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Item New-onset cutaneous nodule arising from hypertrichotic brownish patch in a 6-year-old girl(2022) Incel Uysal, Pinar; Terzi, Aysen; Uysal, Ahmet Cagri; 35636973Item The Effect of Adipose Derived Stromal Vascular Fraction on Flap Viability in Experimental Diabetes Mellitus and Chronic Renal Disease(2022) Ozkan, Burak; Eyuboglu, Atilla Adnan; Terzi, Aysen; Ozturan Ozer, Eda; Tatar, Burak Ergun; Uysal, Cagri A.; 0000-0003-3093-8369; 35450516Background The presence of chronic renal disease(CRD) concurrently with diabetes mellitus(DM) increases the flap failure. Adipose derived stromal vascular fraction (SVF) is known to enhance skin flap viability in both healthy and diabetic individuals. The aim of this experimental study was to investigate the effect of SVF on skin flap viability in rats with DM and CRD. Methods 48 Sprague-Dawley rats were separated into four groups as follows: group I (control), group II (diabetes mellitus), group III (chronic renal disease), and group IV (diabetes with chronic renal disease).Two dorsal flaps were elevated. Flaps on left side of all groups received 0.5 cc of SVF, while same amount of plasma-buffered saline (PBS) was injected into right side. On postoperative day 7, flaps were harvested for macroscopic, histopathologic and biochemical assessments. Areas of flap survival were measured macroscopically. Blood level of vascular endothelial growth factor (VEGF) was measured after injection of SVF. Results Macroscopically, SVF has significantly improved flap viability (p < 0.05). Flap viability percentage was lower in DM and CRD groups when compared with healthy control group. In respect of new capillary formation, there was a statistically significant difference between SVF injected flaps and PBS injected sides (p < 0.05). Similarly, VEGF levels were higher in all study groups and there was a significant difference in comparison to control group (p < 0.05). Conclusions The study showed that injection of SVF increased flap viability via endothelial differentiation and neovascularization. In vivo function of stem cells might be impaired due to uremia and diabetes-related microenviromental changes.Item A Unique Case of solated Thoracic Spinal Rosai-Dorfman Disease Related to IgG4(2023) Terzi, Aysen; Sercan, Cigdem; Sahinturk, Fikret; 36226609Rosai-Dorfman disease (RDD) is a rare benign histiocytosis usually characterized by massive cervical lymphadenopathy and systemic manifestations. Extranodal, especially spinal involvement, is extremely rare. Our case was deemed worthy of presentation because it was the first reported isolated case of spinal RDD related to IgG4 and mimicked meningioma clinically and radiologically. A case with an intradural extramedullary mass causing neurological compression findings in the thoracic spinal region and radiologically mimicking meningioma is presented. In the histomorphological examination of the resection material, polymorphonuclear leukocytes in the dura, histiocytes showing emperipolesis, an increase in collagenized fibrous connective tissue, and intense lymphoplasmacytic cell infiltration accompanied by obliterative phlebitis were observed. Immunohistochemically, the histiocytic cells were found to be S-100 protein, CD68, and CD163 positive and CD1a and langerin negative, and more than half of the plasma cells were immunoglobulin-G4 (IgG4) positive. Although rare, RDD or IgG4-related meningeal disease should be considered in the differential diagnosis of dural-based spinal masses that radiologically suggest meningioma. The pathologist should be aware that these two histopathological entities may coexist. To our knowledge, this is the first case of "isolated spinal RDD related to IgG4" reported in the literature.Item The Effect of Adipose Stromal Vascular Fraction on the Viability of Transverse Rectus Abdominis Myocutaneous Flap after Abdominoplasty: An Experimental Study(2021) Coskun, Erhan; Ozkan, Burak; Terzi, Aysen; Ozer, Eda Ozturan; Uysal, Cagri Ahmet; Borman, Huseyin; Ertas, Nilgun Markal; 0000-0002-1225-1320; F-7546-2013Introduction: A prior abdominoplasty is considered as an absolute contraindication to transverse rectus abdominis musculocutaneous (TRAM) flap surgery. The aim of this study is to investigate the effect of nonexpanded adipose stromal vascular fraction (ASVF) on the viability of TRAM flap after abdominoplasty. Materials and Methods: Thirty-five male Sprague Dawley rats were divided into five groups. Reverse abdominoplasty model was used in all groups except Group 1. TRAM flap was performed 2 weeks after abdominoplasty in Groups 2 and 4 and 4 weeks after in Groups 3 and 5. ASVF cells were injected during abdominoplasty