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Item Food plant with antioxidant, tyrosinase inhibitory and antimelanoma activity: Prunus mahaleb L(2022) Guven, Zuhal Bayrakceken; Dogan, Zeynep; Saracoglu, Iclal; Picot, Laurent; Nagatsu, Akito; Basaran, A. AhmetPrunus mahaleb L. seeds are used as spice and folk remedies in many countries. The aim of this study was bioactivity guided isolation and characterization phytoconstituents of P. mahaleb which have been studied very limited so far. The antioxidant capacities of seed and kernel (endocarp) extracts were studied by DPPH, nitric oxide (NO), superoxide (SO), TEAC, CUPRAC methods. The antiproliferative activity were investigated for the first time against B16F10, A2058, HeLa and L929 cell lines using MTT assay. Gallic acid and ursolic acid which have strong antioxidant and cell growth inhibitory effect, were isolated from the most active fractions. From the inactive fractions, quercetin-3-O(2-O,6-O-alpha-dirhamnopyranosyl beta-glucopyranoside), kaempferol-3-O(2-O,6-O alpha-dirhamnopyranosyl beta-glucopyranoside) and beta-sitosterol-3-O-beta-glucopyranoside were isolated. Our study is the first record for the isolation of ursolic acid from all parts of P. mahaleb, gallic acid from P. mahaleb kernels, two flavonol triglycosides from the Prunus species and their analysis by advanced NMR spectroscopic methods. Ursolic acid (IC50 : 170.2 mu M) and gallic acid (IC50 : 10.5 mu M) showed strong tyrosinase inhibition, gallic acid being 5.4 times more effective than standard compound. Kernel extracts have strong antioxidant activity, showed high growth inhibitory activity with dose-dependent manner on melanoma cells but no growth inhibitory on healthy L929 and HeLa cancer cells. The high antioxidant capacity of kernel, its selective growth inhibition of B16F10 and A2058 melanoma cells and its strong tyrosinase inhibitory compounds suggest that it could be a new, non-toxic and inexpensive resource for melanoma treatment and cosmetic industry.Item Assessment of the Physicochemical and Antioxidant Profile of Dried Goji Berries(2021) Agagunduz, Duygu; Koseler-Beyaz, Esra; Duman, Sinem; 0000-0003-0010-0012; AAS-9583-2020This study aimed to determine the physicochemical properties and antioxidant capacity of the dry goji berry fruit. Sun-dried goji berry fruits (Lycium barbarum) harvested in Manisa-Turkey and sold in the local market were collected. Total phenolic content (TPC) was determined by the Folin-Ciocalteu method, and the ferric reducing ability of plasma (FRAP) assay was utilized for antioxidant activity. 2,6 dichlorophenolindophenol spectrophotometric method was used in the ascorbic acid analysis. Mineral contents and the percentage contribution to the recommended daily allowance (RDA) of dry fruits were determined. TPC values of samples were 207.2 +/- 1.51 mg GAE/100 g and their antioxidant activities were found to be 32.6 +/- 1.82 mu mol TE/g. The fruit samples of 100 gram included 31.0 +/- 1.62 mg of ascorbic acid. Mineral contents and the percentage contribution to the RDA of 100 grams of dry goji berry fruits were as follows: calcium:49.0 mg (5.0%), phosphorus:370.0 mg (67.2%), sodium:1.32 mg (94.2%), potassium: 193.0 mg (>4000%), magnesium:120.0 mg (36.9%), iron:0.04 mg (0.3%), copper: 0.01 mg (0.7%) and manganese:0.008 mg (0.26%), The results of the present study suggest that goji berry cultivated in not only Asia but also Turkey certainly deserve further investigation because it contains a significant amount of vitamin C and some essential minerals, and with its phenolic content/antioxidant capacity even if it is traditionally sun-dried.