yüksek stresler oluşmuştur. 3. tasarımda posterior bölgeye implant yerleştirilmesiyle kanin dişlerde dentin ve periodontal ligamentte oluşan stresler azalmıştır. Posterior bölgeye implant yerleştirilmesiyle, ikinci molar bölgesinde trabeküler kemikte oluşan gerinim değerleri artmıştır. Posterior implant çevresindeki kortikal kemikte oluşan en yüksek değerler, kemikte rezorbsiyona neden olabileceği belirtilen en yüksek değerlerin altında görülmüştür. Dişsiz alveoler krette oluşan gerinim değerleri Frost’un kemik yapımı ve rezorpsiyonunu tanımladığı stimulasyon pencere aralığı değerlerinin alt sınırına yakın olduğu, posteriora implant yerleştirilmesiyle bu bölge kemiğinde oluşan gerinimlerin arttığı ve stimulasyon pencere aralığı değerleri içinde oluştuğu görülmüştür. En yüksek stresler dört implant kullanılan tasarımda posterior implantta, locator parçada, locator matrisinde ve housing parçada oluşmuştur. Elde edilen stres değerleri materyallerin dayanma sınırının altında oluşmuştur. Bu streslerin ve gerinimlerin, kemik rezorbsiyonu ve locator tutucu parçaya olan etkilerinin uzun dönemli klinik çalışmalarla değerlendirilmesi gerekmektedir. The aim of this study is to evaluate the biomechanical behavior of trabecular and cortical bone, denture, dentin, implant, periodontal ligament, and retentive elements on tooth, implant and implant-tooth supported removable overdenture prosthesis by three dimensional finite element analysis. Four different designs were constructed in 3 dimensional edentulous mandibular model. In the first design, two implants on canine tooth region; in the second design, two canine teeth; in the third design, two canine teeth and two implants on second molar region and in the fourth design, two implants each with locator retentive on canine and second molar region were used as mandibular removable overdenture prosthesis support. Loading was applied vertically on left canine, premolar and molar teeth with a total of 100 N. Designs 3 and 4 showed lower displacement and low contact pressure and compressive stresses at mucosa when compared with model 1 and 2 on finite element analysis. Cortical bone around posterior implants, locator abutment, locator matris and housing at unloaded posterior implant sites showed higher stresses in design 4. Stress values of periodontal ligament and dentin was decreased with the placement of implant at posterior region in model 3. With the placement of implant at posterior region, the strain values were increased on trabecular bone in second molar region. The highest stresses that occur on cortical bone around the posterior implant were lower than the highest values indicated to cause bone resorption. The results showed that strain values occurred on edentulous posterior alveoler ridge were close to the lowest values of numerical analyses of stimulus window scale defined for bone apposition and resorption by Frost; with placement of implant to posterior region strain values were increased in this bone region and all strain values were grouped in Frost’s stimulus window scale. The highest stress values were obtained on posterior implant, locator, matris and housing in design where four implants were used. The stress values were below materials endurance limit. However, effects of obtained stress and strain values on bone resorption and locator retention should be evaluated with long term clinical studies.
Short-Term Evaluation of Nasal Changes After Maxillary Surgery
(2014) Yilmaz, Alev; Polat-Ozsoy, Omur; Arman-Ozcirpici, Ayca; Uckan, Sina
Objective: To determine the nasal soft-tissue profile changes in skeletal Class III patients who underwent maxillary or bimaxillary orthognathic surgery. Materials and Method: This clinical retrospective study consists of 40 patients (19 male and 21 female) who had undergone orthognathic surgery. All patients received single jaw maxillary (9 patients) or bimaxillary surgery (31 patients). Standardized lateral cephalograms obtained before operation and at least 6 months after the operation were used. Twelve measurements (4 skeletal and 8 soft-tissue measurements) were made. The distributions of the variables were checked by Shapiro-Wilk test. Paired-samples t test was used for parametric data and Wilcoxon sign rank test for nonparametric data, to analyze the differences between pre- and postoperative measurements. Results: After orthognathic surgery, SNA and ANB increased; SNB decreased significantly. A significant reduction in nasofrontal angle, TH-Prn, and nasal tip projection and a significant increase in nasofacial angle were found. In addition, superior movement of the nasal tip was found, and as a result, the nasal hump decreased. The N-Sn/Pr ratio, nasal tip angle, and nasolabial angle did not show any significant changes. Conclusion: It can be concluded that more vertical nasal changes rather than sagittal nasal changes are observed after maxillary surgery.
