Browsing by Author "Sunguroglu, Asuman"

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    A Preliminary Investigation on the Presence of Calcifying Nanoparticles in the Breast Tumor
    (2014) Ozkal-Baydin, Pinar; Gocmen, Sedef J.; Erdemli, Esra; Tunc, Ibrahim G.; Sener, Hasan B.; Gumuskaya, Berrak; Sunguroglu, Asuman
    Calcium phosphate is deposited in many diseases, but the molecular basis of mineralization remains largely unknown. Biomineralizied calcifications that are formed by calcium deposits are also detected in breast mammograms. Some of the detected microcalcifications are thought to be related with malignancy. Taken together, calcifying nanoparticles (CNP) may be thought as a source of malign calcifications in breast cancers. The aim of the study is to research the presence of CNP in breast tumor tissue. With this aim, the presence of CNP was investigated by culturing 16 patients' breast tumor tissue and from 2 pathologic tissues with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Their growth was monitored by optical density (OD) at a wavelength of 650 nm. CNP couldn't be found in the analysed tissues. The presence of CNP in the breast tumor tissue was researched for the first time. We could not find CNP in the breast tumor tissue, but we think this research will open a new field of study for researchers.
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    The Role of Parkin in Rat Pancreatic Beta Cells Fate
    (2023) Verdi, Hasibe; Cebi, Hatice Pinar Baysan; Yalcin, Yaprak Yilmaz; Ozkan, Tulin; Mehtap, Akcil; Sunguroglu, Asuman; Atac, Fatma Belgin; 0000-0002-9337-9106; 0000-0001-7693-0958; 37715413; ABB-4078-2020; AAQ-4882-2020
    Parkin is a member of the mitochondrial quality control system that plays a major role in mitophagy. Although the loss of function mutations in the Parkin gene has been associated with the Familial Parkinson's pheno-type, research in recent years points out that Parkin's function is not limited to neurodegenerative diseases. Parkin's function impressing key cellular quality control mechanisms, including the ubiquitin-proteasome and autophagy-lysosome systems, makes it an important player in the maintenance of cellular homeostasis. In this study, we investigated whether Parkin affects cell viability and ER stress responses under lipotoxic conditions in INS-1E cells. Our results may suggest that silencing Parkin may affect autophagy in addition to apoptosis. We also showed that Parkin may have a protective effect against lipo-toxic effects in INS-1E cells. Consistent with previous studies, we observed that stress responses were different for high and low palmitic acid doses. The Parkin being inhibited under high-dose PA treatment and active under low-dose PA treatment indicate that regulation of stress responses is controlled by environmental conditions. Our preliminary findings may suggest that in low lipotoxic conditions, Parkin affects the ER stress response by modulating Chop activity and Ca2+ release from the ER to the cytoplasm.