Browsing by Author "Yilmaz, Soner"

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    Prevalence and Quantity of Parvovirus B19 DNA Among Blood Donors from a Regional Blood Center in Turkey
    (2020) Guclu, Aylin Uskudar; Yilmaz, Soner; Baysallar, Mehmet; Avci, Ismail Yasar; 0000-0002-1872-028X; 32439492; AAU-6196-2020
    Objective: Parvovirus B19 causes a range of diseases and morbidity in humans and is transmissible by transfusion of blood, blood components and plasma derivatives. The objective of the study was to investigate the prevalence and quantity of B19 DNA among blood donors. Method: Totally 1053 samples were collected from March to July 2016 at a blood bank for detection of Parvovirus B19 DNA and serological status of blood donors. Testing of the presence of viral DNA was performed by a quantitative real-time PCR with a 101 copies/ml detection limit. All DNA positive and randomly selected 267 samples were tested for the presence of anti-B19 IgM and IgG by ELISA. Results: Age distribution of donors was between 18-64; mean age was 27 and median was 23. Among the 1053 samples, 5 (0.47%) had PB19 DNA. All PB19 DNA positive donations had both B19 IgM and IgG antibodies. The DNA level for positive donations were between 0.9 x 102 to 3.1 x 104 copies/ml. IgG and IgM were present in 59.9% (160/267) and 0,74% (2/267) respectively among the healthy donors without PB19 DNA. Conclusion: Detected DNA concentration was less than 105 copies/ml. The presence of IgM in low level PB19 DNA positive donors may indicate that there might be a risk in transmission of PB19 to particularly immunosuppressed recipients. The clinical follow-up of blood donation with low level of PB19DNA should be considered to answer the questions about blood safety.
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    Prevalence of CMV DNA in Blood Donors in a Regional Blood Center in Turkey
    (2022) Uskudar Guclu, Aylin; Yilmaz, Soner; Baysallar, Mehmet; Ayci, Ismail Yasar; 0000-0002-1872-028X; AAU-6196-2020
    Cytomegalovirus (CMV) infections with a high seroprevalence rate in adults are often asymptomatic or mild. The current prevalence of CMV DNA in Turkey is not known. This study aimed to find out the prevalence and quantity of CMV DNA in blood donors by PCR. Totally 1003 samples were collected between March and June 2016 at a regional blood bank for detection of CMV DNA in healthy blood donors by quantitative real-time PCR. CMV-DNA positive samples were tested for the presence of anti CMV IgM and IgG by ELISA. Among 1003 donors, 973 (97.01%) were male and 30 (2.99%) were female. Age distribution of donors was between 18-64; mean age was 27. Most of the donors were between 18-30 years old (75.47%) and male (97.01%). One donor was found as CMV DNA positive (0.099%). The DNA quantity for positive donation was 1,75x10(2) IU/ml. CMV DNA positive sample was positive for IgG, whereas negative for IgM. Although the seroprevalence of CMV is high in our country the CMV DNA prevalence was found very low (0.099%). Testing of CMV DNA in blood donation particularly for some specific patient groups and the clinical follow-up of blood donation with low level of CMV DNA should be considered.