Comparison of Microleakage of A Multi-Mode Adhesive System with Contemporary Adhesives in Class II Resin Restorations
(2014) Tuncer, Duygu; Celik, Cigdem; Cehreli, Sevi Burcak; Arhun, Neslihan; https://orcid.org/0000-0002-5936-0196; AAA-1576-2021; AAD-6138-2021; R-2536-2019
Aim: The aim was to compare the microleakage of resin composite bonded with different adhesive systems in class-II cavities at enamel or dentine margins. Material and methods: 60 extracted human molar teeth received slot cavity preparations on mesial and distal surfaces (mesial cervical margin was prepared in enamel and distal in dentine). They were randomly divided into five groups (n=12) according to the adhesive system: Group-A: Silorane Bond (S), Group-B: Adper Single Bond 2 (SB), Group-C: Clearfil SE Bond (CSE), Group-D: Single Bond Universal (USel) (selective etch-and-rinse), Group-E: Single Bond Universal (USE) (all-in-one). The preparations were restored using the same resin composite (Filtek Ultimate) except Group A which was restored by Silorane composite. The teeth were thermocycled, immersed in dye, sectioned, and dye penetration was evaluated quantitatively using image analysis. The data were analyzed using the two-way analysis of variance and Bonferroni test. Results: In all groups, there was no statistically significant difference between enamel margins at occlusal and gingival sites (p>0.05). The statistical difference between Group-A (S) and Group-B (SB) was significant at all margins. Group-B (SB) presented the greatest microleakage amounts at all margins and the highest scores were obtained in the dentine. Likewise, SB demonstrated statistically significant differences between dentine and enamel margins (occlusal and gingival)(p<0.05). Conclusion: All adhesive systems showed similar microleakage values between enamel margins in occlusal and gingival regions. However, when the gingival margin is located in the dentine, etch&rinse adhesive systems may not be a choice in terms of microleakage prevention.
Effects of different orthodontic adhesives and resin removal techniques on enamel color alteration
(2014) Boncuk, Yasemen; Cehreli, Zafer C.; Polat-Ozsoy, Omur
Objective: To investigate the color alterations in enamel following the use of different orthodontic bonding resins and adhesive residue removal burs. Materials and Methods: Metal brackets were bonded to extracted human premolars (n = 175) by using an etch-and-rinse adhesive system, a self-etch adhesive system (SEP), or a resin-modified glass ionomer cement (RMGIC). After 24 hours of photoaging, the brackets were removed and the adhesive residue on the tooth surfaces was cleaned with either a tungsten carbide bur or a Stainbuster bur. Tooth colors were measured with a spectrophotometer at baseline, after adhesive removal, and after additional photoaging. Color evaluation was made, and color differences induced by photoaging were calculated. Statistical evaluation was made using the Kruskal-Wallis test and the Mann-Whitney U-test, with Bonferroni correction. Results: All specimens showed discoloration at varying levels. The highest color change was observed in the etch-and-rinse adhesive/tungsten carbide bur group. When the etch-and-rinse and self-etch adhesives were used, adhesive-remnant removal with Stainbuster burs resulted in significantly lower discoloration. The type of bur did not affect the extent of enamel discoloration in the RMGIC group. Conclusions: Orthodontic treatment alters the original color of enamel, and both the adhesive system and the resin-removal methods are responsible for this change. When brackets are bonded with the etch-and-rinse system or the SEP, cleaning the adhesive residuals with Stainbuster burs is recommended for minimal change. RMGIC can be safely cleaned with tungsten carbide burs.
