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Author: search.filters.author.Kocak, Aylin AltaySubject: search.filters.subject.RT-PCR

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    Investigation of Group A Rotavirus G10, G12 Genotypes Emerging in Patients with Acute Gastroenteritis in a Tertiary Care Hospital
    (2021) Kahraman, Hande; Kocak, Aylin Altay; Albakkour, Katren; Muftah, Hager; Dalgic, Buket; Caglar, Kayhan; Ahmed, Kamruddin; Bozdayi, Gulendam; 34666655
    Rotaviruses are the most common cause of viral gastroenteritis with the highest mortality and morbidity rates in children aged 0-5 years. The aim of this study was to determine the frequency of rotavirus infection in patients whose stool samples were sent to microbiology laboratory to investigate the etiology of diarrhea, to investigate the rotavirus genotypes that are common in our region and G10, G12 genotypes that have recently become common in the world. Fecal samples of 476 patients aged between 0-92 years who applied between November 2016 and February 2018 were studied via immunochromatographic rapid test and enzyme-linked immunosorbent assay (ELISA) methods. ELISA positive samples were studied by nested reverse transcriptase chain reaction (RT-PCR) and genotyped by agarose gel electrophoresis. Rotavirus was found positive in 18.3% and 17% of stool samples by immunochromatographic test and ELISA, respectively. All ELISA positive samples were also detected as positive by RT-PCR. 18.5% of female patients and 15.7% of male patients were found to be positive and rotavirus positivity was not statistically significant between genders. The frequency of rotavirus in different age groups was 23.5% (6-12 years), 17.3% (13-24 months) and 16% (25-36 months). It was determined that rotavirus cases were most common in the spring. G1, G2, G3, G4, G9, G10, and G12 were detected in 37%, 7.4%, 16.1%, 6.2%, 9.9%, 2.5%, 26% of the samples, respectively. G12 was the most common genotype after G1. The most common G and P genotype combination was G1P[8] (17.2%). This was followed by G12P[8] (11.11%) and G3P[8] (11.11%). P[8] (53%) was found to be the dominant P genotype. In this study, it was observed that rotavirus, which is the cause of childhood diarrhea, can also be encountered in advanced ages and even new genotypes that infect humans worldwide may also be the causative agents. Therefore, we concluded that it is important to investigate new genotypes such as G10 and G12 in molecular epidemiological studies.
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    Investigation of the Rotavirus Genotypes Isolated From Patients With Acute Gastroenteritis and the Increase of G9 Type
    (2021) Kocak, Aylin Altay
    Introduction: Rotavirus (RV) is the most common cause of gastroenteritis in children and is one of the most common cause of mortality and morbidity in developing countries. The aim of this study was to determine the genotypes of RV rapid test antigen positive patients between 0-65 years old with acute gastroenteritis attended to a tertiary care hospital in Ankara. Materials and Methods: This study was conducted between January 2013 and April 2018 at Gazi University Faculty of Medicine, Department of Microbiology, Stool samples were collected from 87 (40 female, 47 male) patients aged between 0-65 years who had gastroenteritis were sent to microbiology laboratory. RV VP-7 amplification was performed using Beg9 and End9 primers and specific primers for G typing G1-G4 and G9. VP-4 amplification was performed using con-2 and con-3 primers. P types were determined by specific primers for P[4], P[6], P[8] and P[9]. Access Quick RT-PCR (Promega Corporation, Madison, WI) was used for VP4 and VP7 gene amplification, and PCR Mastermix (Promega, Madison, WI) was used for genotyping. Results: No statistically significant difference was found between the gender of patients with positivity of RV antigen. RV infection was most common in children aged 12-23 months. Antigen positivity was most common in winter and autumn. Genotypes G1 (25.80%), G2 (3.22%), G3 (4.30%), G4 (6.45%), G9 (60.21%) constituted G types. P4 (1.14%), P8 (93.10%), P6 (5.74%) constituted P types. The combination of G and P types was the most prevalent for G9P[8] (56.98%) and G1P[8] (22.58%). Conclusion: It has been observed that G9 and P[8] are common genotypes in cases with RV infection in Ankara as in the whole world. Similar to various studies in Turkey, genotype G9P[8] had the highest ratio in the present study. Therefore, these data should be considered in RV vaccine strategies.