Browsing by Author "Toprak, Ugur"

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    Effects of Zinc Oxide Nanoparticles on Neural Tube Development in Early Chicken Embryos
    (2023) Ayran Fidan, Pinar; Efe, Ekin; Toprak, Ugur; Aynur, Julide; Hamsici, Yagmur; Coban, Beyza Nur; Canta, Sinem; Ozer, Reyyan Sena; 0000-0002-2949-9189; 0000-0003-2855-6695; 37309631; AAL-3180-2021
    AIM: To investigate the effect of zinc oxide nanoparticles (ZnO-NPs) on neural tube development in early chicken embryos.MATERIAL and METHODS: Fifty pathogen-free fertilized eggs were initially incubated for thirty hours. The eggs were divided into 5 groups. In the control group (C) the egg's apex was opened and closed without any administration. In the distilled water group (DW), 10 microliters of distilled water were injected into the sub-blastodermic area. ZnO-NP suspensions were prepared in distilled water and injected sub-blastodermically into the low, medium and high dose ZnO-NP groups (10 mg/kg, 30 mg/kg, and 50 mg/kg, respectively). Incubation was completed in 72 hours, and embryological and neural tube development was evaluated histologically with a light microscope.RESULTS: Embryos in all groups were evaluated according to the Hamburger-Hamilton (HH) staging. It was observed that the staging progressed by the developmental process between 68-72 hours, which is equivalent to the 19-20th stage of HH. Differentiated otic vesicle, optic cup, lens vesicle, pharynx, and Rathke's pouch were all observed in embryo sections. Both forebrain and hindbrain vesicles were easily distinguished in the sections by cranial flexion. Neural tube closure defect was not detected in any of the groups.CONCLUSION: In our observations, ZnO-NPs did not affect neural tube development at the applied dose ranges. We believe that additional studies with higher doses using a higher number of subjects will help clarify the conflicting data in the literature.
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    Relationship Between Asthma and IL-17 Gene Polymorphism in A Turkish Population
    (2023) Yuce, Gulbahar Darilmaz; Erdogan, Tuba; Bozkurt, Bulent; Toprak, Ugur; Ceylan, Gulay Gulec; https://orcid.org/0000-0002-3143-2442; https://orcid.org/0000-0002-2949-9189; 35325374; JBS-4193-2023; ABH-5354-2020; AAL-3180-2021
    Background Asthma is a prevalent chronic obstructive disease of the airways. Aims The aim of our study was to investigate the relationship between asthma and IL-17F gene 74488 T > C, IL-17A gene -197G > A, and IL17A gene -737C > T polymorphisms in Turkish population. Methods In our study, peripheral blood samples collected from a total of 127 subjects, with 65 in the patient group and 62 in the control group, were analyzed for IL-17F gene 74488 T > C, IL-17A gene -197G > A, and IL17A gene -737C > T polymorphisms using next-generation sequencing. Results There was no statistically significant relationship between IL-17A gene -197G > A and IL-17A gene -737C > T polymorphisms and the risk of developing asthma. It was found that the risk of developing asthma was 2.9-fold higher in individuals with a C allele in the IL-17F gene 7488 T > C polymorphic site than the individuals with a T allele. It was shown that ATT and GCT haplotype carriers had a greater disease risk compared with the GTT haplotype carriers. Conclusions In conclusion, IL-17F gene 7488 T > C polymorphism was found to be associated with asthma in the Turkish population. The IL-17 gene should be further investigated as a potential candidate gene in predicting asthma susceptibility and in the treatment of asthma.