A Clinical Comparison of Cordless and Conventional Displacement Systems Regarding Clinical Performance and Impression Quality
(2014) Acar, Ozlem; Erkut, Selim; Ozcelik, Tuncer Burak; Ozdermr, Erdem; Akcil, Mehtap; https://orcid.org/0000-0001-5685-4409; 24360008; JJF-5618-2023
Statement of problem. it is not clear whether newly introduced cordless displacement systems are better able to manage gingiva than conventional systems. Purpose. The purpose of this in vivo study was to evaluate the gingival management ability of 4 different displacement methods with a standardized subgingival preparation finish line. Material and methods. The effects of 4 displacement techniques on gingival management and impression quality were evaluated by means of 6 evaluation criteria. A subgingival preparation finish line of between 1 and 2 mm was ensured, and the buccal aspects of 252 (n=63) teeth were clinically assessed for ease of application, time spent, bleeding, remnants, and dilatation. The complete reproduction of the preparation finish line and the bubble and void formations on polyether impressions were also evaluated. The data were statistically analyzed with the chi(2) test (alpha=.05). The Bonferroni correction was used to control Type I error for the pairwise comparison groups (alpha=.008). Results. Statistically significant differences were found for all criteria among the groups (P<.05). The nonimpregnated displacement cord group was the least effective group in terms of bleeding and impression quality (P<.008). The aluminum chloride impregnated cord group and the displacement paste with cap group were found to be comparable in terms of remnants, dilatation, and impression quality (P>.008). The retraction cap with paste group showed better results for ease of application, time spent, and bleeding than the aluminum chloride impregnated cord group (P<.008). Although the group with aluminum chloride impregnated cord, displacement paste, and cap showed better results for dilatation, it was time consuming and difficult (P<.008). Conclusions. Except for the nonimpregnated cord group, all of the groups were comparable and clinically useful, with perfect or acceptable impression qualities.
Microtensile Bond Strength of Composite-to-Composite Repair with Different Surface Treatments and Adhesive Systems
(2014) Celik, Cigdem; Cehreli, Burcak Sevi; Bagis, Bora; Arhun, Neslihan; https://orcid.org/0000-0002-5936-0196; AAA-1576-2021; R-2536-2019
Objectives: The purpose was to investigate the effect of different surface treatments and bonding agents on the repair bond strength of different resin-based restorative materials by microtensile bond strength (TBS) testing protocol. Materials and Methods: 24 Grandio SO(VOCO) and 24 Filtek Z250(3M) resin composite blocks were prepared. Half of the samples (N=12) were diamond bur-roughened and the other half (N=12) were sandblasted by 50m aluminum oxide particles. They were further divided into four sub-groups (n=3) and received the following: Sub-Group1: Adper Single Bond2 (Etch&Rinse) (3M); Sub-Group2: Clearfil SE (Self-etch) (Kuraray); Sub-Group3: Beauty Bond (HEMA-free all-in-one) (Shofu); Sub-Group4: All Bond3 (HEMA-free, hydrophobic, etch&rinse) (Bisco). The samples were repaired by Filtek Z250 to form a block. All of the resultant sub-groups combinations consisted of one of the composite type, surface treatment type, and adhesive systems. A total of 18 groups were prepared including 2 homogeneous blocks. They were thermocycled and TBS measurements were performed. Data were statistically analyzed with Kruskall-Wallis and Mann-Whitney U tests. Results: The experimental regroups' TBS reached to 34.67-66.36% and 43.44-95.52% of the cohesive bond strength for Grandio SO and Z250, respectively. The pre-existing composite type is found to be statistically important. When the surface is bur-finished Grandio performed better; when air-abrasion is considered Z250 showed higher bond strength. All-in-one adhesive system produced the weakest bond strength at all parameters. Conclusion: It may be suggested that when the pre-existing composite is unknown, air-abrasion may be performed with etch&rinse or two-step self-etch adhesives.
