Kazan Meslek Yüksekokulu / Kazan Vocational School

Permanent URI for this collectionhttps://hdl.handle.net/11727/2077

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    Comparison Of Efficacy And Reliability Of Six Commercial Covid-19 Diagnostic PCR Kits
    (Başkent Üniversitesi Kazan Meslek Yüksek Okulu, 2024-03-26) Sharafi, Parisa; Gokdemir, F. Seyma; Akyol, Mesut; Ardicoglu-Akisin, Yasemin; Gocmen, J. Sedef; Taylan-Ozkan, Aysegul
    Objectives. All countries have been deeply affected by the coronavirus disease 2019 pandemic, both economically and in situations that strain health systems, such as workforce and workload. Therefore, various measures should be taken to control the disease and prevent its spread. Since the disease onset, real-time PCR tests have been used as the gold standard for disease diagnosis. Owing to the rapid progress of the pandemic and the spread of the disease, validation, consistency, and optimization tests of some commercial kits have been conducted directly in the field. Therefore, it is important to compare the results of these kits and improve the existing ones. Methods. We compared five kits (Bioexen, Polgen, Coronex, Diagen, and Anatolia) donated to the TOBB Economics and Technology University Hospital PCR laboratory with the KrosGen kit to detect severe acute respiratory syndrome coronavirus 2. A total of 244 samples were selected and analyzed using five different severe acute respiratory syndrome coronavirus 2 PCR detection kits. Results. Positive and negative results from the six kits were compared using the working protocols of the kits, primers, and cycle threshold (Ct) values. Five of the six kits have reliable compatibility for Ct<30 but decreases for Ct >= 30. Therefore, it is important to evaluate the performance of these kits for reduced viral loads. Conclusions. Using a suitable kit with high compatibility for Ct >= 30 is important for detecting patients with a low viral load and helping prevent disease spread.
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    Evaluating The Multifaceted Impact Of Cephalaria Extract On Dough Quality: Antioxidant, Antimicrobial, And Cytotoxic Properties
    (INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2024-12) Bagdat, Elif Seyma; Bozkurt, Fatih; Ilikkan, Ozge Kahraman; Sagdic, Osman; Tornuk, Fatih
    Cephalaria syriaca, known as cephalaria, is an annual, pilose plant with pink-purple flowers growing wild in wheat fields. Viscoelastic properties of gluten-free doughs can be improved by adding cephalaria. However, this addition results in some undesirable properties in bread such as bitter taste and dark internal colour. This study aimed to reduce these effects by obtaining cephalaria obtained from distilled water and ethanol extracts and to increase the potential use of extracts in bread production. Different proportions of water and ethanol extracts obtained from oil-free cephalaria were added to bread by creating a model with Mixture Design, and the optimum concentration was determined by farinograph trials. The antioxidative, antimicrobial, and cytotoxic effects of extract and the effect of cephalaria on yeast activity were examined. Cephalaria extracts had no measurable antimicrobial effect and no antifungal effect on Rhizopus stolonifer, while an antifungal effect was found against A. niger and P. expansum. The IC50 value was found to be 4.15 mg mL(-1 )for ethanol extract while the water extract had no effect on cells. The addition of extract had no negative effect on the number of yeasts in sourdough fermentation. The study has improved the usage of cephalaria by using water and ethanol extracts in dough.
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    A Novel Synbiotic Edible Film Based On Aquafaba, Psyllium Husk Powder, Peg 400, And Lactiplantibacillus Plantarum 299v And Applicability On Kashar Cheese
    (JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2024-12) Ilikkan, Ozge Kahraman; Bagdat, Elif Seyma; Yilmaz, Ilkay; Beyter, Nurten; Yuzay, Isinay Ebru
    In this study, it was aimed to compare a novel synbiotic edible film based on aquafaba, psyllium husk powder, and PEG 400 with or without Lactiplantibacillus plantarum 299v. Therefore, primarily, optimization of the edible film was carried out by Box-Behnken design in response surface methodology (rsm). The characterization of edible films with or without probiotic bacteria was performed by FTIR, viscosity, pH, total solid content, water and oxygen solubility, film thickness, color L*, a*, b*, transparency, and film tensile strength. It was determined that there was no statistically significant difference in viscosity, pH, lightness L*, water vapor, and oxygen permeability values with the addition of probiotic lactic acid bacteria to the optimized coating film samples. In addition, it was observed that the probiotic bacteria count did not decrease below 108 cfu/g for 60 days of storage. The resulting edible film formulation with (PL) and without (PS) probiotics was used to coat Kashar cheese, while control cheeses (C) were not coated. As a result, it was observed that the microbiological quality of the coated cheeses was as good as control or better than control especially at the end of storage in terms of mold/ yeast and coliforms. Sensory evolution revealed coating films with or without probiotics did not change the texture, flavor, odor, or appearance of cheeses. Physicochemical properties such as pH, % titratable acidity, % dry matter, % fat, and % salt were detected.