In Vivo Study of The Role of Hyaluronic Acid, N-Acetyl Cysteine, and Deproteinized Calf Serum on Injury-Induced Cartilage Degeneration

Abstract

Objectives: The aim of this study was to compare the effects of hyaluronic acid ( HA), N-acetyl cysteine ( NAC), and deproteinized calf serum on cartilage healing after the creation of traumatic cartilage injury in a rat model.

Materials and methods: A total of 48 rats, each weighing an average of 350 g, were randomly separated into four groups of 12. An osteochondral defect was created, 2-mm-wide and 3-mm deep in each rat. Injections were made to the knees of the rats as saline solution in Group 1, deproteinized calf serum in Group 2, NAC in Group 3, and HA in Group 4. At the end of 12 weeks, all rats were sacrificed and tissues were evaluated histologically.

Results: The HA group had a better cell morphology, tissue morphology, surface architecture, and vascularity than the other groups (p < 0.001). Matrix staining, chondrocyte clustering, and the assessment scores of the mid, deep, superficial zones, and overall were higher in the HA group than in the other groups (p < 0.001). The NAC showed a better tissue morphology, cell morphology, and vascularity than the control group (p= 0.003, p < 0.001, and p < 0.001, respectively).

Conclusion: Hyaluronic acid was the most effective agent in cartilage healing compared to NAC and deproteinized calf serum. In addition, the NAC was more effective compared to the control group.