A Novel Simplified Combination of Monoclonal Antibodies for Flow Cytometric Analysis of Bronchoalveolar Lavage Samples

dc.contributor.authorPepedil-Tanrikulu, Funda
dc.contributor.authorSen, Nazan
dc.contributor.authorBuyukkurt, Nurhilal
dc.contributor.authorSariturk, Cagla
dc.contributor.authorKozanoglu, Ilknur
dc.contributor.pubmedID31532092en_US
dc.date.accessioned2020-12-15T07:59:07Z
dc.date.available2020-12-15T07:59:07Z
dc.date.issued2019
dc.description.abstractBackground: The profile of leukocytes in bronchoalveolar lavage (BAL) fluid provides important information for diagnosing various lung diseases. A differential cell count of BAL is conventionally performed by evaluating centrifuged samples under a light microscope and enumerating the stained cells. Another rarely used method to identify BAL leukocytes is flow cytometry (FCM). However, there are no guidelines for standardizing this method and related literature is limited. This study aimed to evaluate the accuracy of FCM for identifying BAL leukocytes. Methods: The BAL samples accepted to the hematology laboratory between 2014 - 2018 were retrospectively evaluated via light microscopy (LM) by a hematologist; while flow cytometric analyses with a monoclonal antibody panel composed of CD45/CD14/CD16 were noted by another doctor. The percentages of macrophages, lymphocytes, neutrophils and eosinophils determined by both methods were recorded for analysis. Correlations between the results from LM and FCM were investigated. In addition, compatibility between LM and FCM for denoting pathological values for each cell type was checked. Results: Among 140 reviewed BAL samples, 76 were included for further analysis. Comparisons revealed strong correlations between FCM and LM for identifying macrophages, lymphocytes, neutrophils, and eosinophils. In addition, regarding the normal cutoff values for each leukocyte type, FCM and LM were similar in the identification of pathological changes of all cell types except eosinophils. Conclusions: Flow cytometry was found to be feasible for use instead of LM and might become a more widely used technique to analyze BAL fluid in the future.en_US
dc.identifier.endpage1621en_US
dc.identifier.issn1433-6510en_US
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-85072271219en_US
dc.identifier.startpage1615en_US
dc.identifier.urihttp://hdl.handle.net/11727/5033
dc.identifier.volume65en_US
dc.identifier.wos000486387200005en_US
dc.language.isoengen_US
dc.relation.isversionof10.7754/Clin.Lab.2019.190131en_US
dc.relation.journalCLINICAL LABORATORYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergien_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbronchoalveolar lavageen_US
dc.subjectflow cytometryen_US
dc.subjectlight microscopyen_US
dc.titleA Novel Simplified Combination of Monoclonal Antibodies for Flow Cytometric Analysis of Bronchoalveolar Lavage Samplesen_US
dc.typearticleen_US

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