Browsing by Author "Kazanci, Ferah"

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Cytogenetic Profile and Phenotypical and Reproductive Complaints in 39 Patients with Turner Syndrome: A Single Center Experience
(2023) Kazanci, Ferah; Celik, Zerrin; https://orcid.org/0000-0001-9158-220X; A-1229-2015
Background/Aim: Turner syndrome (TS) is a genetic failure that influence phenotypic girls who have full or incomplete monosomy of X chromosome with a variety of clinical signs. The purpose of this study was to estimate TS cases based on their cytogenetic findings and clinical implications. Material and methods:Thirty-nine cases diagnosed with TS were retrospectively analyzed between November 2006 and December 2019. These patients were identified among 505 people who had their karyotypes analyzed for different reasons, including primary amenorrhea (PA), premature ovarian insufficiency (POI), TS phenotype, and uterine agenesis (UA). Karyotype analysis was carried out using Giemsa staining in accordance with the standard method on peripheral blood and fluorescence in situ hybridization (FISH) was used when necessary. Results: The median age of TS cases were 15 years (ranging from 4 to 32). The distribution of reasons for admission was as follows: 61.5% TS phenotype, 25.6% PA, 10.3% POI, and 2.6% UA with horseshoe kidney. The frequency of cytogenetic finding was 38.5% pure monosomy X and 61.5 % mosaic [30.7% monosomy X with structural rearrangements, 18% with X chromosomal structural abnormalities, 7.7% with X aneuploidy and 5.1% with Y chromosomal structural abnormalities]. The most accepted reason for both pure and mosaic TS group was TS phenotype. Conclusion: TS develops when one sex chromosome is wholly or incompletely removed as well as structurally altered. Phenotype, fertility, and life quality may differ according to the variability of cytogenetic findings. Comprehensive cytogenetic analysis is required for the patients for medical follow-up and genetic counselling.
The prevalence and distribution of human papillomavirus in 4267 Turkish women with or without cervical lesions: A hospital-based study
(2022) Altay-Kocak, Aylin; Kazanci, Ferah; Dogu-Tok, Canan; Onan, Anil; Erdem, Ozlem; Ozkan, Secil; Bozdayi, Gulendam; 0000-0002-0451-0142; 35676203; AAI-8012-2021
In the present study, it was aimed to screen the genotypes of human papillomavirus (HPV) retrospectively in women with gynecological symptoms who were admitted to a tertiary care university hospital in Ankara, Turkey. A total of 4267 cervical swab samples of women aged 18-79 years were sent to Medical Virology Laboratory from January 2017 to November 2020. Nucleic acid extraction and amplification of samples were done by an automated system. The test can detect 14 high-risk HPV (HR-HPV) types in a single analysis that performs a real-time polymerase chain reaction, by providing individual results on the highest-risk genotypes HPV 16 and HPV 18 and pooled results on other high-risk genotypes (OHR-HPV) (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68). HPV DNA positivity was detected in 14.2% (605/4267) of the samples. HPV type 16 and type 18 were detected in 2.4% and 0.7% of the samples, respectively. OHR-HPV types were found in 8.8% of the samples. Of the 1.9% and 0.4% samples had mixed types with type 16+ OHR-HPV and type 18+ OHR-HPV, respectively. The results of this study presented the rates of HR-HPV genotypes of a university hospital in Ankara, over a 4-year period. It was observed that the positivity rate of type 18 is decreasing and some OHR-HPV types are increasing. HPV vaccination is not in the national immunization program in Turkey yet, however, HPV vaccines are available and the vaccination rates for women are increasing.
Should We Accept the HPV Type 66 into a Probable High-Risk Group? The Prevalence, Clinical and Histopathological Evaluation of HPV Type 66 in Gazi University, Ankara
(2021) Kazanci, Ferah; Kocak, Aylin Altay; Colak, Meryem; Erdem, Ozlem; Onan, M. Anil; Bozdayi, Gulendam; 0000-0002-0451-0142; AAI-8012-2021
Introduction: The prevalence of infection by different genotypes of human papillomavirus (HPV) varies among different geographic areas. The objective of the study is to determine the prevalence and distribution of HPV66 genotype among women with normal or abnormal Pap smear tests. Methods: This retrospective study was conducted in a tertiary care university hospital between January 2017 and February 2018, in central Anatolia of Turkey. This study included 288 women, 66 (%22.9) of whom had HPV DNA positive. HPV DNA screening was done by an automatized system using real time PCR method (Cobas 4800 System, Roche Diagnostics Ltd, Switzerland) and this method distinguishes types 16 and 18, while the other 12 oncogene types are reported as high-risk HPV (HR-HPV: 31,33,35,39,45,51,52,56,58,59,66,68). For the genotyping of other oncogene types, a commercial real time PCR method (NLM Genotypes 14 Real-TM Quant, NLM Diagnostic, Italy) was used. Results: The most common identified HPV types were HPV16 (%6.3), HPV 56 (%3.8), HPV 18(%3.1), HPV 66 (%3.1), HPV 51 (%2.8), HPV 52(%2.1). HPV type 66 which has admitted recently other-subtypes with their unclear oncogenicity is the third most identified type in our study. In our study 9 (%3.1) women had type 66 and 2 (%0.7) of whom had abnormal Pap smear results. One patient with syphilis whose pap smear test results was ASC-H/HSIL was evaluated by colposcopic examination and LEEP (Loop Electrosurgical Excision Procedure) and ECC (Endocervical Curettage) were performed. The result of histopatological report was benign. The other patient whose Pap smear test result was LSIL evaluated by colposcopic examination and found no pathological finding. Conclusion: The frequency of HPV 66 infection was found to be higher in our study compared to previous reports. In 2 patients out of 9 cases (% 2.4) who were detected HPV 66 had normal pap test results.