Wos İndeksli Açık & Kapalı Erişimli Yayınlar

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Subject: search.filters.subject.ischemia reperfusion injury

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    The Healing Effects of The Topical Mesenchymal Stem Cell Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury
    (2021) Niyaz, Mehmet
    Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs.
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    Postconditioning Ozone Alleviates Ischemia-Reperfusion Injury and Enhances Flap Endurance in Rats
    (2020) Elsurer, Cagdas; Onal, Merih; Selimoglu, Nebil; Erdur, Omer; Yilmaz, Mustafa; Erdogan, Ender; Kal, Oznur; Celik, Jale Bengi; Onal, Ozkan; 0000-0002-7751-4961; 30339503; AAJ-7586-2021
    Introduction: Muscle-flap transferring is a routine approach utilized in reconstructive operations; however, flap morbidity is often a source of post-operative difficulty. Ischemia-Reperfusion Injury (IRI) is an important contributor to the viability of flaps after transferring. The goal of this research was for assess the probable useful impacts of ozone on flap survival in a rat muscle-flap design. Materials and Methods: We examined the effects of postconditioning ozone administration on viability of pedicled composite flaps. Twenty-eight Wistar rats were randomized into four groups: sham-operated (S), ischemia-reperfusion (IR), sham-operated + ozone (O), IR + ozone (IR + O), respectively. The animals were sacrificed on the eighth day. In a general histological evaluation, flap tissues were examined with a light microscope, and apoptotic cells were counted. The Apoptotic Index (AI) was then calculated. Flap-tissue samples were sent for analyses of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and protein carbonyl (PCO), and blood samples were sent for analyses of Total Oxidant Score (TOS), and Total Antioxidant Capacity (TAC). Data were evaluated statistically using the Kruskal-Wallis test. Results: The histomorphometric score was remarkably greater in O (p = .002). The AI was greater in IR (p = .002). The antioxidant parameters values as regards SOD, GSH-Px, CAT, and TAC were found to be greater in O (p < .005). The oxidant parameters values as regards MDA, PCO, TOS were found to be greater in IR (p < .005). Discussion: The current research indicates that ozone application can attenuate the muscle-flap injury brought about by IR through triggering the increase of the antioxidant capacity.
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    The effect of pregabalin on tourniquet-induced ischemia-reperfusion injury: a prospective randomized study
    (2019) Karaca, Omer; Pinar, Huseyin Ulas; Ozgur, Ahmet Fevzi; Kulaksizoglu, Sevsen; Dogan, Rafi; 0000-0003-0473-6763; 31655508; Q-2420-2015
    Background/aim: The aim of this study was to investigate the efficacy of pregabalin on ischemia-reperfusion injuries. Materials and methods: Fifty-four patients were randomly assigned into 2 groups. A 150-mg tablet of pregabalin was given the night before and then 1 h before the operation for patients in Group P (pregabalin group, n = 27). A placebo was given to patients in Group C (control group, n = 27) at the same times. After combined spinal-epidural anesthesia was performed, venous blood samples were taken before tourniquet inflation (t(1)), just before tourniquet deflation (t(2)), and 20 min after tourniquet deflation (t(3)) for the analysis of total antioxidant status (TAS), total oxidant status (TOS), catalase (CAT), and ischemia-modified albumin (IMA). Results: There was no significant difference in TAS levels between the groups for the t(3) period. However, the TAS in Group P was significantly higher in the t(3) period than the t(2) period (mean +/- SD, 0.46 +/- 0.1 vs. 0.38 +/- 0.2 mmol of Trolox equivalent/L, respectively; P < 0.05). The CAT level in the C period was significantly higher in Group P than Group C (mean +/- SD, 53.04 +/- 32.1 vs. 35.46 +/- 17.2 mu mol/formaldehyde, respectively; P < 0.05). In the t(3) period, the TOS was significantly lower in Group P than Group C (mean +/- SD, 11.97 +/- 5 vs. 18.29 +/- 9.9 pg/mL, respectively; P < 0.05). The TOS in Group P was significantly lower in the t(3) period than the t(2) period (mean +/- SD, 11.97 +/- 5 vs. 18.98 +/- 10.7 pg/mL, respectively; P < 0.0001). Conclusion: Pregabalin has no marked antioxidant activity, but it contributes to the antioxidant defense system of an organism.