Browsing by Author "Yigit, Ayse Arzu"

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Assessment of tebuconazole exposure on bovine testicular cells and epididymal spermatozoa
(2021) Kabakci, Ruhi; Kaya, Abdulkadir; Yigit, Ayse Arzu; Varisli, Omer; 0000-0001-5837-6877; 34214047; C-2636-2017
This study is the first to investigate the effects of tebuconazole (TEB) on the physiological functions of bovine testicular cells and epididymal spermatozoa. Motility and plasma membrane integrity of spermatozoa exposed to TEB (0.001-100 mM) were evaluated at different incubation times (0-6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from the parenchyma of bovine testes, were seeded at 1.0 x 10(4) and 1.5 x 10(6) cells/well in 96- and 12-well culture plates and incubated for 48 h in culture media containing TEB (0.001- 100 mM) to evaluate cytotoxicity and hormone release, respectively. TEB did not affect the motility and plasma membrane integrity. However, significant spermiotoxicity occurred at higher TEB (1-100 mM) concentrations (P < 0.05) compared to control and lower doses. Although no dose caused cytotoxicity in testicular cells (P > 0.05), 1 and 100 mM TEB caused a significant increase in testosterone secretion (P < 0.05). As a result, high doses of TEB (1-100 mM) had slightly suppressive effects on spermatozoa; however, these doses had stimulatory effects on testosterone secretion by testicular cells. It appears that the disruption of hormonal homeostasis of testicular cells after TEB exposure may result in metabolic and especially reproductive adverse effects in bulls.
The Effect of Ginkgo Biloba on Anxiety and Oxidative Stress Parameters in Healthy Young Rats
(2023) Arnous, Elif Azra; Yigit, Ayse Arzu
Effects Of Incubator Oxygen And Carbon Dioxide Concentrations On Hatchability Of Fertile Eggs, Some Blood Parameters, And Histopathological Changes Of Broilers With Different Parental Stock Ages In High Altitude
(2022) Okur, Nezih; Eratalar, Sabri Arda; Yigit, Ayse Arzu; Kutlu, Tuncer; Kabakci, Ruhi; Ozsoy, Sule Yurdagul; https://orcid.org/0000-0001-5837-6877; 34936960; C-2636-2017
The effects of incubator carbon dioxide (CO2) and oxygen (O-2) concentrations with parental stock age (PSA) on embryonic deaths (ED), hatchability of fertile eggs (HFE), some blood parameters, and the tissue development of broilers were investigated. Four consecutive repetitions following the similar materials and methods were carried. From 3 different aged ROSS 308 broiler parental flocks 7,680 hatching eggs were obtained and classified as young (Y; 29 wk), middle (M; 37 wk) and old (O; 55 wk) as regards PSA, and randomly distributed. Four different incubator ventilation programs (IVP) as control (C; 0.67% CO2 and 20.33% O-2), high CO2 (HC; 1.57% CO2 and 20.26% O-2), high O-2 (HO; 0.50% CO2 and 21.16% O-2), and high CO2 + O-2 (HCO; 1.17% CO2 21.03% O-2) were applied with oxygen concentrator, and ED and HFE were investigated. Lung and heart tissues, hemoglobin value, packed cell volume, and red blood cell count, triiodothyronine, thyroxine, adrenocorticotropic hormone (ACTH) values of the chicks were analyzed. It was found that IVP affected ED and HFE. Higher rate of early ED (EED) was obtained from the HC than HCO, and higher middle+late stage +pipped but unhatched ED (MLPED) with a lower rate of HFE was observed in the C group than HO and HCO (P < 0.05). Association was found between PSA and IVP (P < 0.05), being more evident in EED for young PSA, in MLPED with HFE for Y and O PSA. From hematological values, no statistical difference in RBC, PCV, and Hb values were found among the treatment groups, ACTH concentration known as a response to stress was found to be higher than C in all groups, triiodothyronine concentration was higher in the HO group than C. In the histopathological examination, used IVPs were found to have negative effects on the lung and heart such as vacuolization, hemorrhage in all PSA groups except for C. Conclusively, PSA and IVP affected some hatching, blood and tissue development parameters of the broiler chicks.
