Browsing by Author "Yalcin, Yaprak"

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    Beta-Cell Golgi Stress Response to Lipotoxicity and Glucolipotoxicity: A Preliminary Study of a Potential Mechanism of Beta-Cell Failure in Posttransplant Diabetes and Intraportal Islet Transplant
    (2022) Tutuncu, Neslihan Bascil; Verdi, Hasibe; Yalcin, Yaprak; Cebi, Pinar Baysan; Kinik, Sibel; Tutuncu, Tanju; Atac, Fatma Belgin; 0000-0002-1816-3903; 0000-0002-9337-9106; 0000-0002-9141-9987; 35791832; ABG-5027-2020; ABB-4078-2020
    Objectives: Lipotoxicity and glucolipotoxicity are among the most important triggers of beta-cell failure in patients with type 2 and posttransplant diabetes. Because the Golgi apparatus is a vital organelle in secretory cells like beta cells, its behavior under stress conditions determines the cell's functional capacity.Materials and Methods: To mimic lipotoxicity and glucolipotoxicity as metabolic stresses for beta-cell failure, rat insulinoma INS-1E cells were treated with palmitic acid, glucose, or both. Cells were cultured in the presence of 5.0, 16.7, or 33 mM glucose with or without 0.5 mM palmitic acid for 8, 16, 24, and 48 hours. Incubation in the presence of any of the 3 concentrations of glucose with 0.5 mM palmitic acid provided glucolipotoxicity. In addition to the endop-lasmic reticulum stress marker (Hspa5), we evaluated changes in Golgi function under experimental metabolic stresses. In doing this, we measured expression levels of the genes coding Golgi structural proteins (Acbd3, Golga2, and Arf1), Golgi glycosylation enzymes sialyltransferaz10 and sialyltransferase 1 (St3gal1), and Golgi stress mediators (Creb3 and Arf4).Results: Golgi responded to lipotoxicity and glucolipotoxicity by increasing the expression of St3gal1 (P = .05 in both conditions) and Creb3 (P = .022 and P = .01, respectively). The Arf4 gene transcript also increased in glucolipotoxic media (P = .03). Glucotoxicity alone did not induce a change in the transcript levels of Creb3 and Arf4. Lipotoxicity and glucolipotoxicity induced Creb3 and Arf4 expression, which are important Golgi stress response mediators leading to apoptosis.Conclusions: This preliminary study showed that the Golgi stress response is important in lipotoxic and glucolipotoxic conditions in terms of beta-cell failure. Solving the mystery of intracellular molecular mechanisms leading to beta-cell dysfunction is crucial to understanding the pathophysiology of posttrans-plant diabetes and most probably the failure of intraportal islet transplants in the long term.
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    Effect of Maternal and Neonatal Interleukin-6-174 G/C Polymorphism on Preterm Birth and Neonatal Morbidity
    (2018) Karakas, N. Mutlu; Ecevit, Ayse N.; Yalcin, Yaprak; Ozdemir, Beril; Verdi, Hasibe; Tekindal, M. Agah; Ozbek, Namik Y.; Tarcan, Aylin; Atac, Fatma B.; Haberal, Ali; 0000-0002-9337-9106; 0000-0003-4286-7086; 0000-0001-6857-0681; 0000-0002-4060-7048; 0000-0002-2232-8117; 0000-0003-0591-009X; 0000-0002-1486-7209; 0000-0001-6868-2165; 28279124; ABB-4078-2020; AAX-3831-2020; HPC-6496-2023; U-9270-2018; AAJ-4616-2021; ABG-9940-2020; AAI-9331-2021; ABG-9966-2020
    Objective: The aim of this study was to analyze maternal and neonatal interleukin 6 (IL-6) (-174 G/C) polymorphism and to determine effect on preterm birth and neonatal morbidity. Study Design: One hundred and sixty-four mothers (100 term births, 64 preterm births) and 183 newborn infants who were 100 healthy term and 83 preterm babies followed in newborn intensive care units were evaluated. PCR-RFLP was performed for IL-6 (-174 G/C) genotyping. Results: The rate of GG genotype in mothers of term and preterm infants were 54% (n = 54/100), 75% (n = 48/64), respectively (p>.05) and the rate of GC+CC genotype was 46% (n = 46/100) and 25% (n = 16/64) in mothers giving term and preterm birth (PTB), respectively (p<.05). Additionally, the rate of GG genotype was 65% (n = 65/100) and 81.9% (n = 68/83) in term infants and preterm infants, respectively. GC+CC genotype was 35% (n = 35/100) in term infants and 18.1% (n = 15/83) in preterm infants (p<.05). The effect of IL-6 (-174) GC+CC genotype on PTB was statistically significant. Conclusion: The IL-6 174 G/C gene polymorphism was significantly different between mothers who were giving to term and preterm birth. The presence of polymorphism is protective against preterm birth and was not associated with neonatal outcome.
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    Effects of Natural Immune System on Acute Rejection of Living Donor Renal Transplantation
    (2016) Kirnap, Mahir; Yalcin, Yaprak; Ozcelik, Umit; Ekici, Yahya; Fatac, Belgin; Moray, Gokhan; Haberal, Mehmet; https://orcid.org/0000-0002-9337-9106; https://orcid.org/0000-0003-1073-2494; https://orcid.org/0000-0003-2498-7287; https://orcid.org/0000-0002-3462-7632; AAH-9198-2019; ABB-4078-2020; AAG-8651-2021; AAE-1041-2021; AAJ-8097-2021