Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed
Permanent URI for this communityhttps://hdl.handle.net/11727/4806
Browse
2 results
Search Results
Item Effect Of Tacrolimus In The Inner Ear Of Rats(2022) Uysal, Fatmanur; Erbek, Selim S.; Erbek, Seyra; Culhaoglu, BeldePurpose: Tacrolimus, an immunosuppressive agent, is used especially after organ transplantation. It has been asserted that tacrolimus has protective effects on the auditory system in some studies while it has negative effects in other studies. The purpose of our study is to investigate the effect of tacrolimus on the inner ear of the rats. Materials and Methods: 20 healthy Sprague Downey male rats weighing 250-350 grams were included in our study. The first group of rats were given 1mg/kg tacrolimus (n:7), the second group of rats were given 0.1 mg/kg tacrolimus (n:7), and the third group (n:6) was the non-administered control group. The first measurements of all rats were taken with distortion-product otoacoustic emission before starting the experiment. Then, tacrolimus drug was administered by gavage method to the 1st and 2nd groups along 30 days. The last measurement was repeated on the 30th day. Results: According to the results of the first measurements, emission was obtained in all rats and the responses were found to have similar characteristics. Similarly, the difference between the signal noise rate values in the last measurements taken from the groups not show any statistical significance. Conclusions: Based on the distortion-product otoacoustic emission measurements, it can be said that Tacrolimus does not have ototoxic effects on the auditory system of rats considering the administered dosage and time.Item Protective effect of Nigella sativa oil on acoustic trauma induced hearing loss in rats(2017) Culhaoglu, Belde; Erbek, Selim S.; Erbek, Seyra; Hizal, Evren; 0000-0002-8453-6069; 0000-0003-4825-3499; 0000-0002-9699-6783; 28791082; AAJ-2445-2021; B-7604-2019; A-5853-2018Acoustic trauma is a common reason for hearing loss. Different agents are used to prevent the harmful effect of acoustic trauma on hearing. The aim of this study was to evaluate the potential preventive effect of Nigella sativa (black cumin) oil in acoustic trauma. Our experimental study was conducted with 20 Sprague Downey female rats (mean age, 12 months; mean weight 250 g). All of the procedures were held under general anesthesia. Following otoscopic examinations, baseline-hearing thresholds were obtained using auditory brainstem responses (ABR). To create acoustic trauma, the rats were then exposed to white band noise of 4 kHz with an intensity level of 107 dB in a soundproof testing room. On Day 1 following acoustic trauma, hearing threshold measurements were repeated. The rats were divided into two groups as the study group (n: 10) and the controls (n: 10). 2 mL/kg/day of Nigella sativa oil was given to the rats in the study group orally. On Day 4 following acoustic trauma, ABR measurements were repeated again. There was no difference between the baseline hearing thresholds of the rats before acoustic trauma (P>0.005). After the acoustic trauma, hearing thresholds were increased and there was no significant statistically difference between the hearing thresholds of the study and control groups (P=0.979). At the 4th day following acoustic trauma, hearing thresholds of the rats in control group were found to be higher than those in the study group (P=0.03). Our results suggest that Nigella sativa oil has a protective effect against acoustic trauma in early period. This finding should be supported with additional experimental and clinical studies, especially to determine the optimal dose, duration and frequency of potential Nigella sativa oil therapy.