Image Analysis For Single Cell Gel Electrophoresis

dc.contributor.authorMese, Alev Kakac
dc.contributor.authorErdamar, Aykut
dc.contributor.authorIseri, Ozlem Darcansoy
dc.contributor.orcID0000-0001-8588-480Xen_US
dc.contributor.researcherIDAAA-6844-2019en_US
dc.date.accessioned2023-06-08T08:19:39Z
dc.date.available2023-06-08T08:19:39Z
dc.date.issued2017
dc.description.abstractDetection of dioxyribonucleic acid (DNA) damage is very crucial in various areas of life sciences and in the clinical diagnosis of some pathophysiologies. Single cell gel electrophoresis, also called Comet Assay, is a reliable and easily applicable method to measure/detect level of DNA damage which is an indicator of an genotoxic and cytotoxic effect on living organisms caused by chemical and phsyical activity. The method is generally based on the fact that the DNA in the nucleus isolated from living tissues is placed in a thin agarose gel and run on an electrophoretic medium. DNA images obtained with the Comet protocol can be evaluated visually as well as can be analyzed using various software today. With such software, objective results can be obtained in a short period of time and without adhering to the researcher's experience. In this study, Comet analysis images obtained from HepG2 (ATCC HB-8065) hepatocellular carcinoma liver cancer cells were used. Calculation of measurement results of these user-selected images and presenting parametric data to the user are intended.en_US
dc.identifier.issn2165-0608en_US
dc.identifier.scopus2-s2.0-85026323471en_US
dc.identifier.urihttp://hdl.handle.net/11727/9441
dc.identifier.wos000413813100279en_US
dc.language.isoturen_US
dc.relation.journal25th Signal Processing and Communications Applications Conference (SIU)en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectComet Assayen_US
dc.subjectDNA Damageen_US
dc.subjectImage Analysisen_US
dc.subjectOtsu Methoden_US
dc.titleImage Analysis For Single Cell Gel Electrophoresisen_US
dc.typeconferenceObjecten_US

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