Fakülteler / Faculties
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Item Effect of Acrylamide Treatment on Arginase Activities and Nitric Oxide Levels in Rat Liver and Kidney(2014) Ozturan-Ozer, Eda; Ucar, Gulberk; Helvacioglu, Fatma; Akaydin-Aldemir, Derya; Turkoglu, Suna; https://orcid.org/0000-0001-6543-4043; https://orcid.org/0000-0002-6026-0045; https://orcid.org/0000-0003-4805-1918; AAK-3000-2021; AAH-8887-2021; AAJ-2243-2021Aim: To evaluate the effects of acrylamide treatment on the activities of rat liver, kidney arginase activities and nitric oxide levels considering the possible induction of oxidative stress. Materials and methods: Serum aminotransferase activities, blood-urea nitrogen (BUN) and creatinine concentrations and tissue malondialdehyde (MDA), reduced glutathione (GSH), total nitrite concentrations and arginase activities were evaluated in groups. Histopathological analysis was performed. Results: Acrylamide treatment did not modulate liver and kidney serum markers. Hepatic MDA, GSH concentrations did not change whereas they were elevated in kidney tissues of high dose treated group (p<0.05). Arginase activity in grain liver tissue decreased (p<0.0001), but specific activity did not alter. Total nitrite concentrations increased in high dose treated group (p<0.05). In kidney, high dose of acrylamide treatment elevated activity and specific activity of arginase (p<0.05). No alteration was detected in total nitrite levels. Ultrastructural alterations were detected in epithelial cells of proximal tubules in kidney sections of the rats treated with high dose of aculamide. Conclusion: Liver seems to protect itself against acrylamide toxicity whereas, kidney can be considered as a probable target tissue for acrylamide-induced oxidative stress.Item Impact of Rho-Kinase Inhibitor Hydroxyfasudil in Protamine Sulphate Induced Cystitis Rat Bladder(2015) Akin, Yigit; Bozkurt, Aliseydi; Erol, Huseyin S.; Halici, Mesut; Celebi, Fikret; Kapakin, Kubra A. T.; Gulmez, Hakan; Ates, Mutlu; Coban, Abdulkadir; Nuhoglu, Baris; 0000-0001-5467-3743; 26663691; Y-1659-2019ObjectivesThe objective of the present study was to evaluate anti-inflammatory effects of hydroxyfasudil in a protamine sulfate (PS) induced cystitis rat model. Additionally, we investigated prevention of bladder overactivity (BO), and tissue damage in these experiments. MethodsAnimals were divided into four groups. In Groups 1 and 2, chemical induced cystitis model was created by administrating intravesical PS with PE50 catheter by the transurethral route. In Group 1, Rho-kinase inhibitor hydroxyfasudil was administered intaperitoneally, and in Group 2, subjects were administered a corresponding volume of saline in the same way. In Group 3, vehicle was administered intravesically and hydroxyfasudil was administrated intraperitoneally. Group 4 was a control Group, and the vehicle was administered intravesically and intraperitoneally. Micturition frequencies were recorded. Biochemical analyses were performed for oxidative stress, and pathological evaluations were investigated. In vitro contractions of bladder tissue strips were measured in tissue-bath. ResultsThere were significantly lower Lipid peroxidase levels and higher levels of Glutathione in Group 1 than Group 2 (P=0.016, P=0.001, respectively). There was generally more inflammation in Group 2 than the other groups as determined by microscopy. There were significantly higher frequencies of micturition, lower volume, and mean voided maximum urine output after PS administration in Groups 1 and 2. In vitro contraction responses of bladder strips to potassium chloride and acetylcholine were statistically higher in Group 2 than Groups 1 and 3. ConclusionsSignificant reduction of inflammation by affecting the anti-oxidant defense systems was provided by hydroxyfasudil. Decreased in vitro responses to contractions of bladder smooth muscle strips were obtained. Hydroxyfasudil may be a potential new therapeutic option for inflammation and BO, in rat bladder.Item Superoxide Dismutase and Catalase Genotypes in Pediatric Migraine Patients(2015) Saygi, Semra; Erol, Ilknur; Alehan, Fusun; Yalcin, Yaprak Yilmaz; Kubat, Gozde; Atac, Atac, Fatma Belgin; 0000-0002-3530-0463; 0000-0001-6868-2165; 0000-0002-9337-9106; 