The Effects of Orlistat on Spatial Memory, Recognition Memory and Hippocampal Tissue in Experimentally Induced Obesity in Rats
(2023) Yigit, Ayse Arzu; Kilinc, Sevtap; Olcuoglu, Rukiye; Arnous, Elif Azra; 0000-0002-0429-008X; 0000-0002-4162-1554; JZJ-5475-2024; ISP-2968-2023
Evaluation of Electrodermal Activity and Anxiety Behaviors in Diabetic Rats Given Vildagliptin and Metformin
(2022) Shawesh, Muftah; Alshareef, Mohammed; Boyuk, Gulbahar; Yigit, Ayse Arzu; Dolu, Nazan
The Impact of Orlistat on Nitrosative Stress and Paraoxonase-1 Enzyme Activity in Serum, Heart, Kidney, and Testicular Tissues of Rats with Experimentally Induced Obesity
(2023) Kilinc, Sevtap; Olcuoglu, Rukiye; Yigit, Ayse Arzu; 0000-0002-0429-008X; 0000-0002-4162-1554; JZJ-5475-2024; ISP-2968-2023
Investigation of cochlear microphonics recorded with different stimulus types
(2022) Guneser, Ozgecan; Yigit, Ayse Arzu; Alniacik, Asuman; Yanarates, Kursat; Cakmak, Eda; 0000-0001-5837-6877; 36326414; C-2636-2017
Background/aim: Electrocochleography (ECochG), one of the first defined tests under auditory evoked potentials, is a total electrical response of inner and outer hair cells inside the cochlea and auditory nerve record technique to the presence of an acoustic stimulus. These records can be used in Meniere disease and auditory neuropathy spectrum disorder diagnosis, intraoperative monitoring. In addition, the presence of cochlear microphonics plays a crucial role in auditory neuropathy spectrum disorder diagnosis. In our study, healthy individuals were tested with extratimpanic electrocochleography record method via Click and LS CE-Chirp stimulus, and the results were compared to the age, sex, and noise sensitivity categories. Materials and methods: This study had executed at Baskent University, Faculty of Health Sciences Audiology laboratory. The study group consisted of 42 volunteers between 18 and 40 years old. To understand the suitability of volunteers, pure tone audiometry, tympanometry, and transient otoacoustic emission tests were performed. Individuals with no hearing loss were tested with 100 dBnHL intensity level via click and LS CE-Chirp stimulus. The obtained values were statistically evaluated in the SPSS 23.0 program in accordance with the data distribution. An independent sample t-test was used for data showing normal distribution, and Mann-Whitney U test was used for data not showing normal distribution. The level (p < 0.05) was considered statistically significant for all analyses performed. Results: Cochlear microphonic amplitudes recorded with click and LS CE-Chirp stimuli were higher in males than in females (p = 0.051 and p = 0.001, respectively). When the age groups were evaluated, no difference was observed in the CM amplitudes obtained with both click and LS CE-Chirp stimuli. There was no correlation between age and CM amplitudes. Additionally, it was determined that the CM amplitudes recorded with the click stimulus in individuals with noise sensitivity were higher than those without noise sensitivity (p = 0.051). Conclusion: It is thought that the ECochG amplitudes of different gender, different age, and different noise sensitivity, which are the results of our study, can be used in the diagnosis of diseases such as auditory neuropathy spectrum disorder.
Investigation of the Achievability of Stem Cells Derived from Luteal Cells in Rats
(2018) Yigit, Ayse Arzu; Orscelik, Emine Umran; Boyuk, Gulbahar; Tiryaki, Meral; Pinarli, Ferda Alparslan; 0000-0002-3453-2967; AAC-4677-2021
Investigation of the Effects of Metribuzine on Cow Granulosa Cell Steroidogenesis
(2022) Yigit, Ayse Arzu; Kahveci, Metehan Eser; Kabakci, Ruhi
A Preliminary Study on the Effect of Age and Gender to the Amplitudes of Cochlear Microphonics Recorded by Click Stimulus in Individuals with Normal Hearing
(2022) Guneser, Ozgecan; Alniacik, Asuman; Yigit, Ayse Arzu; Koycu, Alper; Cakmak, Eda; Yanarates, Kursat; https://orcid.org/0000-0003-1290-3509; AAF-3650-2021
The Effects Of Orlistat On Oxidative Stress, Recognition Memory, Spatial Memory And Hippocampal Tissue In Experimentally Induced Obesity In Rats
(Başkent Üniversitesi Sağlık Bilimleri Fakültesi, 2024-03-22) Yigit, Ayse Arzu; Kilinc, Sevtap; Olcuoglu, Rukiye; Arnous, Elif Azra
This study investigates the impact of orlistat on oxidative stress, spatial memory, recognition memory, and hippocampal tissue in obese rats. The study groups were divided into control, high fat diet-induced obese (HFDIO), HFDIO+orlistat (HFDIO+ORL) groups, each consisting of 8 animals. While control fed with standart diet, HFDIO and HFDIO+ORL fed with high-fat diets for 8 weeks to induce obesity. Then, ORL treated 10 mg/kg for 7 weeks, while control and HFDIO get water. At 16th week, novel object recognition (NOR) and Morris water maze (MWM) tests were performed. TNF-alpha, IL-1beta levels in hippocampal tissue, and total/native thiol/ disulphide levels in serum were measured. TNF-alpha level of HFDIO was higher than control, while lower in HFDIO+ORL compared to HFDIO as like IL-1beta level. On the contrary, serum total thiol level was lower in HFDIO than control and higher in HFDIO+ORL compared to the HFDIO, while disulphide level was opposite of the total thiol levels. While recognition index was higher in HFDIO+ORL, in MWM, latency of finding platform in HFDIO was higher than control and latency of HFDIO+ORL was very similar to control in 2-4 days. The HFDIO group demonstrated decrease in time spent in platform zone compared to control, whereas time spent of the HFDIO+ORL was higher than HFDIO. Our study demonstrates that orlistat administration exerts beneficial effects on oxidative stress, spatial memory, recognition memory, and hippocampal tissue in obese rats. It shows that orlistat may have potential therapeutic implications for obesity-related cognitive impairments and hippocampal dysfunction.