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Item Xanthan-Gelatin And Xanthan-Gelatin-Keratin Wound Dressings For Local Delivery Of Vitamin C(2022) Demir, Gizem Cigdem; Erdemli, Ozge; Keskin, Dilek; Tezcaner, Aysen; https://orcid.org/0000-0001-8606-8863; 000780824900002; AAF-4496-2019Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings. This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC). Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycerol concentrations and characterized to determine the hydrogel composition. According to their weight ratios, xanthan, gelatin, and glycerol hydrogels are named. If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2. X1: GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity. The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release. Water vapor transmission of XGHs and KXGHs was between 3059.09 & PLUSMN; 126 and 4523 & PLUSMN; 133 g m(-2) d(-1); therefore, they can be suitable for granulating, low to moderate exudate wounds. XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds. VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts. All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission. In conclusion, our results suggest that VC-XGH and VC-KXGH can be candidates for temporary wound dressing materials for skin wounds.Item Gold nanocomposites for biomedical applications(2019) Akturk, Omer; Erdemli, Ozge; Tunali, Beste Cagdas; 0000-0001-8606-8863; AAF-4496-2019; AAG-3927-2019Item One-pot facile synthesis of silk sericin-capped gold nanoparticles by UVC radiation: Investigation of stability, biocompatibility, and antibacterial activity(2019) Akturk, Omer; Gok, Zehra Gun; Erdemli, Ozge; Yigitoglu, Mustafa; 0000-0001-8606-8863; 31393664; AAG-3927-2019; AAF-4496-2019Herein, an easy one-pot synthesis method for gold nanoparticles (AuNPs), involving only gold salt and sericin extracted from silkworm cocoon in the presence of ultraviolet C (UVC) radiation, was developed. Nanoparticle formation was confirmed by characteristic surface plasmon resonance peaks at 520-540 nm wavelengths, and the influence of silk sericin on enhancing the colloidal stability of AuNPs was confirmed. Transmission electron microscopy examination showed the average size (<10 nm) and size distribution decreased significantly with higher sericin concentration. No antibacterial activity was observed on Gram-positive Bacillus subtilis or Gram-negative Escherichia coli for sole AuNPs (0.065-0.26 mg/ml), but the conjugation of AuNPs with streptomycin antibiotic decreased significantly the required minimum inhibitory concentration doses, as also confirmed with agar plating, Scanning Electron Microscopy and Atomic Force Microscopy analyses. Furthermore, sericin-capped AuNPs showed high cell viabilities (>100%) and no sign of any detectable apoptosis or necrosis in 1-day incubation. Also, high real-time cell proliferation results of AuNPs competitive with positive control groups implied excellent in vitro biocompatibility. These results evidenced that sericin enhanced the colloidal stability of AuNPs and the biological activities of sericin-capped AuNPs reported here could render them suitable nanoscale vehicles for biomedical applications.