0000-0002-8522-5078; 25818327; AAK-4825-2021; ABG-9966-2020; ABB-4078-2020; AAB-1203-2021This study compared superoxide dismutase (SOD) and catalase (CAT) alleles in 97 consecutive children and adolescents with migraine to 96 healthy children and adolescents. Isolated genomic DNA was used as a template for SOD1 (35 A/C), SOD2 16 C/T, and CAT2 [(-262 C/T) and (-21 A/T)] allele genotyping. The SOD2 16 C/T genotype and C allele frequency differed significantly between controls and migraine (P = .047; P = .038). CAT -21 AA genotype and A allele frequency were significantly higher in both migraine with aura patients (P = .013; P = .004) and migraine without aura patients (P = .003; P = .001) compared to controls. To our knowledge, this is the first demonstration of differences in SOD and CAT genotypes between pediatric migraine patients and age-matched controls. Further studies on the functional implications of these genetic variants on neural antioxidant capacity and the use of antioxidant modulators for migraine treatment are warranted.Item Comparison of Ischemia Modified Albumin Levels with Total Oxidant, Total Antioxidant Status, Oxidative Stress Index in Carbon Monoxide Poisoning(2014) Durukan, Polat; Koyuncu, Murat; Salt, Omer; Kavalci, Cemil; Ozkan, Seda; Muhtaroglu, Sebahattin; Kavalci, Gulsum; Ozdemir, Caglar; Duzgun, Ali; Ikizceli, Ibrahim; https://orcid.org/0000-0003-2529-2946; AGG-1308-2022Aim: The most common cause of death in CO poisoning is ventricular arrhythmias due to tissue hypoxia. In this study we aimed to investigate the relationship between severity of poisoning and Total Oxidant Status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) and also change in the levels of ischemia modified albumin (IMA) and neutrophil gelatinase-associated lipocalin (NGAL) over time in the patients with CO poisoning. Material and methods: This study was performed at Erciyes University Faculty of Medicine, Department of Emergency Medicine. Fifty patients between the ages of 18-65 who were diagnosed CO poisoning in the emergency department were included in the study. As a control group 30 adult individuals with no history of any disease were included in the study. Ischemia modified album, NGAL, OSI, TOS and TAS levels were studied. Mann-Whitney U test was using to compare of control and patient group. The Wilcoxon test was used to compare the change in TAS, TOS, OSI, IMA, NGAL, COHb and lactate. p<0.05 was considered as statistically significant. Results: When the 0th hour levels of Lactate, TOS, OSI, and IMA and TAS of the patient group were compared to the control group, there was a significant difference between these groups (p <0.05). There was no significant difference in terms of the NGAL level (p> 0.05). When 0th, 3rd, 6th, 12 and 24th hrs TAS, TOS, OSI, IMA, NGAL and lactate levels compared with each other, there was no difference between them (p>0.05). Conclusion: The levels of IMA, TOS, TAS and OSI were detected high in CO poisoning, but it is not meaningful in evaluating the effectiveness of treatment.Item Relationship Between Vitamin B12, Homocysteine and Oxidative Stress in Juvenile Idiopathic Arthritis(2016) Bicer, Nihan Cakir; Aksoydan, Emine; Zeybek, Cigdem Aktuglu; Barut, Kenan; Kasapcopur, OzgurJuvenile idiopathic arthritis (JIA), which is one of the rheumatic diseases, is a systemic inflammatory disease characterized by chronic and erosive synovitis that involves peripheral joints. In patients who had been diagnosed with JIA, increasing proinflammatory cytokines, metabolic abnormalities associated with systemic inflammation, may provoke vascular endothelial damage which can cause atherosclerosis. Homocysteine is another metabolite among the factors causing endothelial dysfunction. Homocysteine is an intermediate metabolite which is formed during the conversion of methionine to cysteine and high levels of homocysteine in blood can lead to vascular damage. Dietary folate and vitamin B12 deficiency can cause an increase in blood homocysteine levels. Vitamin B12 is essential for the transfer of methyl group and cell division in humans, but it is also important for the proliferation, maturation and regeneration of the nerve cells. In addition, "functional vitamin B12 deficiency" in which blood vitamin B12 level is in the