Management of Bisphosphonate-Related Osteonecrosis of the Jaw With a Platelet-Rich Fibrin Membrane: Technical Report
(2014) Soydan, Sidika Sinem; Uckan, Sina; 24075235
Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a challenging complication resulting from the long-term application of bisphosphonates. In most cases, BRONJ occurs after a surgical procedure involving the jawbone. Currently, the management of BRONJ remains controversial, and there is no definitive treatment other than palliative methods. Platelet-rich fibrin (PRF) represents a relatively new biotechnology for the stimulation and acceleration of tissue healing and bone regeneration. This technical note describes the total closure of moderate bone exposure in persistent BRONJ in 2 weeks with a double-layer PRF membrane. PRF may stimulate gingival healing and act as a barrier membrane between the alveolar bone and the oral cavity. PRF may offer a fast, easy, and effective alternative method for the closure of bone exposure in BRONJ. (C) 2014 American Association of Oral and Maxillofacial Surgeons
Hall Versus Conventional Stainless Steel Crown Techniques: in Vitro Investigation of Marginal Fit and Microleakage Using Three Different Luting Agents
(2014) Erdemci, Zeynep Yalcinkaya; Cehreli, S. Burcak; Tirali, R. Ebru; https://orcid.org/0000-0001-6487-3984; 25197992; AAD-6138-2021; HZK-4947-2023
Purpose: This study's purpose was to investigate microleakage and marginal discrepancies in stainless steel crowns (SSCs) placed using conventional and Hall techniques and cemented with three different luting agents. Methods: Seventy-eight human primary maxillary second molars were randomly assigned to two groups (N=39), and SSCs were applied either with the Hall or conventional technique. These two groups were further sub grouped according to the material used for crown cementation (N=13 per group). Two specimens in each group were processed for scanning electron microscopy investigation. The extent of microleakage and marginal fit was quantified in millimeters on digitally photographed sections using image analysis software. The data were compared with a two-way independent and a two-way mixed analysis of variance (P=.05). Results: The scores in the Hall group were significantly worse than those in the conventional technique group (P<.05). In both groups, resin cement displayed the lowest extent of microleakage, followed by glass ionomer and polycarboxylate cements (P<.05). Conclusions: Stainless steel crowns applied using the Hall technique displayed higher microleakage scores than those applied using the conventional technique, regardless of the cementation material. When the interaction of the material and technique was assessed, resin cement presented as the best choice for minimizing microleakage in both techniques.
Use of Cone-Beam Computed Tomography in Diagnosis of An Otherwise Undetected Periapical Lesion in An Anomalous Tooth
(2014) Gulsahi, Ayse; Ates, Ufuk; Tirali, Resmiye Ebru; Cehreli, Sevi Burcak; https://orcid.org/0000-0001-6487-3984; HGA-3970-2022; AAD-2907-2020; AAD-6138-2021
Fusion is a developmental anomaly of dental hard tissues. Since dental fusion is characterized by irregular coronal morphology and a complex endodontic anatomy, endodontic therapy of such teeth may present a serious clinical challenge. Cone-beam computed tomography (CBCT) is a useful tool for the management of complex endodontic problems and dental anomalies. In the case presented here, a CBCT scan revealed morphological details as well as the severity of periapical infection that had not been visualized with conventional imaging techniques. The results obtained with detailed imaging led to a change in the treatment plan.
