Browsing by Author "Uckan, Duygu"

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    Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation
    (2016) Mendi, Aysegul; Kose, Sevil; Uckan, Duygu; Akca, Gulcin; Yilmaz, Dervis; Aral, Levent; Gultekin, Sibel Elif; Eroglu, Tamer; Kilic, Emine; Uckan, Sina; 27008259
    An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01). Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.
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    Thrombotic Microangiopathy in Allogeneic Stem Cell Transplantation in Childhood
    (Başkent Üniversitesi, 2010-09) Erbey, Fatih; Tanyeli, Atila; Uckan, Duygu; Cetin, Mualla; Yilmaz, Sema; Kuskonmaz, Baris; Bayram, Ibrahim
    Objectives: We define the incidence, risk factors, and mortality rates for the occurrence of thrombotic microangiopathy in 50 children who underwent transplants between January 2006 and June 2008 at 2 Turkish pediatric centers. Materials and Methods: The diagnosis of thrombotic microangiopathy was done according to the reports of International Working Group in 2007. Results: Fifty patients (27 male and 23 female; age range, 3 months to 18 years) were included. Patients with malignant and nonmalignant diseases were 13 (26%) and 37 (74%). Myeloablative and nonmyeloablative conditioning regimens were used in 29 (58%) and 21 patients (42%). Bone morrow was used as the source of stem cells in 32 patients (62%) and peripheral blood was used in 18 patients (36%). Thrombotic microangiopathy was seen in 3 of 50 cases (6%). Thrombotic microangiopathy developed in 3 of 18 patients in whom peripheral blood was used as the source of stem cells while none of 32 patients who had bone marrow as the source developed thrombotic microangiopathy (P < .05). Conclusions: Using peripheral blood as a source of stem cells is a risk factor for development of thrombotic microangiopathy.