normal range and without severe clinical symptoms like anemia has also been reported. Studies have showed that vitamin B12 deficiency can lead to oxidative stress without causing significant increase in homocysteine levels by its effects on cytokines, growth factors, nitric oxide metabolism, antioxidant enzymes and producing reactive oxygen species.Item Obesity and Oxidative Stress in Patients with Different Periodontal Status: A Case-Control Study(2017) Atabay, V. E.; Lutfioglu, M.; Avci, B.; Sakallioglu, E. E.; Aydogdu, A.; https://orcid.org/0000-0002-1738-371X; 26932579; ABA-1100-2020Background and ObjectiveObesity has become an important global health concern as obesity-associated adiposity is supposedly related to systemic immunologic and inflammatory alterations. The aim of this study was to evaluate the effects of obesity on periodontally healthy and diseased tissue according to the changes in malondialdehyde (MDA), protein carbonyl (PC) and total antioxidant capacity (TAOC) levels in gingival crevicular fluid as biomarkers of oxidative stress (OS). Material and MethodsThe study sample comprised systemically healthy normal-weight (n = 45) and obese (n = 48) adults. Obesity was diagnosed according to body mass index, waist circumference and waist/hip ratio. Periodontal status was evaluated according to plaque index, gingival index, bleeding on probing, probing depth and clinical attachment level. Participants were distributed among six groups according to obesity and periodontal status, as follows: normal weight+periodontally healthy (NH); normal weight+gingivitis (NG); normal weight+generalized chronic periodontitis (NCP); obese+periodontally healthy (OH); obese+gingivitis (OG); and obese+generalized chronic periodontitis (OCP). MDA, PC and TAOC levels were measured using ELISA. ResultsThe MDA and PC levels in gingival crevicular fluid varied among groups, as follows: NCP > NG > NH (p < 0.01) and OCP > OG > OH (p < 0.01). Conversely, the levels of TAOC in gingival crevicular fluid varied as follows: NCP < NG < NH (p < 0.01) and OCP < OG < OH (p < 0.01). Paired comparisons conducted according to periodontal status showed MDA and PC levels to be higher, and TAOC levels to be lower, in the OCP group than in the NCP group, in the OG group than in the NG group and in the OH group than in the NH group. However, only the differences between the OCP and NCP groups were significant (p < 0.01). In both obese and normal-weight individuals, clinical assessments showed significant, positive correlations with MDA and PC levels and negative correlations with TAOC levels (p < 0.01). ConclusionObesity may influence periodontal tissue destruction and disease severity by increasing the level of oxidative stress in the presence of periodontal disease.Item Protective Effects Of Alpha-Lipoic Acid On Bleomycin-Induced Skin Fibrosis Through The Repression Of NADPH Oxidase 4 And TGF-Beta 1/Smad3 Signaling Pathways(2022) Kocak, Ayse; Ural, Cemre; Harmanci, Duygu; Oktan, Mehmet Asi; Afagh, Aysan; Sarioglu, Sulen; Yilmaz, Osman; Birlik, Merih; Akdogan, Gul Guner; Cavdar, Zahide; https://orcid.org/0000-0002-9322-5844; 35187969; ABD-1329-2021The aim of this study was to determine the protective effects of alpha-lipoic acid (ALA), which is known as a powerful antioxidant, and the possible related molecular mechanisms that mediate its favorable action on skin fibrosis in the bleomycin (BLM)-induced scleroderma (SSc) model in mice. The experimental design was established with four groups of eight mice: Control, ALA (100 mg/kg), BLM (5 mu g/kg), and BLM + ALA group. BLM was administered via subcutaneous (sc) once a day while ALA was injected intraperitoneally (ip) twice a week for 21 days. Histopathological and biochemical analyses showed that ALA significantly reduced BLM-induced dermal thickness, inflammation score, and mRNA expression of tumor necrosis factor-alpha (TNF-alpha) in the skin. Besides, the mRNA expressions of the subunits of NADPH oxidase, which are Nox4 and p22phox, were found to be significantly induced in the BLM group. However, ALA significantly reduced their mRNA expression, which were in parallel to its decreasing effect on serum total oxidant status (TOS) level. Moreover, it was found that ALA downregulated the mRNA expressions of alpha-smooth