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Item Impact of radial extracorporeal shock wave therapy in post-laminectomy epidural fibrosis in a rat model(2021) Haberal, Bahtiyar; Simsek, Ekin Kaya; Akpinar, Koray; Simsek, Duygu Turkbey; Sahinturk, Fikret; https://orcid.org/0000-0002-1668-6997; https://orcid.org/0000-0003-3438-1633; https://orcid.org/0000-0002-0471-3177; 33463432; W-9080-2019; AAV-8821-2021; AAI-7972-2021Objectives: This study aims to investigate the effect of radial extracorporeal shock wave therapy (rESWT) treatment in the prevention of epidural fibrosis after laminectomy in rats. Materials and methods: Eighteen 16-month-old male Sprague-Dawley rats weighing 300 g were used in this experimental study between November 2019 and February 2020. The rats were randomly divided into two groups as the control group (L3-L4 total laminectomy without any treatment) and the study group (L3-L4 total laminectomy plus rESWT). The rats were sacrificed at the postoperative sixth week and the lumbar spine was excised en bloc, fixed, and decalcified. Sections were stained with hematoxylin-eosin to evaluate epidural fibrosis, acute inflammation, chronic inflammation, and vascular proliferation. Results: The median value and standard deviations were obtained based on histological examinations. Accordingly, epidural fibrosis decreased significantly in the study group compared to the control group. There was no statistically significant difference between the groups in terms of acute and chronic inflammation response and vascular proliferation. Conclusion: The rESWT application immediately after surgery is effective in preventing epidural fibrosis after laminectomy in rats.Item Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage-dependent calcium channels(2020) Efe, Oguzhan E.; Lux, K. Michael; Emre Aydingoz, Selda; Tuncer, Meral; 0000-0002-3243-7843; 0000-0003-3360-5092; 0000-0001-7823-7620; 32830389; W-7908-2019; AAD-9901-2021; ABA-4291-2020Aim We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur-containing foods such as garlic on rat uterine activity. Methods Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague-Dawley rats (250-300 g) in estrus stage of estrous cycle were studied in isolated organ baths containing Krebs-Henseleit solution. Results Diallyl sulfide (10(-8)-10(-6) M) caused a concentration-dependent relaxation on KCl (60 mM)-induced contractions and inhibited spontaneous peristaltic activity of uterine strips (P < 0.05). None of the following antagonists significantly changed the inhibitory effect of DAS on both KCl-precontracted uterine strips and spontaneous peristaltic activity of the uterus (P > 0.05): nitric oxide synthase inhibitor L-NAME (10(-4) M), hydrogen sulfide-producing enzymes cystation beta synthase and cystation gamma-lyase inhibitors, aminooxyacetic acid (10(-4) M) and propargylglycine (10(-3) M) and nonselective cyclooxygenase inhibitor indomethacin (10(-4) M). However, in calcium-free Krebs solution containing high KCl (30 mM), DAS significantly inhibited CaCl2(10(-5)-10(-2) M)-induced uterine contractions in a concentration-dependent manner (P < 0.05). Conclusion Diallyl sulfide has a relaxing effect on KCl-contracted rat uterus strips and an inhibitory effect on spontaneous uterine activity, possibly by decreasing the calcium influx into the cytoplasm of uterine smooth muscle cells.Item Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model(2020) Totuk, Ozgan Melike Gedar; Guzel, Sevket Ergun; Ekici, Husamettin; Kumandas, Ali; Aydingoz, Selda Emre; Yilmaz, Enis Cagatay; Kirdan, Taylan; Midi, Ahmet; 0000-0001-7823-7620; 33107963; ABA-4291-2020BACKGROUND: Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model. METHODS: Forty-eight Sprague Dawley rats (5-7 weeks old, 180-210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped. RESULTS: Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0 +/- 1.2 seconds and 8.0 +/- 1.3 seconds in heparinized and non-heparinized groups, respectively. CONCLUSION: AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.Item The effect of N-acetyl cysteine on biofilm layers in an experimental model of chronic otitis media(2020) Callioglu, Elif Ersoy; Bercin, Sami; Basdemir, Gulcin; Kiris, Muzaffer; Tatar, Ilkan; Tuzuner, Arzu; Oguzhan, Tolga; Muderris, Tuba; Sargon, Mustafa Fevzi; Korkmaz, Mehmet Hakan; 33558775Objective. The aim of this study was to investigate the efficacy of N-acetylcysteine (NAC) on biofilm layers and on the course of disease in chronic otitis media. Methods. Twenty-five rats that were induced with chronic otitis media (COM) were separated into three groups. In Group 1 (N = 18), 0.2% ciprofloxacin + 0.1% dexamethasone sodium phosphate + 0.5 mg/ml NAC solution was locally injected to the right ear of the rats; in Group 2, (N=18) 0.2% ciprofloxacin + 0.1% dexamethasone sodium phosphate was locally injected to the left ear of the rats. No treatment was applied to either ear of rats in Group 3 (N = 5). Histopathological and scanning electron microscope (SEM) evaluations were performed in all groups. Results. SEM revealed biofilm formation in all COM induced groups. No significant difference was seen between groups 1 and 2 in terms of suppuration levels, fibrosis, inner ear involvement, infection staging and biofilm formation (p > 0.05). Conclusions. In this study, while histopathological and SEM evaluation revealed no effect of 0.5 mg/ml NAC on the biofilm layer in COM-induced rats, further studies with NAC at different concentrations are still needed on different types of experimental animals.Item Prostaglandin F receptor expression in intrauterine tissues of pregnant rats(2014) Anadol, Elvan; Kanca, Halit; Yar, Atiye Seda; Helvacioglu, Fatma; Menevse, Sevda; Calguner, Engin; Erdogan, DenizIn this investigation, we studied the expression and localization of rat prostaglandin F (FP) receptor in uterine tissues of rats on gestational Days 10, 15, 18, 20, 21, 21.5 and postpartal Days 1 and 3 using Western blotting analysis, real-time PCR, and immunohistochemistry. A high level of immunoreactivity was observed on gestational Days 20, 21, and 21.5 with the most significant signals found on Day 20. FP receptor protein was expressed starting on gestational Day 15, and a fluctuating unsteady increase was observed until delivery. Uterine FP receptor mRNA levels were low between Days 10 and 18 of gestation (p < 0.05). The transcript level increased significantly on Day 20 and peaked on Day 21.5 just before labor (p < 0.05). There was a positive correlation between FP receptor mRNA expression and serum estradiol levels (rs = 0.78; p < 0.01) along with serum estradiol/progesterone ratios (rs = 0.79; p < 0.01). In summary, we observed an increase FP receptor expression in rat uterus with advancing gestation, a marked elevation of expression at term, and a concominant decrease during the postpartum period. These findings indicate a role for uterine FP receptors in the mediation of uterine contractility at term.Item Protective effects of growth hormone on bacterial translocation and intestinal damage in rats with partial intestinal obstruction(2014) Kaymakci, A.; Guven, S.; Ciftci, I.; Akillioglu, I.; Aktan, M.; Eker, H.H.; Sutcu, A.; Abasiyanik, A.; 25077360Objective: One of the reasons of bacterial translocation (BT) is the complete or partial intestinal obstructions (PIO) of the gastrointestinal system. In this study, we aimed to investigate the effects of recombinant human Growth Hormone (rhGH) on BT in rats with partial intestinal obstruction (PIO). Material and methods: The rats were randomly divided into the 4 groups: Group I: Sham-operated (SO) (n = 12), Group II control PIO (n = 12), Group III: PIO with rhGH treatment for 5 days (n = 12), Group IV: PIO with rhGH treatment 5 days before PIO and 5 days after PIO (a total of 10 days) (n = 12). In the groups III and IV, the effects of 5 and 10 days administered rhGH were examined. Results: The level of serum and of intestinal fluid IgA was significantly higher in the Group IV compared