Eczacılık Fakültesi / Faculty of Pharmacy

Permanent URI for this collectionhttps://hdl.handle.net/11727/5700

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Author: search.filters.author.Enes, Duygusearch.filters.applied.f.language: search.filters.language.en_US

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    Comparative Plant Metabolomics of Momordica charantia Seeds and Fruits
    (Başkent Üniversitesi Eczacılık Fakültesi, 2024-03-30) Enes, Duygu; Fidan, Bilge Basak; Basaran, Arif Ahmet; Celebier, Mustafa
    Momordica charantia L., Cucurbitaceae, known mainly as karela, bitter gourd or bitter melon, and balsam pear, is used for antihyperglycemic, antibacterial, antiviral, antitumor, immunomodulation, antioxidant, antidiabetic, anthelmintic, antimutagenic, antiulcer, antilipolytic, antifertility, hepatoprotective, anticancer, and anti-inflammatory and wound healing. This study aimed to elucidate the differences in the metabolites of 70% methanol extracts of M. charantia seeds and fruit using untargeted metabolomics. Liquid chromatography coupled to quadrupole time-of-flight mass spectrometry-based analysis of the extracts for both seed and fruit was performed using a C-18 column. Differences were observed in seed and fruit extracts, which were visualized using principal component analysis plots. (R)-Salsolinol, pantetheine, coumarin, tryptamine, lysophospholipidPC(O-18:0), glucosylceramide, pyroglutamic acid, and presqualene diphosphate in the seed and fruit of M. charantia were detected in different levels. The amount of lysophospholipidPC(O-18:0) (lysoPC(O-18:0)) and glucosylceramide is high in the fruit, while the amount of (R)-salsolinol, pantetheine, coumarin, tryptamine, presqualene diphosphate, and pyroglutamic acid is high in the seed. These primary untargeted metabolomic results revealed that the different pharmacological effects may be related to the variable amounts of some specific metabolites in seeds and fruits.
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    Development And Uv-Vis Spectrophotometric Analysis Of An Ease-Of-Use Pediatric Oral Solution Of Dexamethasone For Personalized Therapies
    (JOURNAL OF RESEARCH IN PHARMACY, 2024-10-02) Enes, Duygu; Fidan, Bilge Basak; Kaplan, Ozan; Dogan, Aysegul; Altinoz, Sacide; Celebier, Mustafa; Kaynak, Mustafa Sinan
    The usage of dexamethasone for pediatric applications is a well-known issue. In the present study, we developed an oral dexamethasone solution formulation especially aimed for dose-dependent personalized therapies and having excipients known as not harmful to be safely used in pediatrics. The aim of this study was to prepare an easy-of-use pediatric oral solution of dexamethasone and develop an UV/VIS Spectrophotometric method for the evaluation of the stability and quality control of the developed formulation. The primary source of dexamethasone for preparation of the oral pediatric solution was the dexamethasone one-time injectable solutions. This allowed the formulation to be easily prepared in basic laboratory conditions. Dexamethasone content and stability of the formulation were ensured by quantification using the developed UV/VIS Spectrophotometric method validated based on ICH Q2 (R1) guidelines. Simple, fast, reliable, and validated spectrophotometric analysis of dexamethasone was carried out at 269 nm wavelength and the method was linear in a range of 1.00 to 50.00 mu g mL-1.The developed formulation was stable at 4 degrees C at least for three weeks when protected from daylight. The other stability conditions (ambient temperature and -20 degrees C) were also evaluated for the assays. Although the methodology used in this study contains simple processes which can be easily adapted to basic laboratory conditions, the results were satisfactory to prepare an ease-of-use pediatric oral solution of dexamethasonefor personalized medicine. The validated UV/VIS Spectrophotometric method was selective for the formulation and easily applied for the quality control and stability studies of the samples. Such formulations could be helpful for health professionals in managing real-life corticosteroid treatment application problems especially for pediatrics in hospital pharmacy.