Fakülteler / Faculties
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Item Design and Construction of Affordable and Domestic Device for Carbon Nanoparticle Synthesis(2018) Aykutlu, Dilara; Okursoy, Berkay; Kocum, Ismail Cengiz; Cokeliler Serdaroglu, DilekNanoparticles are structures below 100 nanometer and used for molecular imaging with gene therapy, implementation of many biomedical applications such as biosensor, cancer-pathology diagnosis and treatment, targeted drug-making and therefore superior in application areas. The aim of the study is to develop a device that synthesizes carbon nanoparticles by dense medium plasma method; together with hardware, software and mechanical design. Dense medium plasma is an easy synthesis approach with low cost; However, it is necessary for the user of this device to be more comfortable to use, feasible, portable and suitable for market. Moreover there is no commercial device for synthesing carbon nanoparticles by dense medium plasma technique. This research presents details of construction of affordable device for carbon nanoparticle sythesis. Prototype is an unique domestic product and contains integrated hardware, software and mechanical probe parts. Hardware part consists of control, power unit and induction coil unit and these three units are connected to each other. The power unit ensures that the mosfets are triggered by a certain voltage from the control unit. The induction coil unit is induced by turning the low voltage to high voltage. In the mechanical part, probe design supplies discharge betweeen electodes which are fed by argon gase that converts benzene to the carbon nanoparticles. The probe reaches the resonance frequency and to make it easier to adjust the distance between the bottom and top electrode, a spindle system is used. Software operation is measuring high voltage. To make the appliance portable, moreover wheeled table production was done. As a result, signal and power card tests were made, it was observed that the high voltage circuit, the signal circuit and the induction coil cause radiation. More nanoparticle production was achieved with the controllable distance between the bottom electrode and the top electrode of probe. The efficiency of the synthesis was improved and the usage of the device was made practical. Finally prototype device that is usable for synthesis nanoparticles by dense medium technology is constructed affordablity and presented with all details.Item Magnetically responsive, sorafenib loaded alginate microspheres for hepatocellular carcinoma treatment(2020) Alpdemir, Sukran; Vural, Tayfun; Kara, Goknur; Bayram, Cem; Haberal, Erdem; Denkbas, Emir Baki; 0000-0003-2788-550X; 33010138; ABC-8833-2020This study aimed to develop sorafenib loaded magnetic microspheres for the treatment of hepatocellular carcinoma. To achieve this goal, superparamagnetic iron oxide nanoparticles (SPIONs) were synthesised and encapsulated in alginate microspheres together with an antineoplastic agent, sorafenib. In the study, firstly SPIONs were synthesised and characterised by dynamic light scattering, energy-dispersive X-ray spectroscopy, and scanning electron microscopy. Then, alginate-SPIONs microspheres were developed, and further characterised by electron spin resonance spectrometer and vibrating sample magnetometer. Besides the magnetic properties of SPIONs, alginate microspheres with SPIONs were also found to have magnetic properties. The potential use of microspheres in hyperthermia treatment was then investigated and an increase of about 4 degrees C in the environment was found out. Drug release studies and cytotoxicity tests were performed after sorafenib was encapsulated into the magnetic microspheres. According to release studies, sorafenib has been released from microspheres for 8 h. Cytotoxicity tests showed that alginate-SPION-sorafenib microspheres were highly effective against cancerous cells and promising for cancer therapy.Item Penetration Depth in Nanoparticles Incorporated Radiofrequency Hyperthermia into the Tissue: Comprehensive Study with Histology and Pathology Observations(2019) Nasseri, Behzad; Kocum, Ismail Cengiz; Seymen, Cemile Merve; Rebiee, Navid; 31432798In present study, the effective penetration of radiofrequency (RF) induced gold decorated iron oxide nanoparticles (GS@IONPs) hyperthermia was investigated. The effective penetration depth of RF also the damage potency of hyperthermia was evaluated during histopathology observations which were done on the chicken breast tissue and hepatocellular carcinoma (HCC) models. The thermal damages are well- documented in our previous cellular study which was engaged with potency of RF hyperthermia in Epithelial adenocarcinoma (MCF-7) and fibroblast (L-929) cells deaths [1]. In recent work, PEGylated iron oxide nanoparticles (IONPs) were used as base platform for gold magnetic nanoparticles (GS@IONPs) formation. The 144.00015 MHz, 180W RF generator was applied for stimulating the nanoparticles. The chicken breast tissue and the hepatocellular tumor model was considered in the experimental section. In histology studies, the structural changes also the effective penetration depth of RF induced nanoparticles was observed through microscopic monitoring of the tissue slices in histology observations (Gazi medical school). The highest damage level was seen in 8.0 mu m tissue slices where lower damages were seen in depth of 1.0 cm and more inside tissue. The histology observations clarified the effective penetration depth of RF waves and irreversible damages in the 2.0 cm inside the tissue.Item Designing siRNA-conjugated plant oil-based nanoparticles for gene silencing and cancer therapy(2019) Anilmis, Nur Merve; Kara, Goknur; Kilicay, Ebru; Hazer, Baki; Denkbas, Emir Baki; 31509450In this study, the anticancer activities of two siRNA carriers were compared using a human lung adenocarcinoma epithelial cell line (A549). Firstly, poly(styrene)-graft-poly(linoleic acid) (PS-g-PLina) and poly(styrene)-graft-poly(linoleic acid)-graft-poly(ethylene glycol) (PS-g-PLina-g-PEG) graft copolymers were synthesized by free-radical polymerization. PS-PLina and PS-PLina-PEG nanoparticles (NPs) were prepared by solvent evaporation method and were then characterized. The size was found as 150 +/- 10 nm for PS-PLina and 184 +/- 6 nm for PS-PLina-PEG NPs. The NPs were functionalized with poly(l-lysine) (PLL) for c-myc siRNA conjugation. siRNA entrapment efficiencies were found in the range of 4-63% for PS-PLina-PLL and 6-42% for PS-PLina-PEG-PLL NPs. The short-term stability test was realised for 1 month. siRNA release profiles were also investigated. In vitro anticancer activity of siRNA-NPs was determined by MTT, flow cytometry, and fluorescence microscopy analyses. Obtained findings showed that both NPs systems were promising as siRNA delivery tool for lung cancer therapy.