to the Group I, Group II and Group III. In the Group IV, the number of small intestinal goblet and colonic goblet cells, and the lengths of intestinal mucosal villi and crypt depths were statistically significantly higher than in Groups II and III. The rate of bacterial translocation was higher in the Group II: 100 % in MLNs, 41.6 % in blood culture and 50.8 % in the liver cultures, it was significantly higher compared to the other groups (p < 0.01). Conclusions: The study results demonstrated that administration of rhGH to the rats with PIO for at least 10 days decreased bacterial translocation (Fig. 3, Ref. 25). Text in PDF www.elis.sk.Item Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model(2014) Aydos, Tolga Resat; Basar, Mehmet Murad; Kul, Oguz; Atmaca, Hasan Tank; Uzunalioglu, Tuba; Kisa, Ucler; Efe, Oguzhan Ekin; 25539540Background/aim: To investigate the effect of ozone and/or taurine treatment comparatively on testicular damage due to ischemia/reperfusion (I/R) injury in an experimental torsion model in rats. Materials and methods: Adult Wistar rats with and without torsion/detorsion were used. In order to monitor the effect of ozone and/or taurine treatment on testicular damage due to I/R injury, following histopathological investigation apoptotic indexes were scored by TUNEL method. Moreover, tumor necrosis factor receptor 1 (TNFR1), caspase 3, caspase 8, endothelial nitric oxide synthase (eNOS), tumor necrosis factor alpha (TNF alpha), and cytochrome C immunostainings were performed and the levels of malondialdehyde, glutathione peroxidase, superoxide dismutase, catalase, total sulthydryl, and nitric oxide were determined in the testicular tissue. Results: Intraperitoneal ozone and/or taurine treatment prevented both histopathological damage and increase in the apoptotic index. Torsion did not exert an effect on the levels of TNFa and cytochrome C. Ozone and/or taurine treatment prevented increases in TNFR1, caspase 3, and caspase 8. The level of oxidative stress markers was unchanged. The increases in NO level and eNOS expression were prevented by ozone and/or taurine treatment in I/R groups. Conclusion: Using ozone therapy and/or taurine before reperfusion may be a solution for germ cell degeneration resulting from testicular torsion and related infertility.Item Evaluation of light-emitting diode photobiomodulation on bone healing of rat calvarial defects(2015) Deniz, Ediz; Arslan, Ahmet; Diker, Nurettin; Olgac, Vakur; Kilic, ErtugrulOne of the major problems in modern dentistry is the recovery of bone defects. The aim of this prospective experimental study was to evaluate the effect of light-emitting diode (LED) photobiomodulation therapy on bone healing of rat calvarial defects. Twenty-eight male Sprague Dawley rats were used for the study. Critical size defects with 5 mm diameter were made with a trephine bur used in a low-speed handpiece under continuous sterile saline irrigation on each side of the sagittal suture. All critical size defects on the right side were filled with corticocancellous bone graft material and all the defects on the left side were left empty. The animals were randomly divided into two groups of 14 rats each. Group I received LED therapy and Group II did not receive any therapy. OsseoPulse (R) LED device (Biolux Research Ltd.) 618 nm wavelength and 20 mW/cm(2) output power irradiation was started immediately after the surgery and was applied for 20 minutes at 24-h intervals for 7 and 14 days. In each group, seven rats were sacrificed on the 8th day and the remaining rats were sacrificed on the 15th day. Bone healing of the non-grafted side was statistically significant in Group I on both 8th day and 15th day; on the other hand, in the grafted side, enhanced bone healing was dominantly observed on the 15th day in Group I, compared to Group II, although the difference was not significant. Within the limits of this study, the findings suggested that LED therapy might have a favourable effect in the early phase of bone healing.