Scopus Açık Erişimli Yayınlar

Permanent URI for this collectionhttps://hdl.handle.net/11727/10760

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    The Phytochemical Profile and Biological Activity of Malva neglecta Wallr. in Surgically Induced Endometriosis Model in Rats
    (2022) Akkol, Esra Kupeli; Karpuz, Busra; Turkcanoglu, Gizem; Cosguncelebi, Fatma Gul; Tastan, Hakki; Aschner, Michael; Khatkar, Anurag; Sobarzo-Sanchez, Eduardo; 000887409800001
    Leaves and aerial parts of Malva neglecta Wallr. have been traditionally used in Anatolia for the treatment of pain, inflammation, hemorrhoids, renal stones, constipation, and infertility. This study investigated the effects of M. neglecta leaves in a rat endometriosis model. The dried plant material was extracted with n-hexane, ethyl acetate, and methanol, successively. Experimental endometriosis was surgically induced in six-week-old female, non-pregnant, Wistar albino rats by autotransplant of endometrial tissue to the abdominal wall. After twenty-eight days, rats were evaluated for a second laparotomy. Endometrial foci areas were assessed, and intraabdominal adhesions were scored. Rats were divided into five groups as control, n-hexane, ethyl acetate, methanol, and aqueous extracts, as well as reference. At the end of the treatment, all rats were sacrificed and endometriotic foci areas and intraabdominal adhesions were re-evaluated and compared with the previous findings. Moreover, peritoneal fluid was collected to detect tumor necrosis factor- alpha (TNF-alpha), vascular endothelial growth factor (VEGF), and interleukin-6 (IL-6) levels, and cDNA synthesis, and a quantitative real-time polymerase chain reaction (PCR) test was done. The phytochemical content of the most active extract was determined using High-Performance Liquid Chromatography (HPLC). Both endometrial volume and adhesion score decreased significantly in the group treated with methanol extract. In addition, significant decreases were observed in TNF-alpha, VEGF, and IL-6 levels in animals administered methanol extract. HPLC results showed that the activity caused by the methanol extract of M. neglecta was due to the polyphenols. Taken together, these novel findings indicate that M. neglecta may be a promising alternative for the treatment of endometriosis.
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    The Role of miRNA in Endometriosis
    (2021) Bilgili, Gamze; Verdi, Hasibe; Zeyneloglu, B. Hulusi; Tohma, Y. Aytac; Atac, F. Belgin
    Endometriosis is characterized by the presence of endometrial gland and stroma outside of the endometrial cavity. Not only the complex nature of disease limits the identification of the pathogenic mechanisms of endometriosis but the lack of simple diagnostic procedure is the major limitation. So identification of a simple clinical tool for the diagnosis of endometriosis has become a high priority research objective. MicroRNAs (miRNAs), a class of small noncoding RNA molecules, have been recognized as key post- transcriptional regulators those may associate with endometriosis. In the present paper, we aimed to review the studies which focused on the prognosis and diagnosis of the endometriosis and miRNAs. As studies on the role of miRNAs in the pathogenesis of endometriosis increase in the literature, new ideas can be put forward for early diagnosis, prevention and treatment of the endometriosis.