Depigmented skin and phantom color measurements for realistic prostheses
(2014) Tanner, Paul; Leachman, Sancy; Boucher, Kenneth; Ozcelik, Tuncer Burak
PurposeThe purpose of this study was to test the hypothesis that regardless of human skin phototype, areas of depigmented skin, as seen in vitiligo, are optically indistinguishable among skin phototypes. The average of the depigmented skin measurements can be used to develop the base color of realistic prostheses. Methods and MaterialsData was analyzed from 20 of 32 recruited vitiligo study participants. Diffuse reflectance spectroscopy measurements were made from depigmented skin and adjacent pigmented skin, then compared with 66 pigmented polydimethylsiloxane phantoms to determine pigment concentrations in turbid media for making realistic facial prostheses. ResultsThe Area Under spectral intensity Curve (AUC) was calculated for average spectroscopy measurements of pigmented sites in relation to skin phototype (P=0.0505) and depigmented skin in relation to skin phototype (P=0.59). No significant relationship exists between skin phototypes and depigmented skin spectroscopy measurements. The average of the depigmented skin measurements (AUC 19,129) was the closest match to phantom 6.4 (AUC 19,162). ConclusionAreas of depigmented skin are visibly indistinguishable per skin phototype, yet spectrometry shows that depigmented skin measurements varied and were unrelated to skin phototype. Possible sources of optical variation of depigmented skin include age, body site, blood flow, quantity/quality of collagen, and other chromophores. The average of all depigmented skin measurements can be used to derive the pigment composition and concentration for realistic facial prostheses.
The Effect of Temperature and Contact Time of Sodium Hypochlorite on Human Roots Infected with Enterococcus Faecalis and Candida Albicans
(2014) Gulsahi, Kamran; Tirali, R. Ebru; Cehreli, S. Burcak; Karahan, Zeynep Ceren; Uzunoglu, Emel; Sabuncuoglu, Bizden; https://orcid.org/0000-0003-3510-7265; https://orcid.org/0000-0001-6487-3984; 23011474; AAX-5565-2021; AAD-2907-2020; AAD-6138-2021
The aim of this study was to evaluate the effectiveness of 2.5 % NaOCl at different temperature and time intervals on Enterococcus faecalis and Candida albicans-infected human roots. A total of 112 root cylinders prepared from extracted single-rooted humans were infected by E. faecalis (Group A, n = 56) or C. albicans (Group B, n = 56); 3 root cylinders served as negative controls. Both groups were further divided into 6 subgroups according to three contact times (30 s, 1 min, 5 min) with NaOCl at two different temperatures (25 or 37 A degrees C). Microorganism growth was controlled at the 24th and 48th hours. Statistical analysis was performed using the Chi-square test. While NaOCl at 25 A degrees C for 5 min was the most effective irrigation regimen to eliminate E. faecalis (p < 0.001), NaOCl at 37 A degrees C for 5 min exhibited significantly superior antifungal properties (p < 0.05). At the same contact times, difference in the temperature of NaOCl did not affect the growth of either E. faecalis or C. albicans. As a result, the irrigation time of NaOCl was more effective than the temperature to eliminate E. faecalis, while pre-heating of NaOCl to 37 A degrees C increased its effectiveness on C. albicans at 5 min contact time.
Comparative Treatment Outcomes of Menopausal and Nonmenopausal Women After Arthrocentesis
(2014) Ungor, Cem; Cezairli, Burak; Taskesen, Fatih; Dayisoylu, Ezher Hamza; Senel, Figen Cizmeci; https://orcid.org/0000-0002-2899-2836; https://orcid.org/0000-0002-1196-9840; 000345012000031; AAN-7097-2021; ADG-1958-2022
Objectives: Most of the patients treated for temporomandibular joint disorders constituted from the female population, and this situation is generally hypothesized and demonstrated in several studies. Arthrocentesis of the temporomandibular joint is a minimally invasive and effective treatment method in the management of patients with stage III and stage IV Wilkes syndrome. This study was aimed at assessing the effectiveness of this method between menopausal and nonmenopausal female patients. Study Design: A total of 59 patients (25 menopausal and 34 nonmenopausal) were evaluated according to the visual analog scale for pain and chewing function scores, joint sound, and maximum mouth opening. Results: In both groups, the parameters were detected statistically significant according to follow-up periods. The menopausal group showed better results than the nonmenopausal group at 6-month controls. Conclusion: In conclusion, arthrocentesis is a satisfactory treatment modality in themanagement of temporomandibular joint derangements. Female reproductive hormone levels may affect the success of this procedure.
