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search.filters.applied.f.rights: search.filters.rights.info:eu-repo/semantics/closedAccessSubject: search.filters.subject.Antioxidant

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    The Protective Effect of Prophylactic Ozone Administration Against Retinal Ischemia-Reperfusion Injury
    (2017) Kal, Ali; Kal, Oznur; Akillioglu, Ishak; Celik, Esin; Yilmaz, Mustafa; Gonul, Saban; Solmaz, Merve; Onal, Ozkan; https://orcid.org/0000-0001-7544-5790; https://orcid.org/0000-0002-7751-4961; 27028056; AAJ-4936-2021; AAJ-7586-2021
    Introduction: Retinal ischemia-reperfusion (IR) injury is associated with many ocular diseases. Retinal IR injury leads to the death of retinal ganglion cells (RGCs), loss of retinal function and ultimately vision loss. The aim of this study was to show the protective effects of prophylactic ozone administration against retinal IR injury.Materials and methods: A sham group (S) (n=7) was administered physiological saline (PS) intraperitoneally (i.p.) for 7 d. An ischemia reperfusion (IR) group (n=7) was subjected to retinal ischemia followed by reperfusion for 2h. An ozone group (O) (n=7) was administered 1mg/kg of ozone i.p. for 7 d. In the ozone+IR (O+IR) group (n=7), 1mg/kg of ozone was administered i.p. for 7 d before the IR procedure and at 8 d, the IR injury was created (as in IR group). The rats were anesthetized after second hour of reperfusion and their intracardiac blood was drawn completely and they were sacrificed. Blood samples were sent to a laboratory for analysis of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total oxidant score (TOS) and total antioxidant capacity (TAC). The degree of retinal injury was evaluated according to changes in retinal cells and necrotic and apoptotic cells using the TUNEL method. Data were evaluated statistically with the Kruskal-Wallis test.Results: The number of RGCs and the inner retinal thickness were significantly decreased after ischemia, and treatment with ozone significantly inhibited retinal ischemic injury. In the IR group, the degree of retinal injury was found to be the highest. In the O+IR group, retinal injury was found to be decreased in comparison to the IR group. In the ozone group without retinal IR injury, the retinal injury score was the lowest. The differences in the antioxidant parameters SOD, GSH-Px and TAC were increased in the ozone group and the lowest in the IR group. The oxidant parameters MDA and TOS were found to be the highest in the IR group and decreased in the ozone group.Discussion: IR injury is also positively correlated with the degree of early apoptosis. This study demonstrated that ozone can attenuate subsequent ischemic damage in the rat retina through triggering the increase of the antioxidant capacity.
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    Food plant with antioxidant, tyrosinase inhibitory and antimelanoma activity: Prunus mahaleb L
    (2022) Guven, Zuhal Bayrakceken; Dogan, Zeynep; Saracoglu, Iclal; Picot, Laurent; Nagatsu, Akito; Basaran, A. Ahmet
    Prunus mahaleb L. seeds are used as spice and folk remedies in many countries. The aim of this study was bioactivity guided isolation and characterization phytoconstituents of P. mahaleb which have been studied very limited so far. The antioxidant capacities of seed and kernel (endocarp) extracts were studied by DPPH, nitric oxide (NO), superoxide (SO), TEAC, CUPRAC methods. The antiproliferative activity were investigated for the first time against B16F10, A2058, HeLa and L929 cell lines using MTT assay. Gallic acid and ursolic acid which have strong antioxidant and cell growth inhibitory effect, were isolated from the most active fractions. From the inactive fractions, quercetin-3-O(2-O,6-O-alpha-dirhamnopyranosyl beta-glucopyranoside), kaempferol-3-O(2-O,6-O alpha-dirhamnopyranosyl beta-glucopyranoside) and beta-sitosterol-3-O-beta-glucopyranoside were isolated. Our study is the first record for the isolation of ursolic acid from all parts of P. mahaleb, gallic acid from P. mahaleb kernels, two flavonol triglycosides from the Prunus species and their analysis by advanced NMR spectroscopic methods. Ursolic acid (IC50 : 170.2 mu M) and gallic acid (IC50 : 10.5 mu M) showed strong tyrosinase inhibition, gallic acid being 5.4 times more effective than standard compound. Kernel extracts have strong antioxidant activity, showed high growth inhibitory activity with dose-dependent manner on melanoma cells but no growth inhibitory on healthy L929 and HeLa cancer cells. The high antioxidant capacity of kernel, its selective growth inhibition of B16F10 and A2058 melanoma cells and its strong tyrosinase inhibitory compounds suggest that it could be a new, non-toxic and inexpensive resource for melanoma treatment and cosmetic industry.
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    Assessment of the Physicochemical and Antioxidant Profile of Dried Goji Berries
    (2021) Agagunduz, Duygu; Koseler-Beyaz, Esra; Duman, Sinem; 0000-0003-0010-0012; AAS-9583-2020
    This study aimed to determine the physicochemical properties and antioxidant capacity of the dry goji berry fruit. Sun-dried goji berry fruits (Lycium barbarum) harvested in Manisa-Turkey and sold in the local market were collected. Total phenolic content (TPC) was determined by the Folin-Ciocalteu method, and the ferric reducing ability of plasma (FRAP) assay was utilized for antioxidant activity. 2,6 dichlorophenolindophenol spectrophotometric method was used in the ascorbic acid analysis. Mineral contents and the percentage contribution to the recommended daily allowance (RDA) of dry fruits were determined. TPC values of samples were 207.2 +/- 1.51 mg GAE/100 g and their antioxidant activities were found to be 32.6 +/- 1.82 mu mol TE/g. The fruit samples of 100 gram included 31.0 +/- 1.62 mg of ascorbic acid. Mineral contents and the percentage contribution to the RDA of 100 grams of dry goji berry fruits were as follows: calcium:49.0 mg (5.0%), phosphorus:370.0 mg (67.2%), sodium:1.32 mg (94.2%), potassium: 193.0 mg (>4000%), magnesium:120.0 mg (36.9%), iron:0.04 mg (0.3%), copper: 0.01 mg (0.7%) and manganese:0.008 mg (0.26%), The results of the present study suggest that goji berry cultivated in not only Asia but also Turkey certainly deserve further investigation because it contains a significant amount of vitamin C and some essential minerals, and with its phenolic content/antioxidant capacity even if it is traditionally sun-dried.