Browsing by Author "Guven, Aysel"

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EFFECT OF GREEN TEA (CAMELLIA SINENSIS L.) AND PARSLEY (PETROSELINUM CRISPUM) DIETS AGAINST CARBON TETRACHLORIDE HEPATOTOXICITY IN ALBINO MICE
(2019) Guven, Aysel; Nur, Gokhan; Deveci, Haci Ahmet
In this study, it has been tried to be searched the effects of green tea (Camellia sinensis L.) and parsley (Petroselinum crispum) that is known their antioxidant features on levels of MDA, GSH and activities of GSH-Px, GST, CAT on mice liver tissue. In the study, total 32 Swiss albino mice has been used that 8 of them are control and 24 of them are practise. Group I: animals were put on a normal diet and sham-treated with 2 ml/kg distilled water through oral gavage, daily for 8 weeks; this group of animals served as the control. Group II: animals were put on a normal diet and treated with 1.5 ml/kg b.w CCl4 dissolved in 1.5 ml distilled water through oral gavage. Group III: animals were put on a normal diet and treated with 1.5 ml/kg b.w CCl4 + 500 mg/kg b.w green tea through oral gavage. Group IV: animals were put on a normal diet and treated with and for CCl4 + 0,464 g/kg parsley oral gavage daily for 8 weeks. As a results, by increasing antioxidant enzymes levels on groups given green tea and parsley to control group (p<0,05), but in MDA levels has been seen a significant decreasing as statistical (p<0.01). While both Green tea and Parsley were found to have a protective effect against CCl4 induced damage, Parsley was more protective. Histopathological analysis revealed that liver tissue appeared normal in control on the other hand, there were degeneration, congestion, cellular infiltration, and necrotic areas in the group administered with CCl4. Even though frequency of lesions decreased, similar lesions were observed in the group with 1,5 ml/kg b.w CCl4. Consequently, it was determined that while CCl4 administration increased oxidative stress, green tea and parsley administration had a protective potential increasing antioxidant capacity. Natural antioxidant substances found in parsley and green tea can be considered as the best green chemical substances to cope with oxidative stress without damaging the nature and the living.
Evaluation of Radioactivity Levels and Radiological Hazards of Some Endemic Plants Used as Medicine in Ankara, Turkey
(2023) Guven, Aysel; Cengiz, Gulcin Bilgici; Caglar, Ilyas; Ates, Simge; 37536028
In this study, natural radioactivity levels (Ra-226, Th-232, and (4) K) of some medicinal plant samples with known anti-oxidative properties, which are frequently consumed by animals and humans, were obtained from Ankara province and its surroundings (Mamak, Kizilcahamam, Beypazari, Kahramankazan, and Polatli districts) were determined using a thallium-doped sodium iodide NaI(Tl) gamma spectrometry. By using the determined natural radioactivity concentrations in the collected plant samples, the number of radiological doses that people could be exposed by consuming these plants was calculated. As a result of the study, Ra-226, Th-232, and (4) K radioactivity concentration ranges of the plant samples were found be 14.69 +/- 1.27-59.08 +/- 3.12 Bq kg(-1), 1.78 +/- 0.04-50.05 +/- 2.76 Bq kg(-1) and 207.24 +/- 34.09-826.13 +/- 25.40 Bq kg(-1), respectively. The highest Ra-226, Th-232, and (4) K activity concentrations were measured in Astragalus densifolius subsp. ayashensis (Kahramankazan), Astragalus kochakii (Kahramankazan) and Rumex patientia (Patience Dock) (Kahramankazan) plants, respectively. The lowest Ra-226, Th-232 and,(4) K activity concentration plants were determined respectively as Rumex patientia (Mamak), Lavandula angustifolia (Kizilcahamam), and Astragalus acikirensis (Polatli). The establishment and routine repetition of environmental radioactivity monitoring programs in each region are important for human and animal health, and the results of this study gain importance for Ankara and its surroundings in terms of environmental health.
HEV seroprevalence in blood donors in Turkey by two commercial total anti-HEV Ab ELISA kits
(2019) Yasar, Osman; Karatayli, Ersin; Cengiz, Guniz; Kizilpinar, Mehtap; Yurdcu, Esra; Albayrak, Rabia; Guven, Aysel; Arslan, Onder; Karahan, Ceren; Otlu, Baris; Guducuoglu, Huseyin; Gokahmetoglu, Selma; Berk, Elife; Gitmisoglu, Ozlem; Sertoz, Ruchan; Yurdaydin, Cihan; Bozdayi, A. Mithat; Karatayli, Senem Ceren; 31403185
Previous hepatitis E virus (HEV) seroprevalence studies in Turkey have shown high variabilities, leading to conflicting results. We aimed to re-evaluate HEV seroprevalence among blood donors in Turkey using the Wantai (Beijing, China) and the Dia.Pro (Milan, Italy) total anti-HEV antibody (Ab) enzyme-linked immunosorbent assay (ELISA) kits and compare their performances and to investigate the presence of HEV RNA in blood donors. Serum total anti-HEV antibodies were determined in a total of 2011 volunteer blood donor samples collected from different regions of Turkey (807 from Ankara, 243 from Kayseri, 284 from Izmir, 200 from Malatya, 200 from Kahramanmaras, and 277 from Van). HEV RNA was evaluated by a real-time polymerase chain reaction in a total of 272 anti-HEV seropositive samples. The country-wide HEV seroprevalence was calculated as 11.5% (Dia.Pro) and 12.2% (Wantai) with seropositivity rates of 12.0%-12.5% in Ankara, 7.4%-8.2% in Kayseri, 14.5%-15.5% in Malatya, 8.1%-8.8% in Izmir, 15.0%-16.0% in Kahramanmaras, and 12.6%-13.4% in Van by Dia.Pro and Wantai kits, respectively. The lowest detectable Ab concentrations were 0.16 and 0.14 units/mL WHO, for the Dia.Pro and the Wantai assays, respectively, showing no significant difference between assays. HEV RNA was not detected in any of the anti-HEV seropositive samples. Compared with previous studies, HEV was shown to have a higher overall seroprevalence in Turkey. Despite its limitation, the current study represents the most comprehensive HEV seroprevalence study in Turkey performed with two different commercial ELISA assays with high sensitivities so far. Further investigation is required to determine HEV genotypes in Turkey.
HEV Seroprevalence in Blood Donors in Turkey: Comparison of Two Commercial Anti-HEV Total Ab ELISA Kit
(2019) Yasar, Osman; Cengiz, Guniz; Karatayli, Ersin; Kizilpinar, Mehtap; Yurdcu, Esra; Albayrak, Rabia; Arslan, Onder; Karahan, Zeynep Ceren; Otlu, Baris; Guven, Aysel; Guducuoglu, Huseyin; Gokahmetoglu, Selma; Berk, Elife; Gitmisoglu, Ozlem; Sertoz, Ruchan; Yurdaydin, Cihan; Mithat, Bozdayi; Karatayli, Senem Ceren