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Author: search.filters.author.Gurgen, Seren GulsenHas files: Yes

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    Protective Effect of Spirulina on Cisplatin-Induced Ototoxicity: A Functional and Histopathological Study
    (2022) Tahir, Emel; Buyuklu, Adnan Fuat; Ocal, Fatma Ceyda Akin; Gurgen, Seren Gulsen; Sarsmaz, Hayrunnisa Yesil
    Objective: The purpose of this study was to evaluate the protective effect of an antioxidant and anti-inflammatory agent, "spirulina," against cisplatin-induced ototoxicity in rats. Methods: Twenty-eight adult Sprague-Dawley rats were divided into 4 groups. Before drug administration, distortion product otoacoustic emission and auditory brainstem response tests were performed. Group 1 (n =7) received 1 mg of intraperitoneal saline. Group 2 (n=7) received a single dose of intraperitoneal cisplatin at 15 mg/kg/day. Group 3 (n=7) received oral spirulina at 1000 mg/kg/day for 10 days. Group 4 (n=7) received a single i.p. dose of cisplatin at 15 mg/kg/day, followed by 10 days of oral spirulina at 1000 mg/kg/day. The final distortion product otoacoustic emission and auditory brainstem response measurements were provided 10 days after the initial drug administration. Cochleas were removed, the histochemical examination was performed by caspase-3, caspase-9, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling methods. Results: Initially, there were no significant differences in distortion product otoacoustic emission and auditory brainstem response measurements between groups. Following cisplatin treatment, the mean difference in signal to noise ratio values was lower in the cisplatin + spirulina group compared to the cisplatin-only group. The increase in auditory brainstem response thresholds was more significant in the cisplatin-only group than in the cisplatin + spirulina group. Posttreatment auditory brainstem response latencies were prolonged in cisplatin and cisplatin + spirulina groups; however, a significant difference was obtained between these 2 groups. The cisplatin + spirulina group had a lower density of apoptotic cells than the cisplatin-only group. Conclusion: Spirulina has no adverse effects on cochlear functions and may provide some protection against cisplatin-induced ototoxicity.
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    Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity
    (2018) Goktas, Guleser; Seyhan, Sermin; Saribas, Gulistan Sanem; Akcay, Neslihan Coskun; Gurgen, Seren Gulsen; Akyol, Seda Nur; Hirfanoglu, Ibrahim Murat; Erdogan, Deniz; Ozogul, Candan
    Objective: The aim of the study was to investigate the prophylactic effects of acetyl L-carnitine against to uterus induced by cisplatin. Methods: Twenty-four female Wistar albino rats were divided into four groups: group I (control) was administered with saline; group II was administered with acetyl L-carnitine; group III was administered with cisplatin; group IV was pretreated with acetyl L-carnitine before cisplatin intraperitoneal injection. After 72h of cisplatin injection uterine tissue was removed. Histological and immunohistochemical investigations were performed, respectively. Results: We found that the number of TUNEL and caspases positive cells were increased in the endometrial epithelium, subepithelial connective tissue, endometrial glands and stroma in group III compare to the other groups. Furthermore inflammation and edema were observed in uterus of rats in group III. Conclusion: We can concluded that pretreatment of acetyl L-carnitine administration has protective effect on histological alteration of uterus caused by cisplatin.