Wos İndeksli Açık & Kapalı Erişimli Yayınlar

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    Is Human Papillomavirus and Helicobacter pylori Related in Gastric Lesions?
    (2019) Bozdayi, GUlendam; Dinc, Bedia; Avcikucuk, Havva; Turhan, Nesrin; Altay-Kocak, Aylin; Ozkan, Secil; Ozin, Yasemin; Bostanci, Birol; 31625359
    Background: Human papillomavirus (HPV), the causative agent of cervical cancer, is also suggested as a risk factor for gastric adenocarcinoma. Many infectious agents besides Helicobacter pylori have been associated with gastritis. The aim of this study was to investigate HPV DNA and genotyping HPV type 16 DNA in gastric adenocarcinoma and Helicobacter pylori gastritis cases. Methods: A hundred and six gastric adenocarcinoma and Helicobacter pylori gastritis samples and 26 controls were included. After deparaffinization by xylene, DNA extraction was performed by the phenol-chloroform-isoamyl alcohol method and 106 samples were studied with a G6PDH control kit (Eurogentec, Seraing, Belgium). Fifty-three adenocarcinoma and 43 Helicobacter pylori samples were thought to have enough tissue and were studied for HPV DNA. HPV types other than 16 and HPV type 16 DNA were detected by Real Time PCR using the L1 region. Amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV DNA and HPV 16 DNA specific probe in Light Cycler 2.0 (Roche Diagnostics, Germany) device. Results: Among gastric adenocarcinoma and Helicobacter pylori gastritis samples, 20/53 (38%) and 18/43 (41.8%) were HPV DNA positive, respectively. Five (19.2%) of 26 controls were HPV DNA positive. Conclusions: Our 38% positive result in the gastric carcinoma group is in concordance with previous reports. This is the first study revealing the HPV-H. pylori relationship in gastritis cases and we concluded that with regard to the nearly three-fold higher HPV DNA (41.8%) in gastritis cases compared to controls, Helicobacter pylori positive cases should also be evaluated in favor of HPV in the gastritis group.
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    Knowledge about Human Papilloma Virus (HPV) and HPV Vaccine at Reproductive Age in Primary Care
    (2015) Onder, Ozde; Dicle, Murat; Gulmez, Hakan; Aypak, Cenk; Akbiyik, Derya Iren; Gorpelioglu, Suleyman
    Purpose: The aim of this study was to identify the knowledge and awareness about human papilloma virus (HPV) and human papilloma virus (HPV) vaccine of women in reproductive age. Material and Methods: The study covered 294 women aged between 15 and 49. A questionnaire was prepared by the researchers based on the literature review. Results: The mean age of the participants was 30.5 +/- 8.9 years. Only 24.5% had heard about HPV infection and 28.2% had heard HPV vaccine. Of the participants, 188 (63.9%) got zero point from the knowledge questions. Conclusion: This study indicates that the women who apply primary care units have low knowledge levels; it is apperent that personal and social education is needed. Paying importance to patient education on HPV and cervical cancer in primary care health politics will increase knowledge and awareness for HPV infection and HPV vaccination.
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    HUMAN PAPILLOMA VIRUS (HPV) PREVALENCE AND GENOTYPE DISTRIBUTION
    (2017) Bolat, Filiz Aka; Colakoglu, Sule; Coban, Gonca; 0000-0002-3285-5519; AAI-9974-2021
    Assessment of Human papillomavirus (HPV) prevalence and genotype distribution is important for monitoring the impact of prophylactic HPV vaccination. This study aimed to demonstrate the HPV prevalence and type distribution in women from the Baskent University Adana Dr. Turgut Noyan Practice and Research Center. Cervical specimens from 268 women aged 22-68 years were collected between April 2014 and November 2015. Histopathological examinations were performed for 146 women. HPV DNA was amplified by PCR and HPV and genotyping was undertaken using the Roche (R) linear array detection kit. In total. 124 out of 268eligible samples (46.31%) tested positive for HPV. with the majority of these [84/124 (67.7%1] having high-risk (HR) HPV infection: 20.9% were positive for HPV16 (n=261, and 4% for HPV18 (n=5) HPV type-specific prevalence was 63.9% 53.39 and 80% among cervical intraepithelial neoplasias (CIN) Grades respectively. The coverage of other HR-HPV genotypes apart from 16. included HPV31. 45. 51, 53, and 56 in high-grade cervical lesions. In conclusion, HPV-16 was identified as the main HPV genotype associated with cervical disease in our hospital. The study reports the identification of high-and low-risk HPV genotypes as well as the prevalence of multiple HPV infections.