Browsing by Author "Yildirim, Derya"

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Imaging Characteristics of Stafne Bone Cavity: Pictorial Essay
(2017) Aydin, Ulkem; Yildirim, Derya; Gormez, Ozlem; Bozdemir, Esin; Yilmaz, Omer; Erik, Ayse Aydogmus; Orhan, Mukkadder
Background: Stafne bone cavities (SBCs) are typically seen on panoramic radiographs as unilocular, rounded or ovoid shaped, welldefined corticated radiolucencies that are located between the mandibular first molar and the angle of the mandible below the inferior alveolar canal, but they may rarely have different radiographic appearances and locations. Objectives: The purpose of this study was to evaluate the imaging features of SBC presenting various typical and atypical features and to show the contribution of different imaging techniques to diagnosis. Patients and Methods: Seventeen patients who had a panoramic radiograph that revealed an image compatible with SBC were investigated in this study. In addition to panoramic radiography, lateral oblique mandible projection for three patients, cone beam computed tomography (CBCT) for nine patients, magnetic resonance imaging (MRI) for two patients were performed to determine the exact location of the cavity and to confirm the diagnosis. Results: Seventeen patients were diagnosed with SBC. Two patients had bilobed SBC, one patient had a SBC on the buccal surface of the posterior mandible, one patient had a SBC located in the ramus mandible, one patient had a SBC located in the canine-premolar region namely anterior lingual variant as rare conditions. Conclusion: Imaging techniques such as CBCT and MRI have provided detailed information about definitive diagnosis of SBC in addition to panoramic radiographs. These techniques show the size, location and content of the SBC. If the SBC is atypical, complementary imaging techniques gain more importance.
Role of autophagy and evaluation the effects of microRNAs 214, 132, 34c and prorenin receptor in a rat model of focal segmental glomerulosclerosis
(2021) Yildirim, Derya; Bender, Onur; Karagoz, Zehra Firat; Helvacioglu, Fatma; Bilgic, Mukadder Ayse; Akcay, Ali; Ruzgaresen, Nuket Bavbek; 0000-0002-6026-0045; 34087284
Aims: Focal segmental glomerulosclerosis (FSGS) is the common cause of chronic renal disease worldwide. Although there are many etiologic factors which have common theme of podocyte injury conclusive etiology is not clearly understood. In this study, we aimed to explore the role of autophagy in the pathogenesis of podocyte injury, which is the key point in disease progression, and the roles of intrarenal microRNAs and the prorenin receptor (PRR) in the 5/6 nephrectomy and adriamycin nephropathy models of FSGS. Main methods: For experimental FSGS model, 5/6 nephrectomy and adriamycin nephropathy models were created and characterized in adult Sprague Dawley rats. Microarray analysis was performed on FSGS and control groups that was confirmed by q-RT-PCR. Beclin1, LC3B, PRR, ATG7 and ATG5 expression were evaluated by western blotting and immunohistochemistry. Also, Beclin1 and PRR expression were measured by ELISA. Glomerular podocyte isolation was performed and autophagic activity was evaluated in podocytes before and after transfection with miRNA mimic and antagonists. Key findings: Glomerular expression of Beclin1, LC3B, PRR, ATG7 and ATG5 were significantly lower in the 5/6 nephrectomy than adriamycin nephropathy group and in both groups lower when compared to control groups. Western blot results were consistent with immunohistochemical data. Electron microscopy revealed signs of impaired autophagy in FSGS. Autophagic activity decreased significantly after miR-214, miR-132 and miR-34c mimics and increased after transfection with antagonists. Significance: These results showed that the role of autophagic activity and decreased expression of PRR in FSGS pathogenesis and miR-34c, miR-132 and miR-214 could be a potential treatment strategy by regulating autophagy.