Başkent Üniversitesi Makaleler

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Subject: search.filters.subject.Bone marrow transplant

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    The IFN-γ Allele Is Correlated to Moderate-to-Severe Acute Graft-Versus-Host Disease After Allogeneic Stem Cell Transplant
    (Başkent Üniversitesi, 2010-06) Yaghobi, Ramin; Ebadi, Padideh; Karimi, Mohammad Hossein; Daneshmandi, Saeed; Pourfathollah, Ali Akbar; Geramizadeh, Bita; Ramzi, Mani
    Objectives: Analysis of nonhistocompatibility leucocyte antigen functional genomics in stem cell transplant can lead to prediction of clinical outcomes in histocompatibility leucocyte antigen-matched sibling-transplant recipients. Some of the cytokine gene polymorphisms might be associated with severe, acute graft-versus-host disease after allogeneic stem cell transplant. We evaluated gene polymorphisms of IL-6 G-174C, TGF-ß T+869C, IL-4 C-590T, and IFN-γ T+874A cytokines in bone marrow transplant patients. Materials and Methods: The Amplification refractory mutation system-polymerase chain reaction ARMS-PCR method was used to characterize IL-6 G-174C, TGF-ß T+869C, and IFN-γ T+874A polymorphisms, and PCR-RFLP, using AvaII restriction enzyme, was done for IL-4 C-590T characterization in 35 bone marrow transplant patients. Acute graft-versus-host disease episodes were diagnosed according to EMBT criteria. Results: Analysis showed that IFN-γ +874T allele (P = .027, OR=0.198, 95% CI=0.049-0.801) was correlated to moderate-to-severe graft-versus-host disease. TGF-ß T+869C, IFN-γ T+874A, IL-6 G-174C and IL-4 C-590T frequencies were not significantly different in the 2 graft-versus-host disease severity groups (P > .05). Conclusion: According to the results, we concluded that the IFN-γ T+874A gene polymorphism has a predictive value for severity of graft-versus-host disease after bone marrow transplant. High producer genotypes of IFN-γ are genetic risk factors for development of graft-versus-host disease.
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    Quantification of Human Cytomegalovirus DNA by a New Capture Hybrid Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay in Plasma and Peripheral Blood Mononuclear Cells of Bone Marrow Transplant Recipients
    (Başkent Üniversitesi, 2008-12) Ziyaeyan, Mazyar; Kadivar, Mohammad; Pourabbas, Bahman; Mahboudi, Fereidoun; Ramzi, Mani; Alborzi, Abdolvahab; Sabahi, Farzaneh
    Objectives: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients. Patients and Methods: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant, was evaluated in the study. A newly designed biotinylated probe-mediated quantitative competitive PCR-ELISA test was used to determine cyto­megalovirus load in specimens of peripheral blood mononuclear cells and plasma. Results: All 26 patients were cytomegalovirus seropositive before transplant. Capture hybrid PCR-ELISA of peripheral blood mononuclear cells detected cytomegalovirus DNA in 287 of 313 specimens (91.7%) even in cases with no active cytomegalovirus infection. in plasma, cyto­megalovirus DNA was detected in 114 of 313 specimens (36.4%). Increasing titers of cyto­megalovirus DNA were detected in 14 of 26 patients (53.8%). Conclusions: The quantitative capture hybrid PCR-ELISA was able to diagnose and monitor cytomegalovirus infection in patients who received bone marrow transplants. Detection of cyto­megalovirus DNA in plasma was more predictive of the onset of cytomegalovirus-related clinical symptoms, compared to detection in peripheral blood mononuclear cells.