Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed
Permanent URI for this communityhttps://hdl.handle.net/11727/4806
Browse
5 results
Search Results
Item The prevalence and distribution of human papillomavirus in 4267 Turkish women with or without cervical lesions: A hospital-based study(2022) Altay-Kocak, Aylin; Kazanci, Ferah; Dogu-Tok, Canan; Onan, Anil; Erdem, Ozlem; Ozkan, Secil; Bozdayi, Gulendam; 0000-0002-0451-0142; 35676203; AAI-8012-2021In the present study, it was aimed to screen the genotypes of human papillomavirus (HPV) retrospectively in women with gynecological symptoms who were admitted to a tertiary care university hospital in Ankara, Turkey. A total of 4267 cervical swab samples of women aged 18-79 years were sent to Medical Virology Laboratory from January 2017 to November 2020. Nucleic acid extraction and amplification of samples were done by an automated system. The test can detect 14 high-risk HPV (HR-HPV) types in a single analysis that performs a real-time polymerase chain reaction, by providing individual results on the highest-risk genotypes HPV 16 and HPV 18 and pooled results on other high-risk genotypes (OHR-HPV) (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68). HPV DNA positivity was detected in 14.2% (605/4267) of the samples. HPV type 16 and type 18 were detected in 2.4% and 0.7% of the samples, respectively. OHR-HPV types were found in 8.8% of the samples. Of the 1.9% and 0.4% samples had mixed types with type 16+ OHR-HPV and type 18+ OHR-HPV, respectively. The results of this study presented the rates of HR-HPV genotypes of a university hospital in Ankara, over a 4-year period. It was observed that the positivity rate of type 18 is decreasing and some OHR-HPV types are increasing. HPV vaccination is not in the national immunization program in Turkey yet, however, HPV vaccines are available and the vaccination rates for women are increasing.Item Detection of HPV DNA in Esophageal Lesions: a Cross-Sectional Study(2020) Dinc, Bedia; Altay-Kocak, Aylin; Aydog, Gulden; Kuran, Sedef; Akoglu, Musa; Ozkan, Secil; Bozdayi, Gulendam; 32162873Background: Several studies have documented human papillomavirus (HPV) in extra-cervical tumors. We aimed to detect HPV type 16 and HPV other than type 16 (OT-16) DNA in esophageal papilloma and esophagus squamous cell carcinoma (ESCC) samples and to compare clinicopathological features of HPV positive and negative patients. Methods: Materials were obtained from a tertiary care public hospital and studied in an university hospital for this cross-sectional study. Seventy-six tissue samples (50 papilloma and 26 ESCC) were included. After deparaf-finization by xylene and DNA extraction by phenol chloroform-isoamyl-alcohol, 76 samples were studied with a G6PDH control kit. Forty-four papilloma and 21 ESCC samples with enough tissues were studied for HPV DNA. HPV OT-16 DNA and HPV type 16 were detected by real time-polymerase chain reaction. Results: Twelve (27.3%) and one (2.3%) of the papilloma samples were HPV type 16 and other than type 16 positive, respectively. Eleven (52.4%) and one (4.8%) of ESCC samples were HPV type 16 and mixed type positive, respectively. Conclusions: We suggest that HPV infection is common in esophageal papilloma and ESCC. Due to the wellknown association of HPV with premalignant and malignant conditions, follow-up of these patients accompanied by HPV should be implemented.Item Evaluation of 30 and 90 Day Hospital Readmission Rates in Severe COPD Exacerbations: Turkish Thoracic Society COPD Assembly(2020) Polatli, Mehmet; Baha, Ayse; Korkturk, Nurdan; Yapar, Dilek; Ozkan, Secil; Sen, Elif; Ciftci, Fatma; Turk, Burcu Oz; Kodalak, Sumeyye; Ulubay, Gaye; Serifoglu, Irem; Varol, Yelda; Mertoglu, Aydan; Cirak, Ali Kadri; Turan, Onur; Dursunoglu, Nese; Savurmus, Nilufer; Gurgun, Alev; Elmas, Funda; Coplu, Lutfi; Sertcelik, Umran; Yildiz, Reyhan; Ozmen, Ipek; Alpaydin, Aylin; Yesiloglu, Ebru Karacay; Celik, DenizItem Difficult to manage COPD Exacerbations: Risk factors (DIMECO Study). Turkish Thoracic Society COPD Assembly(2020) Baha, Ayse; Korkturk, Nurdan; Yapar, Dilek; Ozkan, Secil; Sen, Elif; Ciftci, Fatma; Ozturk, Burcu; Kodalak, Sumeyye; Ulubay, Gaye; Serifoglu, Irem; Varol, Yelda; Mertoglu, Aydan; Cirak, Ali Kadri; Turan, Onur; Dursunoglu, Nese; Savurmus, Nilufer; Gurgun, Alev; Elmas, Funda; Coplu, Lutfi; Sercelik, Umran; Yildiz, Reyhan; Ozmen, Ipek; Alpaydin, Aylin; Polatli, Mehmet; Yesiloglu, Ebru Karacay; Celik, DenizItem Is Human Papillomavirus and Helicobacter pylori Related in Gastric Lesions?(2019) Bozdayi, GUlendam; Dinc, Bedia; Avcikucuk, Havva; Turhan, Nesrin; Altay-Kocak, Aylin; Ozkan, Secil; Ozin, Yasemin; Bostanci, Birol; 31625359Background: Human papillomavirus (HPV), the causative agent of cervical cancer, is also suggested as a risk factor for gastric adenocarcinoma. Many infectious agents besides Helicobacter pylori have been associated with gastritis. The aim of this study was to investigate HPV DNA and genotyping HPV type 16 DNA in gastric adenocarcinoma and Helicobacter pylori gastritis cases. Methods: A hundred and six gastric adenocarcinoma and Helicobacter pylori gastritis samples and 26 controls were included. After deparaffinization by xylene, DNA extraction was performed by the phenol-chloroform-isoamyl alcohol method and 106 samples were studied with a G6PDH control kit (Eurogentec, Seraing, Belgium). Fifty-three adenocarcinoma and 43 Helicobacter pylori samples were thought to have enough tissue and were studied for HPV DNA. HPV types other than 16 and HPV type 16 DNA were detected by Real Time PCR using the L1 region. Amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV DNA and HPV 16 DNA specific probe in Light Cycler 2.0 (Roche Diagnostics, Germany) device. Results: Among gastric adenocarcinoma and Helicobacter pylori gastritis samples, 20/53 (38%) and 18/43 (41.8%) were HPV DNA positive, respectively. Five (19.2%) of 26 controls were HPV DNA positive. Conclusions: Our 38% positive result in the gastric carcinoma group is in concordance with previous reports. This is the first study revealing the HPV-H. pylori relationship in gastritis cases and we concluded that with regard to the nearly three-fold higher HPV DNA (41.8%) in gastritis cases compared to controls, Helicobacter pylori positive cases should also be evaluated in favor of HPV in the gastritis group.