Fakülteler / Faculties

Permanent URI for this communityhttps://hdl.handle.net/11727/1395

Browse

Filters

2020 - 20242

Settings

Subject: search.filters.subject.caffeic acid

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Tumour-Specific Hybrid Nanoparticles In Therapy Of Breast Cancer
    (JOURNAL OF MICROENCAPSULATION, 2024-01-02) Kilicay, E.; Erdal, E.; Elci, P.; Hazer, B.; Denkbas, E. B.
    In this study, salicylic acid (SA) dopped into poly(3-hydroxybutyrate) (PHB) and prepared nanoparticles (NPs) to increase encapsulation efficiency, anti-cancer activity of caffeic acid (Caff), and folic acid (FA) for breast cancer treatment. NPs were prepared by solvent evaporation method and characterised by FTIR, DSC, SEM, and entrapment-loading efficiencies. The mean diameter, polydispersity index (PDI), and zeta potential (ZP) were evaluated by dynamic light scattering (DLS). In vitro release and stability studies were done via eppendorf method. The cytotoxicity, cell dead and internalisation of NPs were shown by MTT, fluorescein and confocal microscopy. The diameter and ZP of NPs were 172 +/- 7 nm and -29 +/- 0.38 mV. The entrapment efficiencies of 5 and 10 Caff NPs were 79 +/- 0.23% and 70 +/- 0.42%. NPs showed good stability within 30 d and sustained release over 25 d. FA-5Caff NPs showed 37 +/- 0.3% viability on MCF-7. FA-Caff NPs were identified as promising carrier system for breast cancer therapy.
  • No Thumbnail Available
    Item
    The preparation of chitosan membrane improved with nanoparticles based on unsaturated fatty acid for using in cancer-related infections
    (2020) Kizaloglu, Abdullah; Kilicay, Ebru; Karahaliloglu, Zeynep; Hazer, Baki; Denkbas, Emir Baki
    This study includes the design of a chitosan membrane decorated with unsaturated fatty acid-based carrier system for cancer treatment and antibacterial application. For this, polystyrene-graft-polyoleic acid-graft-polyethylene glycol was prepared by free radical polymerization and characterized. Nanoparticles and caffeic acid-loaded nanoparticles were prepared by solvent evaporation technique and optimized. The short-term stability of nanoparticles was investigated at 4 degrees C. Drug encapsulation and loading efficiency were evaluated. The chitosan membrane and caffeic acid-loaded nanoparticles embedded into chitosan membrane were fabricated. The caffeic acid loaded nanoparticles embedded into chitosan membrane showed controlled release. The mechanical properties of all samples were investigated. The caffeic acid-loaded nanoparticles embedded into chitosan membranes indicated excellent antibacterial properties against theEscherichia coliandStaphylococcus aureus. The anticancer activity of all the samples was evaluated against SaOS-2 human primary osteogenic sarcoma and MC3T3-E1 pre-osteoblast cell lines by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, the flow cytometry and double staining methods. As a result, the designed carrier system showed great potential to cancer-associated infections treatment in bone cancer cases.