Browsing by Author "Wang, Guodong"

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Comparison of 2 Heterotopic Heart Transplant Techniques in Rats: Cervical and Abdominal Heart
(Başkent Üniversitesi, 2011-04) Ma, Yi; Wang, Guodong
Objectives: Heterotopic heart transplant in rats has been accepted as the most-commonly used animal model to investigate the mechanisms of transplant immunology. Many ingenious approaches to this model have been reported. We sought to improve this model and compare survival rates and histologic features of acute rejection in cervical and abdominal heart transplants. Materials and Methods: Rats were divided into cervical and abdominal groups. Microsurgical techniques were introduced for vascular anastomoses. In the abdominal heart transplant group, the donor’s thoracic aorta was anastomosed end-to-side to the recipient’s infrarenal abdominal aorta, and the donor’s pulmonary artery was anastomosed to the recipient’s inferior vena cava. In the cervical heart transplant group, the donor’s thoracic aorta was anastomosed to the recipient’s common carotid artery, and the donor’s pulmonary artery was anastomosed to the recipient’s external jugular vein. Survival time of the 2 models was followed and pathology was examined. Histologic features of allogeneic rejection also were compared in the cervical and abdominal heart transplant groups. Results: The mean time to recover the donor’s hearts was 7.4 ± 2.2 minutes in the cervical group and 7.2 ± 1.8 minutes in the abdominal group. In the cervical and abdominal heart transplant models, the mean recipient’s operative time was 23.2 ± 2.6 minutes and 21.6 ± 2.8 minutes. Graft survival was 98% and 100% in the cervical and abdominal heart transplant groups. There was no significant difference in graft survival between the 2 methods. Heart allografts rejected at 5.7 and 6.2 days in the cervical and abdominal transplant groups. There was no difference in the histologic features of acute allogenic rejection in cervical and abdominal heart transplant. Conclusions: Both cervical and abdominal heart transplants can achieve a high rate of success. The histologic features of acute allogeneic rejection in the models are comparable.
Hepatic Artery Thrombosis After Orthotopic Liver Transplant: A Review of the Same Institute 5 Years Later
(Başkent Üniversitesi, 2011-06) Wu, Linwei; Hu, Anbin; Wang, Guodong; Ma, Yi; Zhu, Xiaofeng; Wang, Dongping; Ju, Weiqiang; He, Xiaoshun; Tai, Qiang; Guo, Zhiyong; Zhang, Jianwei
Objectives: Summarize the experience of managing patients with hepatic artery thrombosis after orthotopic liver transplant in a single center. Materials and Methods: A total of 726 adult patients who received a liver transplant at the Department of Organ Transplantation, the First Affiliated Hospital of Sun Yat-Sen University, between January 2004 and December 2009, were selected. Fourteen patients had hepatic artery thrombosis after the operation, and the clinical data of these patients were analyzed retrospectively. Results: The incidence rate of hepatic artery thrombosis was 1.9% (14/726), and the mean time of onset was 10 days (range, 1 - 41 d) after surgery. Six patients had acute deterioration of liver function, 4 had bile leakage, 1 had hepatic abscess, and 3 had no symptoms. Three patients received urgent rearterialization, 2 received intra-arterial thrombolysis, 3 received combined urgent rearterialization and intra-arterial thrombolysis, and 6 patients received a retransplant. The mortality rate associated with hepatic artery thrombosis was 42.9% (6/14); 2 from biliary necrosis and secondary hepatic failure after urgent rearterialization; 1 from recurrent hepatic artery thrombosis and multiple organ failure after intra-arterial thrombolysis; 1 from renal failure and severe infection after combined urgent rearterialization and intra-arterial thrombolysis, and 2 from severe infection after retransplant. The other patients recovered and were followed for 18 to 66 months. Their liver grafts all functioned well with a patent artery. Two died from tumor recurrence at 18 and 29 months after transplant. Conclusions: Hepatic artery thrombosis is a severe complication after liver transplant, which leads to graft loss and recipient death. Rearterialization as early as possible before irreversible biliary and liver parenchyma damage can avoid retransplant.