Effect of Adhesive Systems on Microleakage in Endodontically Treated Teeth Restored with Glass Fiber Posts
(2014) Ozdemir, Erdem; Gulsahi, Kamran; Korkmaz, Yonca; Erkut, Selim; Tuncer, Abidin Hakan; https://orcid.org/0000-0003-3510-7265; AAX-5565-2021
The effect of adhesive systems on microleakage in endodontically treated teeth restored with glass fiber posts was studied. Forty carious and crack-free premolars were prepared using a stepback technique and obturated with gutta-percha. The endodontically treated roots were randomly divided into four groups (n = 10). Glass fiber posts cemented following adhesive luting agents; group 1: All Bond 3/Duo Link (ALB/DL), group 2: One-Step Plus/Duo-Link (OSP/DL), group 3: Panavia F 2.0/Panavia ED Primer II (PAN/ED), and group 4: Rely X Unicem (RU). All specimens were thermal cycled and stored in basic fuchsin solution for one week. The roots were sectioned horizontally into three sections: apical, middle, and coronal. The occlusal direction of each section was digitally photographed under a stereomicroscope. Dye penetration area was calculated as the basic fuchsin infiltrated surface divided by total dentin. The data were statistically analyzed using the Mann-Whitney U and Wilcoxon Signed Ranks Tests (alpha = 0.05). The mean area measurements were ranged between 0 and 3.7357. According to the Mann-Whitney U test, there was no significant difference among the groups and sections (p > 0.05). Intragroup comparisons showed a significant difference between coronal-apical and middle-coronal sections in Panavia group (p = 0.043) by Wilcoxon Signed Test. It was found that there was no significant difference among four groups and the apical, coronal, and middle sections. The only difference was found for the group of PAN/ED between coronal-apical and coronal-middle sections.
Does an Alkaline Environment Prevent the Development of Bisphosphonate-Related Osteonecrosis of the Jaw? An Experimental Study in Rats
(2014) Dayisoylu, Ezher H.; Ungor, Cem; Tosun, Emre; Ersoz, Safak; Duman, Mine Kadioglu; Taskesen, Fatih; Senel, Figen Cizmeci; 24368141
Objective. To investigate the preventive effect of locally applied sodium bicarbonate on bisphosphonate-related osteonecrosis of the jaw (BRONJ). Study Design. Thirty-six Sprague-Dawley rats were divided into 4 groups. Animals in group I received 0.1 mg/ kg sterile saline 3 times per week for 8 weeks. Groups II, III, and IV received intraperitoneal zoledronate injection in the same manner with the same frequency and duration. The right first molar tooth was extracted in groups III and IV. One mL 8.4% sodium bicarbonate (SB) was applied to the extraction socket at the time of extraction in group IV. The effect of locally applied SB as an alkalizing agent was evaluated by histomorphometric analysis. Results. BRONJ was observed in none of the animals in the control groups, 67% of the animals in the tooth extraction group, and none of the animals in the local SB application group (P < .01). Conclusions. Administration of locally applied SB had positive effects on the prevention of BRONJ in animals, but further studies are required to verify the effectiveness of this form of treatment before its use in humans.
