Diş Hekimliği Fakültesi / Faculty of Dentistry

Permanent URI for this collectionhttps://hdl.handle.net/11727/2120

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    Effects of a Cyclic NSAID Regimen on Levels of Gingival Crevicular Fluid Prostaglandin E-2 and Interleukin-1 beta: A 6-month Randomized Controlled Clinical Trial
    (2018) Oduncuoglu, B. F.; Kayar, N. A.; Haliloglu, S.; Serpek, B.; Ataoglu, T.; Alptekin, N. O.; https://orcid.org/0000-0003-0647-9481; https://orcid.org/0000-0003-4104-6462; 29735869; AAQ-4792-2020; G-1816-2014
    Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for inflammation control and pain relief. However, while the adjunct use of NSAIDs is avoided for periodontal therapy because of related side effects, cyclic administration of NSAIDs may reduce or eliminate these effects. We evaluated the effect of a cyclic diclofenac potassium (DP) regimen on clinical parameters and levels of prostaglandin E-2 (PGE(2)) and interleukin-1 beta (IL-1 beta) in the gingival crevicular fluid (GCF) of individuals with periodontitis. Materials and Methods: The study protocol was approved by the Ethics Committee (2000/071). Forty-one individuals with chronic periodontitis (33 men, 8 women) were divided into two groups (test and control) after initial periodontal therapy. During this 6-month, randomized, double-blind, placebo-controlled study, test (n = 28) and control (n = 13) groups were administered a cyclic regimen of DP (50 mg, twice daily) or placebo. Clinical measurements and GCF sample collections were made at baseline, 2, 4, and 6 months. GCF levels of PGE(2) and IL-1 beta were determined using enzyme immunoassay and enzyme-linked immunoassay kits, respectively. Results: At baseline, no significant differences existed between groups for plaque indices, gingival indices, bleeding on probing, probing depth (PD), or attachment levels (P > 0.05). Compared with the control group, cyclic regimen in the test group suppressed increased levels of PGE(2) found in GCF at the end of the study (P < 0.05). Significant differences for PD and relative attachment gain were also noted in favor of the test group (P < 0.05). Conclusions: These results suggest that a cyclic regimen of DP may be efficacious in the management of chronic periodontitis in adults.
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    Salivary Del-1, IL-17, and LFA-1 levels in periodontal health and disease
    (2020) Inonu, Elif; Kayis, Seyit Ali; Eskan, Mehmet Akif; Hakki, Sema S.; 32153040
    Objective and Background Developmental endothelial locus-1 (Del-1), lymphocyte function-associated antigen-1 (LFA-1), and interleukin 17 (IL-17) play critical roles in transendothelial migration of neutrophils in periodontal diseases. The aim of this study was to evaluate salivary Del-1, IL-17, and LFA-1 protein levels in patients with gingivitis (G), chronic periodontitis (CP), and generalized aggressive periodontitis (GAP). Methods A total of 180 systemically healthy, non-smoking patients (45 periodontally healthy (H) and 45 G, 50 CP, and 40 GAP) individuals (between March 2014 and February 2016) were included in this study according to Armitage's (1999) classification. Clinical periodontal parameters, including clinical attachment level, probing depth, plaque index, and gingival index, were recorded. Del-1, IL-17, and LFA-1 protein expression levels were measured in unstimulated saliva samples collected from patients by using enzyme-linked immunosorbent assays.Kruskal-WallisandMann-Whitney U testswere used for multiple comparisons and post hoc statistical analyses, respectively. ROC curve analysis was used to evaluate the sensitivity and specificity of Del-1, IL-17, and LFA-1 in distinguishing periodontal disease from health and gingivitis. Results It was found a high level of IL-17 and a low level of Del-1 in the CP and GAP, as compared to the G and H groups (P < .001). Nevertheless, we found LFA-1 levels were higher in the GAP than in the CP or G groups (P = .00). Consistently, LFA-1 levels were lower in the H and G groups than in the CP and GAP groups (P = .00). The combination of three biomarkers was found as the best predictor yielded exhibited the highest AUC [0.893, 0.845-0.94 (%95 CI)P < .001] in discriminating periodontal disease from health and gingivitis. Conclusion Salivary Del-1, LFA-1, and IL-17 levels might be useful markers for determining the clinical health and disease status of patients with periodontitis. However, further studies that evaluate the level of salivary Del-1, LFA-1, and IL-17 before and after periodontal therapy are required to understand the exact roles of these cytokines during the periodontal healing period.