muscle actin (alpha-SMA), collagen type I and fibronectin in the skin tissue of the BLM group. Additionally, it was shown that ALA reduced significantly the TGF-beta 1 and p-Smad3 protein expressions in the BLM + ALA group. On the other hand, ALA did not exhibit any significant effect on the p38 mitogen-activated kinase (MAPK) activation induced by BLM. All these findings point out that ALA may be a promising treatment for the attenuation of skin fibrosis in SSc patients.Item The Effects of Polymer Coating of Gold Nanoparticles on Oxidative Stress and DNA Damage(2020) Sen, Gamze Tilbe; Ozkemahli, Gizem; Shahbazi, Reza; Erkekoglu, Pinar; Ulubayram, Kezban; Kocer-Gumusel, Belma; 0000-0002-9421-6069; 32483993Gold nanoparticles (AuNPs) have been widely used in many biological and biomedical applications. In this regard, their surface modification is of paramount importance in order to increase their cellular uptake, delivery capability, and optimize their distribution inside the body. The aim of this study was to examine the effects of AuNPs on cytotoxicity, oxidant/antioxidant parameters, and DNA damage in HepG2 cells and investigate the potential toxic effects of different surface modifications such as polyethylene glycol (PEG) and polyethyleneimine (PEI; molecular weights of 2,000 (low molecular weight [LMW]) and 25,000 (high molecular weight [HMW]). The study groups were determined as AuNPs, PEG-coated AuNPs (AuNPs/PEG), low-molecular weight polyethyleneimine-coated gold nanoparticles (AuNPs/PEI LMW), and high-molecular weight polyethyleneimine-coated gold nanoparticles (AuNPs/PEI HMW). After incubating HepG2 cells with different concentrations of nanoparticles for 24 hours, half maximal inhibitory concentrations (the concentration that kills 50% of the cells) were determined as 166.77, 257.73, and 198.44 mu g/mL for AuNPs, AuNPs/PEG, and AuNPs/PEI LMW groups, respectively. Later, inhibitory concentration 30 (IC30, the concentration that kills 30% of the cells) doses were calculated, and further experiments were performed on cells that were exposed to IC30 doses. Although intracellular reactive oxygen species levels significantly increased in all nanoparticles, AuNPs as well as AuNPs/PEG did not cause any changes in oxidant/antioxidant parameters. However, AuNPs/PEI HMW particularly induced oxidative stress as evidence of alterations in lipid peroxidation and protein oxidation. These results suggest that at IC30 doses, AuNPs do not affect oxidative stress and DNA damage significantly. Polyethylene glycol coating does not have an impact on toxicity, however PEI coating (particularly HMW) can induce oxidative stress.Item The effect of nutrition theraphy on oxidative stress, inflammation, glycemic control in type 2 diabetes patients(2019) Colak, Gozde Aritici; Kiziltan, GulAims: Type 2 diabetes mellitus is a metabolism disease which is seen frequently among adult population in Turkey. The aim of this study was to determine the medical nutrition theraphy effect on oxidative stress, inflammation, glycemic control in type 2 diabetes patients. Methods: An interventional study was carried on 35 type 2 diabetes ages between 20-65 years old at the Department of Endocrinology of Baskent University Istanbul Hospital in 2015. In 3 month period a personal nutrition theraphy was applied. Biochemical parameters, anthropometric measurements and body analysis were also determined. The three day food consumption and biochemical parameters were requested at the beginning and at the end of the study. Results: When the impact of the blood values of the new medical nutrition theraphy which the patients practiced during the first visit and the follow up visit were compared; it was seen that there was a significant decrease on fasting plasma glucose, fasting insulin, HbA1c, CRP, TG and MDA values (p<0.05). The mean diabetic age of the patients was 7.63 +/- 6.22 years. When diabetic age of the patients was increased, there was a positive correlation between the fasting plasma glucose and HbA1c values (p<0.05). Conclusions: Type 2 diabetes patients were evaluated 3 month of medical nutrition theraphy and it was seen that the personal medical nutritional theraphy contributed to providing glisemic control and decreasing oxidative stress and inflammation.