Scopus Açık Erişimli Yayınlar

Permanent URI for this collectionhttps://hdl.handle.net/11727/10760

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Author: search.filters.author.Bilgili, Gamzesearch.filters.applied.f.language: search.filters.language.eng

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    Is cervical swab an efficient method for developing a new noninvasive prenatal diagnostic test for numerical and structural chromosome anomalies?
    (2021) Yurtcu, Erkan; Karcaaltincaba, Deniz; Kazan, Hasan Huseyin; Ozdemir, Halis; Yirmibes Karaoguz, Meral; Calis, Pinar; Kayhan, Gulsum; Guntekin Ergun, Sezen; Percin, Ferda; Bayram, Merih; Ilhan, Mustafa Necmi; Bilgili, Gamze; Kaymak, Tugrul; Ergun, Mehmet Ali; Is cervical swab an efficient method for developing a new noninvasive prenatal diagnostic test for numerical and structural chromosome anomalies?; 0000-0003-4930-8164; 33315353
    Background/aim: Prenatal diagnosis is vital to obtain healthy generation for risky pregnancies. There have been several approaches, some of which are routinely applied in clinics to evaluate the possible prenatal deficiencies and/or diseases. In the present study, we aimed to isolate the fetal cells from endocervical samples and try to identify possible anomalies which were proved by Amniocentesis (AS) and chorionic villus sampling (CVS) methods. Materials and methods: Endoservical specimens were collected from 100 pregnant women. Cells were separated in parallel by fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS) using human leukocyte antigen (HLA) G233 and placental alkaline phosphatase (PLAP) antibodies. CMA (comprehensive meta-analysis) were carried out and male fetuses were confirmed with Sex determining region Y (SRY) amplification. Results: The percent of HLA G233 and placental and placental alkaline phosphatase (PLAP) positive cells were 4.55% and 84.59%, respectively. The percent of cells positive for both markers was 14.75%. CMA analyses were not informative. (SRY) was amplified in 67% of the samples. Conclusion: However, the success rate of the both cell sorting and scanning of DNA anomalies by aCGH and/or RT-PCR was limited, preventing the applicability of this proposal in the clinics. Still, the success of the proposed method depends on the development of the novel fetal cell-specific antibodies and the improvements in the sorting systems.
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    The Role of miRNA in Endometriosis
    (2021) Bilgili, Gamze; Verdi, Hasibe; Zeyneloglu, B. Hulusi; Tohma, Y. Aytac; Atac, F. Belgin
    Endometriosis is characterized by the presence of endometrial gland and stroma outside of the endometrial cavity. Not only the complex nature of disease limits the identification of the pathogenic mechanisms of endometriosis but the lack of simple diagnostic procedure is the major limitation. So identification of a simple clinical tool for the diagnosis of endometriosis has become a high priority research objective. MicroRNAs (miRNAs), a class of small noncoding RNA molecules, have been recognized as key post- transcriptional regulators those may associate with endometriosis. In the present paper, we aimed to review the studies which focused on the prognosis and diagnosis of the endometriosis and miRNAs. As studies on the role of miRNAs in the pathogenesis of endometriosis increase in the literature, new ideas can be put forward for early diagnosis, prevention and treatment of the endometriosis.