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Author: search.filters.author.Helvacioglu, FatmaSubject: search.filters.subject.immunohistochemistry

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    Does Ginkgo biloba Protect Developing Testes from Chronic Hypobaric Hypoxia?
    (2017) Gul, Elif Ensari; Kaplanoglu, Gulnur Take; Helvacioglu, Fatma; Kaplanoglu, Iskender; Seymen, Cemile Merve; 0000-0002-6026-0045; 0000-0002-8945-3801; AAH-8887-2021; AAS-5415-2021
    OBJECTIVE: To evaluate the potential protective effects of Ginkgo biloba on developing testes exposed to chronic hypobaric hypoxia during their prenatal life. STUDY DESIGN: Twelve pregnant Wistar albino rats on the fifth gestational day were placed in hypoxic chambers for the upcoming 15 days. Six pregnant female rats were kept under normal atmospheric conditions during the pregnancy as a control group. The study groups were as follows: Control, Hypoxia, and Hypoxia + Ginkgo biloba. Ginkgo biloba was administered for each designated postnatal sacrifice day. For the Hypoxia + Ginkgo biloba group, after birth, 100 mg/kg of Ginkgo biloba extract was administrated orally to the newborn male rats. Testes tissues were sampled on postnatal days 7, 14, and 21 from each group, and PCNA, TUNEL, and TEM examinations were performed. RESULTS: PCNA immunoreactivity was decreased and TUNEL positive cell number was increased in the hypoxic group. TEM examination revealed degenerative changes in hypoxic group testes tissue. Hypoxia changed the cell cycle in spermatogenic series by reducing proliferation and increasing apoptosis. Spermatogenic cell degeneration and high Leydig cell activity were determined in the hypoxia group by TEM examinations. CONCLUSION: We believe that Ginkgo biloba has protective effects on testes tissue, especially in long-term use.
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    Prostaglandin F receptor expression in intrauterine tissues of pregnant rats
    (2014) Anadol, Elvan; Kanca, Halit; Yar, Atiye Seda; Helvacioglu, Fatma; Menevse, Sevda; Calguner, Engin; Erdogan, Deniz
    In this investigation, we studied the expression and localization of rat prostaglandin F (FP) receptor in uterine tissues of rats on gestational Days 10, 15, 18, 20, 21, 21.5 and postpartal Days 1 and 3 using Western blotting analysis, real-time PCR, and immunohistochemistry. A high level of immunoreactivity was observed on gestational Days 20, 21, and 21.5 with the most significant signals found on Day 20. FP receptor protein was expressed starting on gestational Day 15, and a fluctuating unsteady increase was observed until delivery. Uterine FP receptor mRNA levels were low between Days 10 and 18 of gestation (p < 0.05). The transcript level increased significantly on Day 20 and peaked on Day 21.5 just before labor (p < 0.05). There was a positive correlation between FP receptor mRNA expression and serum estradiol levels (rs = 0.78; p < 0.01) along with serum estradiol/progesterone ratios (rs = 0.79; p < 0.01). In summary, we observed an increase FP receptor expression in rat uterus with advancing gestation, a marked elevation of expression at term, and a concominant decrease during the postpartum period. These findings indicate a role for uterine FP receptors in the mediation of uterine contractility at term.