in Groups 4 and 5. The viable flap area (VFA) percentage and newly formed perforators were assessed. Capillary density and fibrosis gradient and plasma vascular endothelial growth factor (VEGF) levels were measured. Results: The mean VFA to total flap area was measured as 82.90% +/- 7.59%, 3.31% +/- 3.29%, 9.40% +/- 6.18%, 31.92% +/- 9.29%, and 64.98% +/- 10.95% in Group 1, Group 2, Group 3, Group 4, and Group 5, respectively (P < 0.05). The number of newly formed musculocutaneous perforating arteries was 0.29 +/- 0.49, 1.14 +/- 0.69, and 2 +/- 0.82 for Groups 3, 4, and 5, respectively (P < 0.05). The mean capillary density was 6.86 +/- 0.50, 0.67 +/- 0.13, 2.79 +/- 0.53, 3.71 +/- 0.47, and 7.01 +/- 0.70 in Groups 1, 2, 3, 4, and 5, respectively (P < 0.05). There was a statistically significant increase between the baseline VEGF values and the second VEGF values in Groups 4 and 5. Conclusions: The study showed that local injection of ASVF increases the viability of TRAM flap after abdominoplasty.Item The Promising Effects of Bmp2 Transfected Mesenchymal Stem Cells on Human Osteosarcoma(2021) Sari, Ahmet Sinan; Demircay, Emre; Ozturk, Ahmet; Terzi, Aysen; Karaoz, Erdal; 0000-0002-5429-1929; 0000-0003-1274-4288; 34377054; AAL-2368-2021Selective targeting of transfected mesenchymal stem cells (MSCs) carrying specific antioncogenes to the tumor was suggested as a treatment option. Bone morphogenetic protein-2 (BMP2) was shown to inhibit the proliferation and aggressiveness of osteosarcoma (OS) cells. Here, we aimed to assess the homing efficiency of intraperitoneally administered hMSCs transfected with BMP2 to the tumoral site and their effects on OS using an orthotopic xenograft murine model. Orthotopic xenograft murine model of OS in six-week-old female NOD/SCID mice using 143B cells was established. hMSCs transfected with BMP2 (BMP2(+)hMSC) were used. In vivo experiments performed on four groups of mice that received no treatment, or intraperitoneally administered BMP2, hMSCs, and BMP2(+)hMSCs. Histopathological and immunohistochemical studies were used to evaluate the pathological identification and to assess the dimensions and necrotic foci of the tumor, the features of lung metastases, and immunostaining against p27, Ki-67, and caspase-3 antibodies. The osteogenic differentiation markers BMP2, BMP4, COL1A1, OPN, OCN and PF4 evaluated using RT-PCR. The tumor dimensions in the hMSCs group were significantly higher than those of the remaining groups (p < 0.01). The number of metastatic foci in the BMP2(+)hMSCs group was significantly lower than those of the other groups (p < 0.01). The current results showed that the intraperitoneal route could be efficiently used for targeting hMSCs to the tumoral tissues for effective BMP2 delivery. In this study, the effects of BMP2 transfected hMSCs on human OS and metastasis were promising for achieving osteogenic differentiation and reduced metastatic process.Item Treatment of Full-Thickness Cartilage Defects with Pedunculated and Free Synovial Grafts: A Comparative Study in an Animal Model(2020) Haberal, Bahtiyar; Sahin, Orcun; Terzi, Aysen; Simsek, Ekin Kaya; Mahmuti, Ates; Tuncay, Ismail Cengiz; 0000-0002-6035-6258; 0000-0001-5856-8895; 0000-0002-1668-6997; 32850038; AAF-4032-2021; AAF-3988-2021; W-9080-2019Aims and Objectives The purpose of this study was to compare the potential effects of pedunculated and free synovial grafts in the repair of full-thickness articular cartilage defects on an animal model with histological and immunohistochemical analysis. Materials and Methods A comparative study in an animal model was performed with 24 rabbits, divided into two groups. Full-thickness cartilage defects were created bilaterally on the knees of all rabbits. Pedunculated and free synovial grafts were applied to the right knees of Group 1 and Group 2, respectively. Left knees were left as the control group. Six rabbits from each group were randomly selected for euthanasia 4 and 8 weeks postoperatively. All samples were examined histologically with a cartilage scoring system. For immunohistochemical analysis, the degree of collagen 2 staining was determined using a staging system. All data were statistically compared between the study groups with Student's t-test or Mann-Whitney U-test. The correlations between categorical variables were analyzed with Fisher's exact test and Chi-square test. Results In Group 1, the mean defect size had significantly decreased at 8 weeks postsurgery. It was also significantly smaller than that of Group 2. Both pedunculated and free synovial grafts had significantly better histological and immunohistochemical outcomes compared with the controls. Contrastingly, the results of