Effects of various analgesics on the level of prostaglandin E2 during orthodontic tooth movement
(2014) Tuncer, Zeynep; Polat-Ozsoy, Omur; Demirbilek, Muge; Bostanoglu, Ebru
AIM: The aim of this double-blind, randomized, placebo-controlled clinical study was to evaluate the analgesic effects of preoperative/postoperative ibuprofen and acetaminophen use after bonding and to find a relation between the pain level and the amount of prostaglandin released. MATERIALS AND METHODS: Forty-eight patients were included and randomly divided to three equal groups that received either ibuprofen, acetaminophen or placebo for pain relief. The pain levels were measured before bonding, after bonding, at first, second, third, and seventh days on a 100 mm visual analogue scale (VAS) and gingival crevicular fluid (GCF) samples were collected at the same time intervals to measure the amount of prostaglandin E2 (PGE2) released. PGE2 levels were determined with ELISA test. The results were evaluated with Wilcoxon and Kruskal-Wallis tests with Bonferroni correction. RESULTS: Acetaminophen and placebo groups showed similar pain levels during the first 2 days, whereas ibuprofen group showed lower pain levels during the first day after bonding. PGE2 levels did not show statistically significant difference in time within the analgesic groups. No significant relation between the pain perceived and PGE2 released was found. LIMITATIONS: The biggest limitation of this study is the subjective nature of pain and its method of evaluation. CONCLUSIONS: The perception of pain by patients taking ibuprofen and acetaminophen at pre/post appliance placement was not different from patients taking placebo. No time-related differences in PGE2 level were found between the groups and no significant correlation was found between the perception of pain and PGE2 levels.
Effect of Different Surface-Cleaning Techniques on The Bond Strength of Composite Resin Restorations
(2014) Erkut, Selim; Yilmaz, Burak; Bagis, Bora; Kucukemen, Cigdem; Ozdemir, Erdem; Acar, Ozlem; 24939254
Statement of problem. Different techniques have been suggested for cleaning dentin surfaces after the removal of an interim prosthesis and before the application of a bonding agent. How different surface-cleaning techniques affect the bond strength of the composite resin restorations is not clear. Purpose. The purpose of this study was to investigate the effects of different surface-cleaning techniques on the bond strength of composite resin restorations and the surface topography of the prepared tooth surfaces. Material and methods. The occlusal surfaces of 25 molars were ground until the dentin was exposed. A bonding agent and interim cement were applied on the teeth. The teeth were divided into 5 groups (n=5) according to the method used for surface-cleaning (microairborne-particle abrasion, alcohol, rubber-rotary instrument, desiccating agent, and control). Once the surfaces of the teeth had been cleaned, the same bonding material was applied to the teeth. A 5-mm-thick composite resin layer was built up. Each specimen was sectioned to microbars, and 6 centrally located beams were selected for microtensile testing (n=30) (1.10 +/- 0.10 mm). The data were statistically analyzed with 1-way ANOVA (1-sample Kolmogorov-Smirnov test). The Bonferroni test was used for significantly different groups (alpha=.05). One specimen from each group was observed under a scanning electron microscope and an atomic force microscope. Energy dispersive x-ray analysis also was performed. Results. Bond strength values were in the following descending order: microairborne-particle abrasion, desiccating agent, alcohol, rubber-rotary instrument, control. Differences between the microairborne-particle abrasion group and the remainder of the groups, desiccating agent-rubber-rotary instrument, desiccating agent-control, alcohol-rubber-rotary instrument, and alcohol-control groups, were statistically significant (P<.05). The microairborne-particle abrasion group displayed the roughest surface and a different surface topography from the remainder of the groups. Increased aluminum was observed in the microairborne-particle abrasion group. Conclusions. Surface-cleaning techniques, except for the rubber-rotary instrument, increased the bond strength of composite resin. The roughest dentin surfaces and highest bond strength were achieved with the microairborne-particle abrasion technique.
