Mühendislik Fakültesi / Faculty of Engineering

Permanent URI for this collectionhttps://hdl.handle.net/11727/1401

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    Accelerating The Environmental Biodegradation Of Poly-3-Hydroxybutyrate (Phb) Via Plasma Surface Treatment
    (BIORESOURCE TECHNOLOGY REPORTS, 2024-09-02) Akdogan, Ebru; Sirin, Hasret Tolga; Sahal, Gulcan; Deniz, Zulkuf; Kaya, Ayberk; Serdaroglu, Dilek Cokeliler
    The surface of poly-3-hydroxybutyrate (PHB) was modified using a low-pressure plasma system with air as the process gas to accelerate its biodegradation rate in soil. The water contact angle of PHB was reduced from 98 degrees to 57 degrees after plasma treatment, rendering the surface hydrophilic and also induced an increase in the surface free energy. Etching on the surface was observed after the plasma treatment without a significant change in the surface crystallinity. AFM imaging showed that the plasma treatment increased the surface roughness by about 10 folds and created diverse surface structures. The soil burial test showed an approximately 1.5-fold increase in the biodegradation rate for the plasma-treated sample. Initial microbial attachment and biofilm formation were higher on the modified surface. This study demonstrated that the surface morphology created by plasma treatment promoted initial colonization and subsequent biofilm formation on the PHB surface, facilitating and accelerating its biodegradation in soil.
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    Tumour-Specific Hybrid Nanoparticles In Therapy Of Breast Cancer
    (JOURNAL OF MICROENCAPSULATION, 2024-01-02) Kilicay, E.; Erdal, E.; Elci, P.; Hazer, B.; Denkbas, E. B.
    In this study, salicylic acid (SA) dopped into poly(3-hydroxybutyrate) (PHB) and prepared nanoparticles (NPs) to increase encapsulation efficiency, anti-cancer activity of caffeic acid (Caff), and folic acid (FA) for breast cancer treatment. NPs were prepared by solvent evaporation method and characterised by FTIR, DSC, SEM, and entrapment-loading efficiencies. The mean diameter, polydispersity index (PDI), and zeta potential (ZP) were evaluated by dynamic light scattering (DLS). In vitro release and stability studies were done via eppendorf method. The cytotoxicity, cell dead and internalisation of NPs were shown by MTT, fluorescein and confocal microscopy. The diameter and ZP of NPs were 172 +/- 7 nm and -29 +/- 0.38 mV. The entrapment efficiencies of 5 and 10 Caff NPs were 79 +/- 0.23% and 70 +/- 0.42%. NPs showed good stability within 30 d and sustained release over 25 d. FA-5Caff NPs showed 37 +/- 0.3% viability on MCF-7. FA-Caff NPs were identified as promising carrier system for breast cancer therapy.