comparison between the study groups (Group 1 vs. 2) at the 4th and 8th week were not statistically significant with regard to histological scores and immunohistochemical staining. Conclusion Synovial tissue, whether pedunculated or free, provided much better cartilage recovery compared with the control. It can be used as a mesenchymal stem cell (MSC) source, and synovium-derived MSCs have the chondrogenic potential for the in vivo treatment of full-thickness cartilage defects.Item The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease(2020) Eyuboglu, Atilla Adnan; Arpaci, Enver; Albayati, Abbas; Uysal, Ahmet Cagri; Terzi, Aysen; Bozalioglu, Sema; Turnaoglu, Hale; Balcik, Cenk; Ozkan, Burak; Ertas, Nilgun Markal; 0000-0002-0781-0036; 0000-0003-2806-3006; 0000-0001-6236-0050; 32784349; AAK-8242-2021; AAC-3344-2021; AAJ-2949-2021Background Chronic kidney disease (CKD) impairs osteoblast/osteoclast balance and damages bone structure with diminished mineralization and results in bone restoration disorders. In this study, we investigate the effects of adipose-derived stromal vascular fraction and platelet-rich plasma (PRP) on bone healing model in rats with CKD. Methods Sprague-Dawley rats were separated into 4 groups. All groups except group I (healthy control) had CKD surgery using 5/6 nephrectomy model. All groups had intramedullary pin fixation after receiving bone fracture using drilling tools. Group II rats were used as control group for CKD. Group III rats received PRP treatment on fracture site. Group IV rats received PRP and stromal vascular fraction treatment on fracture site. Weight loss and blood samples were followed at the time of kidney surgery, third, sixth, and 12th weeks. Bone healing and callus formations were compared, biomechanically, radiologically, histopathologically, and immunohistochemically. Osteoblastic transformation of stem cells was assessed with DiI staining. Results Negative effects of CKD on bone healing were reduced by increasing mechanical, histological, radiological, and biochemical properties of the bone with stromal vascular fraction and PRP treatments. Although thickness of callus tissue delayed bone healing process, it also enhanced biomechanical features and bone tissue organization. Conclusions Platelet-rich plasma and adipose-derived stromal vascular fraction treatments were effective for bone healing in animal model, which can be promising for clinical trials.Item COMPARISON OF PURE ANTIBODY-MEDIATED REJECTION WITH MIXED CELLULAR AND ANTIBODY-MEDIATED REJECTION IN REGARDS TO THE PATHOLOGICAL FEATURES, DEVELOPMENT OF CARDIAC ALLOGRAFT VASCULOPATHY (CAV) AND CARDIOVASCULAR MORTALITY (CVM) IN HEART TRANSPLANT PATIENTS(2020) Ozdemir, B. Handan; Terzi, Aysen; Ayva, Sebnem; Sade, L. Elif; Basturk, Bilkay; Sezgin, AtillaItem COMPARISON OF PURE ANTIBODY-MEDIATED REJECTION (AMR) WITH MIXED CELLULAR AND AMR IN REGARDS TO THE DEVELOPMENT OF CARDIAC ALLOGRAFT VASCULOPATHY (CAV) AND CARDIOVASCULAR MORTALITY (CVM) IN HEART TRANSPLANT PATIENTS(2019) Ozdemir, B. Handan; Ayva, Sebnem; Terzi, Aysen; Sade, L. Elif; Basturk, Bilkay; Sezgin, AtillaItem The effect of static magnetic field on partial thickness burn wound healing(2019) Soy, Ebru H. Ayvazoglu; Aydin, Huseyin Onur; Terzi, Aysen; Ekici, Yahya; Moray, GokhanAim: During the past decades, there has been a substantial and growing evidence that static magnetic fields (SMF) and pulsed electromagnetic fields (EMF) can both produce satisfying therapeutic effects on various disorders. There have been researches about the efficacy of SMF in wound healing. However, there is no any investigation about the efficacy of SMF in burn wound healing. In this experimental study, we aimed to investigate the efficacy of SMF in partial thickness burn wound in rats. Material and Method: Forty male Sprague-Dawley male rats were used in the experiment. The modification of Sawada's bum model was applied. In Group1 magnets, in Group2 non-magnetic bars were placed vertically to the burn wound. In Group3 magnets, in Group4 non-magnetic bars were placed horizontally to the burn wound. The wound heating is evaluated by gross wound healing rate and histopathological examination. Results: In study groups, wound healing was faster, granulation with fibroblast migration was earlier, collagen production and neovascularisation were more than control groups. We found no relationship between wound healing and vector direction. Discussion: According to these results static magnetic field can be used in clinic to promote the healing in patients with burns. Especially, in cases that have no chance for grafting, to decrease complications and increase the survival, the static magnetic field can be an alternative.