Efficiency of pivot splints as jaw exercise apparatus in combination with stabilization splints in anterior disc displacement without reduction: a retrospective study
(2014) Muhtarogullari, Mehmet; Avci, Mehmet; Yuzugullu, Bulem; 25300939
Objective: To evaluate efficiency of pivot splints in jaw exercises, in combination with stabilization splints, in cases of anterior disc displacement without reduction of temporomandibular joint. Subjects and methods: Twenty-three patients who referred to the prosthodontics clinic in 1995-1997 were included in the study, where anterior disc displacement without reduction of temporomandibular joint was diagnosed using magnetic resonance imaging and clinical examination. Pivot splints were used for jaw exercises for five minutes long; five times/day and stabilization splints were used at all other times. The patients were followed for 24 weeks. Lateral and protrusive excursions along with maximum mouth opening and were evaluated at each control. Bilateral palpation of temporal, masseter, sternocleidomastoid muscles and TMJ was assessed for pain perception before and after treatment. Data were statistically analyzed using Paired sample t-test and Independent Samples t-test (p < .05). Results: Mean mandibular range of motion measurements increased from 28.74 mm prior to 49.17 mm on maximum opening; right/left lateral excursion from 7.61 mm to 12.04 mm and 4.09 mm to 7.3 mm on protrusion after treatment. All changes observed before and after treatment were found to be statistically significant. (p < .001) Pain symptoms were eliminated at the end of 24 weeks of treatment in all patients. Conclusion: Using pivot splints as an exercise regimen along with a stabilization splint may be a viable treatment option for patients with anterior disc displacement without reduction; as normal mandibular range of motion was established and pain was eliminated.
Is The Incidence of Temporomandibular Disorder Increased in Polycystic Ovary Syndrome?
(2014) Soydan, Sidika Sinem; Deniz, Kagan; Uckan, Sina; Unal, Asli Dogruk; Tutuncu, Neslihan Bascil; https://orcid.org/0000-0002-3798-7326; https://orcid.org/0000-0002-1816-3903; 25124832; ABG-5027-2020
The prevalence of temporomandibular disorders is higher among women than men (ratio 3:1 -9:1). Polycystic ovary syndrome(PCOS) is the most common endocrine disorder in women, which is characterised by chronic low-grade inflammation and excess of androgenic hormones that lead to metabolic aberrations and ovarian dysfunction. Increased activities of various matrix metalloproteinases (particularly MMP-2 and 9) in the serum of these patients has been reported, and it has been hypothesised that high activities of MMP may contribute to loss of matrix and chronic inflammation of the fibrocartilage in temporomandibular disorders. Our aim was to evaluate the incidence of temopormandibular dysfunction in women with PCOS compared with an age-matched, disease-free, control group. We studied 50 patients with previously diagnosed PCOS and 50 volunteers who had normal menstrual cycles. We made a comprehensive clinical examination of the temporomandibular joint (TMJ) and muscles of mastication in both groups and recorded the Visual Analogue Scores (VAS) for pain. There were significant differences (p<0.001) in the incidence of temporomandibular disorders (n=43 (86%) in the PCOS group compared with n=12 24% in the control group), muscle tenderness(n=32 (64%) in the PCOS group compared with n=14 (28%) in the control group) and pain in the TMJ (mean (SD) VAS 2.9 (2.61) compared with 0.3 (1.56). We confirm the higher incidence and severity of disorders of the TMJ in patients with PCOS and suspect that chronic low-grade inflammation may play a part in the aetiology of the disease. Copyright (C) 2014 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
Interdisciplinary Management of an Adult Patient with A Class III Malocclusion
(2014) Pektas, Zafer Ozgur; Kircelli, Beyza Hancioglu; 24370388
The coexistence of a skeletal class III deformity and partial edentulism poses a clinical challenge and requires a comprehensive evaluation followed by a multidisciplinary approach. This clinical report presents the integrated management of a class Ill malocclusion in a 50-year-old woman with partial edentulism. The patient received adjunctive orthodontic treatment with a rigid temporary anchorage device, a Le Fort I maxillary osteotomy, and prosthodontic rehabilitation with removable maxillary copings, an overdenture, and a mandibular partial removable dental prosthesis with precision attachments.